##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727125.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8662741
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77821269272624	31.0	31.0	34.0	30.0	34.0
2	32.010245948713	33.0	31.0	34.0	30.0	34.0
3	32.11791371807145	34.0	31.0	34.0	30.0	34.0
4	35.77564018132367	37.0	35.0	37.0	35.0	37.0
5	35.44555770511897	37.0	35.0	37.0	33.0	37.0
6	35.45897193509537	37.0	35.0	37.0	33.0	37.0
7	35.41927791677022	37.0	35.0	37.0	33.0	37.0
8	35.38401471312602	37.0	35.0	37.0	33.0	37.0
9	37.18167990939588	39.0	37.0	39.0	34.0	39.0
10	36.98152328460472	39.0	37.0	39.0	33.0	39.0
11	37.07751911317676	39.0	37.0	39.0	33.0	39.0
12	36.97357279872502	39.0	37.0	39.0	33.0	39.0
13	37.054472019883775	39.0	37.0	39.0	33.0	39.0
14	38.19105754171803	40.0	38.0	41.0	33.0	41.0
15	38.137232545680405	40.0	37.0	41.0	33.0	41.0
16	38.08461478878336	40.0	37.0	41.0	33.0	41.0
17	38.044930467158146	40.0	37.0	41.0	33.0	41.0
18	38.03972761046417	40.0	37.0	41.0	33.0	41.0
19	38.08970647973892	40.0	37.0	41.0	33.0	41.0
20	38.001567748591356	40.0	37.0	41.0	33.0	41.0
21	37.950860472453236	40.0	37.0	41.0	33.0	41.0
22	37.8882211761843	40.0	37.0	41.0	33.0	41.0
23	37.82812553209198	40.0	37.0	41.0	33.0	41.0
24	37.736681380639226	39.0	37.0	41.0	32.0	41.0
25	37.704668303023254	39.0	37.0	41.0	32.0	41.0
26	37.53678114121154	39.0	37.0	41.0	32.0	41.0
27	37.38931165089664	39.0	36.0	41.0	32.0	41.0
28	37.2959072653794	39.0	36.0	40.0	31.0	41.0
29	37.19364771496689	39.0	36.0	40.0	31.0	41.0
30	37.15388905197558	39.0	36.0	40.0	31.0	41.0
31	37.10357264519394	39.0	36.0	40.0	31.0	41.0
32	36.994782598256144	39.0	36.0	40.0	31.0	41.0
33	36.92485484675116	39.0	36.0	40.0	30.0	41.0
34	36.8850259981223	39.0	36.0	40.0	30.0	41.0
35	36.81181810699408	39.0	36.0	40.0	30.0	41.0
36	36.69838357166629	39.0	35.0	40.0	30.0	41.0
37	36.62280529915416	39.0	35.0	40.0	30.0	41.0
38	36.5838013626403	39.0	35.0	40.0	30.0	41.0
39	36.480799783809765	39.0	35.0	40.0	30.0	41.0
40	36.39886971109952	39.0	35.0	40.0	30.0	41.0
41	36.34579309250963	39.0	35.0	40.0	30.0	41.0
42	36.22698543105467	39.0	35.0	40.0	29.0	41.0
43	34.92306430493535	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	9.0
10	12.0
11	17.0
12	12.0
13	15.0
14	17.0
15	37.0
16	56.0
17	97.0
18	266.0
19	623.0
20	1352.0
21	2863.0
22	5293.0
23	9892.0
24	16931.0
25	27359.0
26	42009.0
27	62543.0
28	89027.0
29	124284.0
30	166750.0
31	219577.0
32	283333.0
33	367130.0
34	479877.0
35	644209.0
36	895489.0
37	1336508.0
38	2222265.0
39	1664887.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.23963292911562	18.75386785776003	12.890885229051635	26.11561398407271
2	18.468934947957003	20.821054213672095	35.84656403787208	24.863446800498824
3	19.93104722858504	22.66998401545192	30.014564674160294	27.384404081802742
4	15.138257048202181	15.710627848622046	34.10410169252434	35.04701341065143
5	15.514996927646804	35.57713430425774	33.77305173962837	15.134817028467088
6	35.187026831345875	34.258764056318896	15.18403932427392	15.370169788061308
7	29.10675731849769	30.27098466870936	21.41289921977351	19.209358793019437
8	26.172339678630586	35.10679818316166	19.68927617713608	19.031585961071677
9	26.704619242339117	14.980824198714934	19.654899067165925	38.659657491780024
10	17.24192146573469	26.521178458411722	33.01476980553846	23.222130270315134
11	35.544973582841735	21.953571046392824	21.375740080420275	21.125715290345166
12	21.412148879898407	24.857074683405635	30.183079466418306	23.547696970277652
13	30.830634322323615	19.91594808155987	24.317014672376793	24.936402923739724
14	22.22168479930313	20.775341199742666	25.70543203357921	31.297541967374993
15	25.342152097124917	27.498848228291717	22.597662795182263	24.561336879401104
16	24.300668806789908	26.220176731591078	24.862557936339087	24.61659652527993
17	23.944811463253952	26.27101514405198	25.53263453218791	24.251538860506162
