FastQCFastQC Report
Thu 9 Feb 2017
SRR2727125.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727125.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8662741
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT267130.3083666012870522No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT261960.3023985133573773No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT198370.22899218619141445No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134660.155447334740817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC62950.017.39793437
GGTATCA135750.015.1815831
TCTTATA97200.013.77983537
CTCTTAT144600.012.06466137
TCGTTAC13500.012.05925923
GTTACGG20600.011.76456425
GGGTAAG42950.011.2852161
ACGGATG26500.011.23962228
GTATCAA182500.011.2115082
TTACGGA28350.010.83245126
CGTTACG12650.010.82213424
CTCGTTA16100.010.80124322
GTGCTAG12200.010.7663931
TACGGAT27850.010.76122127
GGCGTCA15950.010.67084712
GTATTAG9200.010.6576091
TACTTAC22350.010.59507831
TCTCTTA205950.010.59067737
ACCCGAC42800.010.37383311
CGGATGG31750.010.1968529
Produced by FastQC (version 0.11.5)