##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727126.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5520107
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01061682318839	33.0	31.0	34.0	30.0	34.0
2	32.23473204414335	33.0	31.0	34.0	30.0	34.0
3	32.34283393419729	34.0	31.0	34.0	30.0	34.0
4	35.95552821711608	37.0	35.0	37.0	35.0	37.0
5	35.66470849206365	37.0	35.0	37.0	33.0	37.0
6	35.67700789133254	37.0	35.0	37.0	33.0	37.0
7	35.64233773004762	37.0	35.0	37.0	33.0	37.0
8	35.611728359613316	37.0	35.0	37.0	33.0	37.0
9	37.435121094572985	39.0	37.0	39.0	35.0	39.0
10	37.25907867365614	39.0	37.0	39.0	34.0	39.0
11	37.348291980572114	39.0	37.0	39.0	34.0	39.0
12	37.25412170452493	39.0	37.0	39.0	34.0	39.0
13	37.32624802381548	39.0	37.0	39.0	34.0	39.0
14	38.51933812152554	40.0	38.0	41.0	34.0	41.0
15	38.47035555651367	40.0	38.0	41.0	34.0	41.0
16	38.42910164603693	40.0	38.0	41.0	34.0	41.0
17	38.38765915225919	40.0	38.0	41.0	34.0	41.0
18	38.38715354611786	40.0	38.0	41.0	34.0	41.0
19	38.435648982891095	40.0	38.0	41.0	34.0	41.0
20	38.35031549207289	40.0	38.0	41.0	34.0	41.0
21	38.29312294127632	40.0	38.0	41.0	34.0	41.0
22	38.232698025599866	40.0	38.0	41.0	34.0	41.0
23	38.173774892406975	40.0	38.0	41.0	34.0	41.0
24	38.09822816840326	40.0	37.0	41.0	33.0	41.0
25	38.065204533172995	40.0	37.0	41.0	33.0	41.0
26	37.9072512543688	40.0	37.0	41.0	33.0	41.0
27	37.77782151686552	40.0	37.0	41.0	33.0	41.0
28	37.70140651259115	40.0	37.0	41.0	33.0	41.0
29	37.61068653198208	40.0	37.0	41.0	32.0	41.0
30	37.55853826746474	39.0	37.0	41.0	32.0	41.0
31	37.50998830276297	39.0	37.0	41.0	32.0	41.0
32	37.410348929830526	39.0	36.0	41.0	32.0	41.0
33	37.33637663182978	39.0	36.0	41.0	32.0	41.0
34	37.295445541182445	39.0	36.0	41.0	31.0	41.0
35	37.224411592021674	39.0	36.0	41.0	31.0	41.0
36	37.11145870904314	39.0	36.0	41.0	31.0	41.0
37	37.0319482937559	39.0	36.0	41.0	31.0	41.0
38	36.995737582622944	39.0	36.0	40.0	31.0	41.0
39	36.89677989937514	39.0	36.0	40.0	31.0	41.0
40	36.81462116585784	39.0	36.0	40.0	30.0	41.0
41	36.759263362105116	39.0	35.0	40.0	30.0	41.0
42	36.63106113703955	39.0	35.0	40.0	30.0	41.0
43	35.338655754317806	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	9.0
11	6.0
12	6.0
13	5.0
14	6.0
15	3.0
16	18.0
17	63.0
18	110.0
19	274.0
20	614.0
21	1252.0
22	2491.0
23	4601.0
24	7866.0
25	12894.0
26	20137.0
27	30363.0
28	44807.0
29	62528.0
30	86702.0
31	116277.0
32	153201.0
33	202478.0
34	272639.0
35	374008.0
36	534945.0
37	818895.0
38	1427134.0
39	1345771.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.6767089840831	18.41868282625681	12.796636007236817	26.10797218242328
2	18.418392976802807	20.637625321393227	35.95816168056163	24.98582002124234
3	20.017220680686084	22.339748124447585	29.98590425873991	27.65712693612642
4	15.173836304260044	15.59721577860719	33.84160850505253	35.38733941208024
5	15.614479936711373	35.317087150665735	33.72010723705174	15.348325675571145
6	35.28156247695923	33.877749108848796	15.223128827031795	15.617559587160176
7	29.1295440468817	30.129597125562963	21.319586739894717	19.421272087660622
8	26.173949164391196	34.90758421892909	19.692172633610184	19.22629398306953
9	26.54984767505412	15.059798659699894	19.74173689024506	38.648616775000924
10	17.506254860639476	26.40966923286088	32.849218321311525	23.234857585188113
11	35.454801872499935	21.86917753587023	21.37753851510487	21.298482076524966
12	21.390835358807355	24.792581013375283	30.247656431297436	23.568927196519923
13	30.785961214157627	19.806554474396965	24.32787987624153	25.079604435203883
14	22.22868143679099	20.75738749266998	25.711204511071976	31.302726559467054
15	25.216938729629696	27.440863012256827	22.679777765177377	24.662420492936096
16	24.324800225792725	26.135562227326393	24.84096775660327	24.698669790277616