18	24.016059120317692	25.483700828640725	26.35566502565412	24.14457502538746
19	25.02171079569388	25.27876569321419	26.552230985550647	23.14729252554128
20	24.33789720828546	25.126042669404526	26.49068002841133	24.045380093898686
21	24.026113674644087	25.58265334263139	26.777840870458903	23.613392112265622
22	24.658950325306968	25.025566388282876	25.92693236470997	24.388550921700187
23	24.068121163959535	25.312346288547698	26.234571713502692	24.384960833990075
24	23.949532832621916	25.300918035065344	26.310829332194047	24.43871980011869
25	24.424336361897463	25.010628852923112	26.08391501027215	24.481119774907274
26	24.00660483789138	25.35785151605017	26.396979893546398	24.23856375251205
27	24.463469472306745	25.738793298795382	25.913576314933113	23.88416091396476
28	24.12580498481947	25.665006029846673	25.885340448248424	24.323848537085432
29	24.00098306067329	25.898038507673267	26.330141926210192	23.770836505443256
30	24.393353096900857	25.578867012184713	26.03371150078249	23.994068390131947
31	24.263001745059675	25.045167574558675	26.433273256120664	24.25855742426098
32	23.67451595286065	25.694984993779684	26.24077067524009	24.38972837811958
33	23.836162249338862	25.101627764237673	26.322442284722587	24.73976770170088
34	24.21390642984709	25.05734616791614	26.801574697892967	23.92717270434381
35	24.01582824650997	25.119312697909358	26.597967086860848	24.266891968719833
36	23.76825071879674	24.801734231694102	26.901485338185687	24.528529711323472
37	23.50286127681758	24.499116388219385	27.346529233645562	24.65149310131747
38	23.57773365266259	24.245085937580267	27.588404178307997	24.588776231449145
39	23.052888225562786	24.342076024205273	28.145456501585354	24.45957924864659
40	23.439140105885656	24.072103737142783	28.251485297782768	24.237270859188794
41	22.85339016830816	24.240999471183542	28.376491920975127	24.529118439533168
42	22.50621367994264	24.505511592693352	28.32052810998274	24.667746617381265
43	22.263946249807077	24.163645201905492	28.250273210292214	25.322135337995217
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3693.0
1	3455.0
2	3217.0
3	5895.5
4	8574.0
5	8574.0
6	10337.0
7	12100.0
8	11317.0
9	10534.0
10	14503.5
11	18473.0
12	18473.0
13	29709.5
14	40946.0
15	51292.0
16	61638.0
17	57466.0
18	53294.0
19	53294.0
20	61828.0
21	70362.0
22	64225.0
23	58088.0
24	68520.5
25	78953.0
26	78953.0
27	96139.0
28	113325.0
29	130752.5
30	148180.0
31	172460.5
32	196741.0
33	196741.0
34	226949.5
35	257158.0
36	292098.5
37	327039.0
38	366873.0
39	406707.0
40	406707.0
41	449016.0
42	491325.0
43	534237.5
44	577150.0
45	618732.0
46	660314.0
47	660314.0
48	701433.0
49	742552.0
50	763065.5
51	783579.0
52	773453.0
53	763327.0
54	763327.0
55	720499.0
56	677671.0
57	628856.5
58	580042.0
59	528938.0
60	477834.0
61	477834.0
62	418393.5
63	358953.0
64	303397.0
65	247841.0
66	205870.5
67	163900.0
68	163900.0
69	133386.5
70	102873.0
71	83688.5
72	64504.0
73	52629.5
74	40755.0
75	40755.0
76	33166.0
77	25577.0
78	20828.5
79	16080.0
80	12628.5
81	9177.0
82	9177.0
83	7208.5
84	5240.0
85	4077.5
86	2915.0
87	2185.0
88	1455.0
89	1455.0
90	959.0
91	463.0
92	296.0
93	129.0
94	85.0
95	41.0
96	41.0
97	27.5
98	14.0
99	11.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8662741.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.46581171279282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.71639665561693	39.72486852331878
2	10.629257577555332	10.728282231113676
3	3.726398678168183	5.641672021777067
4	1.9012123511822614	3.837848981632006
5	1.0892738073259434	2.7485543432094013
6	0.7218393354159938	2.1856924792794628
7	0.5082900106549404	1.7955887581243974
8	0.37222094880800566	1.5027545854481528
9	0.29364020933029167	1.3336912363830813
>10	1.8183380520941954	17.22831998372314
>50	0.1539229423067549	5.346142385840318
>100	0.06659875932684255	5.7497462572074225
>500	0.0020154572746316126	0.6743489995182763
