##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727124.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9553423
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.959515034558816	33.0	31.0	34.0	30.0	34.0
2	32.190946323637085	33.0	31.0	34.0	30.0	34.0
3	32.308128824610826	34.0	31.0	34.0	30.0	34.0
4	35.92311645783925	37.0	35.0	37.0	35.0	37.0
5	35.63196919051946	37.0	35.0	37.0	33.0	37.0
6	35.641729775809154	37.0	35.0	37.0	33.0	37.0
7	35.605474079814115	37.0	35.0	37.0	33.0	37.0
8	35.57000626895721	37.0	35.0	37.0	33.0	37.0
9	37.38774384846143	39.0	37.0	39.0	34.0	39.0
10	37.21061016559196	39.0	37.0	39.0	34.0	39.0
11	37.29819489831027	39.0	37.0	39.0	34.0	39.0
12	37.202301101919176	39.0	37.0	39.0	34.0	39.0
13	37.27316324211751	39.0	37.0	39.0	34.0	39.0
14	38.46686376181605	40.0	38.0	41.0	34.0	41.0
15	38.41712651057113	40.0	38.0	41.0	34.0	41.0
16	38.37832481614181	40.0	38.0	41.0	34.0	41.0
17	38.33059103527605	40.0	38.0	41.0	34.0	41.0
18	38.328937596503366	40.0	38.0	41.0	34.0	41.0
19	38.378993791021294	40.0	38.0	41.0	34.0	41.0
20	38.29447497509531	40.0	38.0	41.0	34.0	41.0
21	38.2392061986578	40.0	38.0	41.0	34.0	41.0
22	38.18136473178252	40.0	38.0	41.0	34.0	41.0
23	38.12490423589534	40.0	38.0	41.0	33.0	41.0
24	38.045332861321015	40.0	37.0	41.0	33.0	41.0
25	38.01452170598957	40.0	37.0	41.0	33.0	41.0
26	37.8586395682469	40.0	37.0	41.0	33.0	41.0
27	37.73123832159426	40.0	37.0	41.0	33.0	41.0
28	37.65165752631282	40.0	37.0	41.0	32.0	41.0
29	37.56682698965596	39.0	37.0	41.0	32.0	41.0
30	37.517491479232106	39.0	37.0	41.0	32.0	41.0
31	37.477925451432434	39.0	37.0	41.0	32.0	41.0
32	37.37786958663926	39.0	36.0	41.0	32.0	41.0
33	37.30849738360795	39.0	36.0	41.0	31.0	41.0
34	37.27219489810092	39.0	36.0	41.0	31.0	41.0
35	37.20004044623587	39.0	36.0	41.0	31.0	41.0
36	37.08959437889435	39.0	36.0	41.0	31.0	41.0
37	37.01627060792765	39.0	36.0	40.0	31.0	41.0
38	36.982277242408294	39.0	36.0	40.0	31.0	41.0
39	36.88871234948981	39.0	36.0	40.0	30.0	41.0
40	36.81515829457148	39.0	36.0	40.0	30.0	41.0
41	36.76494058726385	39.0	35.0	40.0	30.0	41.0
42	36.64418460273349	39.0	35.0	40.0	30.0	41.0
43	35.34569043996063	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	1.0
10	11.0
11	10.0
12	10.0
13	5.0
14	11.0
15	42.0
16	56.0
17	106.0
18	208.0
19	478.0
20	1084.0
21	2358.0
22	4623.0
23	8417.0
24	14232.0
25	23088.0
26	36359.0
27	54806.0
28	79812.0
29	112850.0
30	155556.0
31	208230.0
32	272047.0
33	359417.0
34	479688.0
35	651376.0
36	920286.0
37	1401002.0
38	2455511.0
39	2311740.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.77833191307451	18.24179668376455	12.397566819767114	26.582304583393828
2	18.309248946686438	20.344278694662634	36.06772148579624	25.278750872854683
3	19.757871079297963	22.356635940855963	29.7243616241006	28.16113135574547
4	14.855115281716301	15.322821987469831	34.249357533943595	35.572705196870274
5	15.506117545512222	35.499108539420895	33.809483783979836	15.185290131087045
6	35.42308343302709	34.193880036506286	15.232948441621396	15.150088088845223
7	29.57479219751915	30.040101856685297	21.319761513752713	19.06534443204284
8	26.379298812582675	35.10397268078677	19.58744002018962	18.929288486440935
9	26.920738252666087	14.845705042056654	19.42565507671962	38.80790162855764
10	17.209370923908633	26.21845593982387	33.00154300715042	23.57063012911707
11	35.88344198723327	21.671300433362994	21.42117019208717	21.024087387316566
12	21.61023331637257	24.535572223694064	30.358312408023803	23.49588205190956
13	30.88591387610493	19.68087249983592	24.373871019842834	25.05934260421631
14	22.3769009285991	20.271351954163443	25.628803414231736	31.72294370300572
15	25.29078844305334	27.23070045155543	22.551435229027334	24.927075876363897
16	24.65857525621968	25.97126705265746	24.53658756657169	24.83357012455117
17	24.189832272683834	25.971664815846633	25.388763797018093	24.44973911445144
18	24.32708150785326	25.216668412986632	26.161471129248646	24.29477894991146
19	25.275631572055378	24.97152067902782	26.265549007931504	23.4872987409853
20	24.615250470956852	24.86322441704926	26.266878374379527	24.25464673761436
21	24.291063004328397	25.21680448986714	26.58998769341628	23.902144812388187
22	24.860377270010968	24.75370346314614	25.849132818676612	24.536786448166275
23	24.320832438802302	25.005979532153034	26.069975128286476	24.60321290075819
24	24.185100984223144	25.096324113357067	26.114639747449687	24.60393515497011
25	24.67249696784074	24.804889305121318	25.91410429539234	24.6085094316456
26	24.28644685784352	25.13882196988451	26.114713019616108	24.46001815265586