17	23.936528766561953	26.246157909620234	25.59812699282822	24.219186330989597
18	24.019643097497926	25.410648742859514	26.347949414748662	24.221758744893897
19	24.99453362045337	25.246086715348092	26.575952241505462	23.183427422693075
20	24.46941698775042	25.179856115107913	26.417332127801146	23.93339476934052
21	24.070910219675092	25.554033644637684	26.668939569468492	23.706116566218736
22	24.65381558727032	25.078463152978735	26.002611905892405	24.265109353858538
23	24.063283555916577	25.33162853546136	26.23570521368517	24.369382694936892
24	24.005331780706424	25.30646597973554	26.294671461984343	24.393530777573698
25	24.41458109417082	25.055420121385332	26.078552462841753	24.4514463216021
26	23.98850239678325	25.444216932751484	26.3979846767463	24.16929599371896
27	24.37961800378145	25.69696565664397	26.007303843929115	23.916112495645464
28	24.098808229623085	25.68281738017035	25.91361000792195	24.30476438228462
29	23.966147757643103	25.856147353665428	26.39412605588986	23.78357883280161
30	24.296032667482713	25.63459367726024	26.078534347250876	23.99083930800617
31	24.118046987132676	25.114567525593255	26.457729895453113	24.309655591820956
32	23.63959611652455	25.68390431562287	26.286954220271454	24.389545347581123
33	23.76584366933467	25.10797345051464	26.399343346061947	24.72683953408874
34	24.08275781610755	25.067974225861928	26.790241565969648	24.05902639206088
35	23.862544693427136	25.001562469712997	26.68033427612907	24.455558560730797
36	23.605466343315445	24.659558229577797	27.086431476781158	24.6485439503256
37	23.359619659546453	24.274656270249835	27.511350776352707	24.854373293851005
38	23.29590712643795	24.108826151377137	27.910672746017422	24.68459397616749
39	22.897490936316995	24.095565538856402	28.505153976181987	24.501789548644616
40	23.12783067429671	23.9477966640864	28.520461650471628	24.403911011145254
41	22.46403919344317	24.132793078105188	28.72460624404563	24.678561484406007
42	22.11712562818076	24.48637318080972	28.591420419930262	24.805080771079258
43	21.933016878114863	24.168353982993445	28.50388588482071	25.394743254070978
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3656.0
1	3187.0
2	2718.0
3	4770.0
4	6822.0
5	6822.0
6	8082.0
7	9342.0
8	8576.0
9	7810.0
10	10960.5
11	14111.0
12	14111.0
13	22232.0
14	30353.0
15	36933.5
16	43514.0
17	39994.0
18	36474.0
19	36474.0
20	41901.5
21	47329.0
22	42057.0
23	36785.0
24	43329.0
25	49873.0
26	49873.0
27	59647.0
28	69421.0
29	80085.0
30	90749.0
31	104357.5
32	117966.0
33	117966.0
34	136050.0
35	154134.0
36	177019.0
37	199904.0
38	225436.5
39	250969.0
40	250969.0
41	278407.0
42	305845.0
43	334737.5
44	363630.0
45	393556.5
46	423483.0
47	423483.0
48	449455.0
49	475427.0
50	487099.0
51	498771.0
52	492511.5
53	486252.0
54	486252.0
55	460975.0
56	435698.0
57	406657.5
58	377617.0
59	344067.5
60	310518.0
61	310518.0
62	271715.0
63	232912.0
64	196634.5
65	160357.0
66	132952.5
67	105548.0
68	105548.0
69	85826.5
70	66105.0
71	54018.0
72	41931.0
73	34255.5
74	26580.0
75	26580.0
76	21357.5
77	16135.0
78	12944.5
79	9754.0
80	7714.5
81	5675.0
82	5675.0
83	4457.0
84	3239.0
85	2446.5
86	1654.0
87	1189.0
88	724.0
89	724.0
90	481.0
91	238.0
92	144.0
93	50.0
94	36.5
95	23.0
96	23.0
97	15.0
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5520107.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.57045600258738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.40206218952412	44.67979259420244
2	10.052684293234089	11.172645004501321
3	3.398222767638241	5.665223663880948
4	1.654085690753353	3.6767318441007424
5	0.9774951681337124	2.715992611675811
6	0.6388897521262855	2.130203691662262
7	0.4808011036946706	1.8702835605202097
8	0.34745686699128264	1.5446669231948742
9	0.2685172054385257	1.3429461195683379