>1k	5.036434295980499E-4	0.5064753856012038
>5k	0.0	0.0
>10k+	9.157151004625945E-5	0.9960138278235418
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26713	0.3083666012870522	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	26196	0.3023985133573773	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19837	0.22899218619141445	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13466	0.155447334740817	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.617476154487361E-5
2	2.3087380772436806E-5	0.0	0.0	1.1543690386218403E-5	5.7718451931092015E-5
3	2.3087380772436806E-5	0.0	0.0	1.1543690386218403E-5	5.7718451931092015E-5
4	2.3087380772436806E-5	0.0	0.0	1.1543690386218403E-5	6.926214231731042E-5
5	3.463107115865521E-5	1.1543690386218403E-5	0.0	1.1543690386218403E-5	6.926214231731042E-5
6	3.463107115865521E-5	1.1543690386218403E-5	0.0	1.1543690386218403E-5	1.0389321347596563E-4
7	3.463107115865521E-5	1.1543690386218403E-5	0.0	1.1543690386218403E-5	1.0389321347596563E-4
8	4.617476154487361E-5	1.1543690386218403E-5	0.0	1.1543690386218403E-5	1.0389321347596563E-4
9	5.7718451931092015E-5	1.1543690386218403E-5	0.0	1.1543690386218403E-5	1.7315535579327606E-4
10	5.7718451931092015E-5	1.1543690386218403E-5	0.0	2.3087380772436806E-5	1.7315535579327606E-4
11	1.0389321347596563E-4	1.1543690386218403E-5	0.0	4.617476154487361E-5	1.7315535579327606E-4
12	1.1543690386218403E-4	1.1543690386218403E-5	0.0	5.7718451931092015E-5	1.7315535579327606E-4
13	1.1543690386218403E-4	1.1543690386218403E-5	0.0	5.7718451931092015E-5	1.7315535579327606E-4
14	1.1543690386218403E-4	1.1543690386218403E-5	0.0	8.080583270352882E-5	2.539611884968049E-4
15	1.1543690386218403E-4	1.1543690386218403E-5	0.0	1.2698059424840245E-4	3.001359500416785E-4
16	1.1543690386218403E-4	1.1543690386218403E-5	0.0	2.4241749811058648E-4	3.693980923589889E-4
17	1.1543690386218403E-4	1.1543690386218403E-5	0.0	3.116796404278969E-4	3.693980923589889E-4
18	1.1543690386218403E-4	1.1543690386218403E-5	0.0	3.693980923589889E-4	4.2711654429008095E-4
19	1.1543690386218403E-4	1.1543690386218403E-5	0.0	5.194660673798282E-4	4.2711654429008095E-4
20	1.1543690386218403E-4	4.617476154487361E-5	0.0	7.965146366490698E-4	4.2711654429008095E-4
21	1.1543690386218403E-4	4.617476154487361E-5	0.0	0.0012351748713253692	4.5020392506251773E-4
22	1.1543690386218403E-4	4.617476154487361E-5	0.0	0.0017661846290914157	4.5020392506251773E-4
23	1.1543690386218403E-4	4.617476154487361E-5	0.0	0.0022163885541539334	6.233592808557938E-4
24	1.1543690386218403E-4	4.617476154487361E-5	0.0	0.0032553206889135897	6.233592808557938E-4
25	1.1543690386218403E-4	5.7718451931092015E-5	0.0	0.00412109746787997	6.464466616282306E-4
26	1.1543690386218403E-4	5.7718451931092015E-5	0.0	0.006729971495165329	6.695340424006675E-4
27	1.1543690386218403E-4	5.7718451931092015E-5	0.0	0.022960400178188404	6.695340424006675E-4
28	1.1543690386218403E-4	5.7718451931092015E-5	0.0	0.07211343384270637	6.695340424006675E-4
29	1.1543690386218403E-4	5.7718451931092015E-5	0.0	0.1468357417126981	6.695340424006675E-4
30	1.1543690386218403E-4	5.7718451931092015E-5	0.0	0.24570744987065873	6.695340424006675E-4
31	1.1543690386218403E-4	5.7718451931092015E-5	0.0	0.5570638669677415	6.810777327868858E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	6295	0.0	17.397934	37
GGTATCA	13575	0.0	15.181583	1
TCTTATA	9720	0.0	13.779835	37
CTCTTAT	14460	0.0	12.064661	37
TCGTTAC	1350	0.0	12.059259	23
GTTACGG	2060	0.0	11.764564	25
GGGTAAG	4295	0.0	11.285216	1
ACGGATG	2650	0.0	11.239622	28
GTATCAA	18250	0.0	11.211508	2
TTACGGA	2835	0.0	10.832451	26
CGTTACG	1265	0.0	10.822134	24
CTCGTTA	1610	0.0	10.801243	22
GTGCTAG	1220	0.0	10.766393	1
TACGGAT	2785	0.0	10.761221	27
GGCGTCA	1595	0.0	10.670847	12
GTATTAG	920	0.0	10.657609	1
TACTTAC	2235	0.0	10.595078	31
TCTCTTA	20595	0.0	10.590677	37
ACCCGAC	4280	0.0	10.373833	11
CGGATGG	3175	0.0	10.19685	29
>>END_MODULE