FastQCFastQC Report
Thu 9 Feb 2017
SRR2727124.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727124.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9553423
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT250500.26220968128387073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT239670.2508734303924363No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT198720.20800921303285744No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144560.15131749112333873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105600.018.7627831
CTTATAC68250.016.48058737
TCTTATA108300.013.54616837
GTATCAA147450.013.4499832
GTACTAG7050.012.3333331
CTCTTAT159450.011.98526137
TCTATAC10300.011.4951463
TACTTAC21600.011.39120431
TCGTTAC11800.011.28813623
TATACTG15500.011.2193565
CTATACT13050.011.0574714
GGGTAAG42250.010.8153851
GTATTAT10250.010.4682921
GTTACGG17000.010.44705925
TACGGAT23300.010.40128827
GTACTTA25150.010.2982130
AACGCAG191000.010.2960737
GGAGTGT33100.010.2280976
GAGTGTC29000.010.2068977
GTAAGAG43850.010.1254283
Produced by FastQC (version 0.11.5)