>10	1.645859843281689	17.008233510174243
>50	0.10234419709384743	3.850190953666303
>100	0.030732447905034665	2.745230534333975
>500	4.2423817841278353E-4	0.1580075076666555
>1k	2.9370185088691605E-4	0.3754226221984622
>5k	3.263353898516017E-5	0.17363885650822616
>10k+	9.790061695548053E-5	0.8907900021452302
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18613	0.33718549296236466	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	17336	0.31405188341457874	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13172	0.23861856300973877	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9575	0.17345678263120623	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.81155908753218E-5
2	0.0	0.0	0.0	0.0	1.81155908753218E-5
3	0.0	0.0	0.0	0.0	1.81155908753218E-5
4	0.0	0.0	0.0	1.81155908753218E-5	1.81155908753218E-5
5	0.0	0.0	0.0	1.81155908753218E-5	1.81155908753218E-5
6	0.0	0.0	0.0	3.62311817506436E-5	5.43467726259654E-5
7	0.0	0.0	1.81155908753218E-5	3.62311817506436E-5	5.43467726259654E-5
8	0.0	0.0	1.81155908753218E-5	3.62311817506436E-5	1.2680913612725262E-4
9	0.0	0.0	1.81155908753218E-5	3.62311817506436E-5	2.3550268137918342E-4
10	0.0	0.0	1.81155908753218E-5	3.62311817506436E-5	2.3550268137918342E-4
11	0.0	0.0	1.81155908753218E-5	5.43467726259654E-5	2.3550268137918342E-4
12	1.81155908753218E-5	0.0	1.81155908753218E-5	5.43467726259654E-5	2.3550268137918342E-4
13	1.81155908753218E-5	0.0	1.81155908753218E-5	5.43467726259654E-5	2.3550268137918342E-4
14	1.81155908753218E-5	0.0	1.81155908753218E-5	1.2680913612725262E-4	3.62311817506436E-4
15	1.81155908753218E-5	0.0	1.81155908753218E-5	1.6304031787789622E-4	3.9854299925707964E-4
16	3.62311817506436E-5	0.0	1.81155908753218E-5	2.898494540051488E-4	4.166585901324014E-4
17	3.62311817506436E-5	0.0	1.81155908753218E-5	4.347741810077232E-4	4.166585901324014E-4
18	3.62311817506436E-5	0.0	1.81155908753218E-5	5.43467726259654E-4	4.166585901324014E-4
19	3.62311817506436E-5	0.0	1.81155908753218E-5	6.883924532622284E-4	4.52889771883045E-4
20	3.62311817506436E-5	0.0	1.81155908753218E-5	0.0010325886798933427	4.7100536275836684E-4
21	3.62311817506436E-5	0.0	1.81155908753218E-5	0.0015760564061529966	5.072365445090105E-4
22	3.62311817506436E-5	0.0	1.81155908753218E-5	0.002626760676921661	5.072365445090105E-4
23	5.43467726259654E-5	0.0	1.81155908753218E-5	0.0035687714024383948	6.702768623869066E-4
24	5.43467726259654E-5	0.0	1.81155908753218E-5	0.005416561671721219	6.702768623869066E-4
25	5.43467726259654E-5	0.0	1.81155908753218E-5	0.007608548167635156	6.702768623869066E-4
26	5.43467726259654E-5	0.0	1.81155908753218E-5	0.012644682430974616	6.702768623869066E-4
27	5.43467726259654E-5	0.0	1.81155908753218E-5	0.04233613587562705	6.702768623869066E-4
28	5.43467726259654E-5	0.0	1.81155908753218E-5	0.1233128270883155	6.702768623869066E-4
29	5.43467726259654E-5	0.0	1.81155908753218E-5	0.23738670283021687	6.702768623869066E-4
30	5.43467726259654E-5	0.0	1.81155908753218E-5	0.3773477579329531	6.702768623869066E-4
31	5.43467726259654E-5	0.0	1.81155908753218E-5	0.7623040640335413	6.883924532622284E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9430	0.0	16.852068	1
CTTATAC	5070	0.0	15.909271	37
TCTTATA	7655	0.0	13.074462	37
TATACGG	170	3.7388781E-7	13.058824	2
TCGTTAC	650	0.0	12.807693	23
GTATCAA	12600	0.0	12.641666	2
GTATACG	285	9.094947E-12	12.333333	1
CTCTTAT	11555	0.0	12.119862	37
TATACAC	2070	0.0	12.065218	37
ACCGTGC	465	0.0	11.935484	8
TATACTG	770	0.0	11.772727	5
ATACCCG	290	1.5643309E-10	11.4827585	5
TACTTAC	1250	0.0	11.1	31
AACGCAG	13885	0.0	11.098667	7
CGTTACG	620	0.0	11.040323	24
CTCGTTA	875	0.0	10.994285	22
GTACTAG	405	0.0	10.962963	1
TATTCCG	305	3.765308E-10	10.918033	5
TAGACAG	1000	0.0	10.915	5
GTAGTAC	475	0.0	10.905263	3
>>END_MODULE