27	24.654911647898352	25.482761519091113	25.688342283179548	24.173984549830987
28	24.368417477170226	25.312477004315625	25.725093508368673	24.594012010145473
29	24.27003389256395	25.612557928189716	26.102790591393266	24.014617587853067
30	24.584852989342146	25.367033365946423	25.819918159177078	24.22819548553435
31	24.470035504551614	24.792453971733483	26.207601191740387	24.52990933197452
32	23.894440767461045	25.40966729935438	26.10567960824094	24.590212324943636
33	24.071016221096876	24.86322441704926	26.21097171139601	24.85478765045785
34	24.451497646445677	24.77205290710984	26.60104132309435	24.175408123350135
35	24.266045793219877	24.877355477717252	26.426255803809795	24.430342925253072
36	24.015570126016613	24.574291329924364	26.691888342011026	24.718250202047997
37	23.843171185867096	24.230990295310907	26.98462111433776	24.941217404484238
38	23.770663143461775	24.099299277337558	27.276506023024417	24.853531556176254
39	23.408960327622886	24.119030425011015	27.805981165075593	24.666028082290506
40	23.70491707527239	23.861928860472315	27.9382688278327	24.49488523642259
41	23.10652422697079	24.047150429746488	28.114739606945072	24.731585736337646
42	22.813843791905793	24.224437670141896	28.092360193827908	24.869358344124404
43	22.558574031527755	23.924283474101376	27.979510590078547	25.53763190429231
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3857.0
1	3722.5
2	3588.0
3	7047.0
4	10506.0
5	10506.0
6	12248.0
7	13990.0
8	12305.0
9	10620.0
10	14804.5
11	18989.0
12	18989.0
13	30231.5
14	41474.0
15	49344.0
16	57214.0
17	54427.5
18	51641.0
19	51641.0
20	59767.0
21	67893.0
22	62866.0
23	57839.0
24	69330.5
25	80822.0
26	80822.0
27	98230.5
28	115639.0
29	134512.0
30	153385.0
31	179090.0
32	204795.0
33	204795.0
34	237336.5
35	269878.0
36	309075.5
37	348273.0
38	393219.0
39	438165.0
40	438165.0
41	486341.5
42	534518.0
43	584075.0
44	633632.0
45	682267.0
46	730902.0
47	730902.0
48	775450.5
49	819999.0
50	841212.5
51	862426.0
52	852791.0
53	843156.0
54	843156.0
55	804220.0
56	765284.0
57	714191.0
58	663098.0
59	605179.0
60	547260.0
61	547260.0
62	479862.5
63	412465.0
64	349232.0
65	285999.0
66	238524.5
67	191050.0
68	191050.0
69	155281.5
70	119513.0
71	97964.5
72	76416.0
73	62067.5
74	47719.0
75	47719.0
76	38701.5
77	29684.0
78	24187.5
79	18691.0
80	14826.5
81	10962.0
82	10962.0
83	8582.5
84	6203.0
85	4830.5
86	3458.0
87	2527.5
88	1597.0
89	1597.0
90	1079.5
91	562.0
92	360.0
93	158.0
94	117.0
95	76.0
96	76.0
97	49.5
98	23.0
99	13.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9553423.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.330175286116734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.52621126739422	38.73711766375192
2	10.588625277009557	10.446774819077756
3	3.7825585410794873	5.597828275843473
4	1.8848298996943382	3.719159573457421
5	1.0936766123273625	2.6975629496217564
6	0.7253400266497434	2.1468690394001055
7	0.5367670394169366	1.853516849957319
8	0.372590485289507	1.4703963159416542
9	0.29295483088068314	1.3006361842432903
>10	1.955631155993769	18.247028584511966
>50	0.1659776470378961	5.651013093131754
>100	0.07209149039677354	6.0448282206595145
>500	0.0021926768337346613	0.7380986290652164
>1k	4.254232163552288E-4	0.3622426280353796
>5k	4.25422598465112E-5	0.11238094759509665
>10k+	8.50845196930224E-5	0.8745462257061933
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	25050	0.26220968128387073	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23967	0.2508734303924363	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19872	0.20800921303285744	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14456	0.15131749112333873	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.1869809386646025E-5
2	0.0	0.0	0.0	0.0	4.1869809386646025E-5
3	0.0	0.0	0.0	2.0934904693323013E-5	4.1869809386646025E-5
4	0.0	0.0	0.0	2.0934904693323013E-5	6.280471407996903E-5
5	0.0	0.0	0.0	3.1402357039984514E-5	6.280471407996903E-5
6	0.0	0.0	0.0	3.1402357039984514E-5	1.0467452346661506E-4
7	0.0	0.0	0.0	3.1402357039984514E-5	1.0467452346661506E-4
8	1.0467452346661506E-5	0.0	0.0	3.1402357039984514E-5	1.3607688050659957E-4
9	2.0934904693323013E-5	0.0	0.0	4.1869809386646025E-5	1.988815945865686E-4
10	2.0934904693323013E-5	0.0	0.0	4.1869809386646025E-5	1.988815945865686E-4
11	3.1402357039984514E-5	0.0	0.0	6.280471407996903E-5	1.988815945865686E-4
12	4.1869809386646025E-5	0.0	0.0	6.280471407996903E-5	2.0934904693323012E-4
13	4.1869809386646025E-5	0.0	0.0	7.327216642663053E-5	2.0934904693323012E-4
14	4.1869809386646025E-5	0.0	0.0	8.373961877329205E-5	2.512188563198761E-4
15	4.1869809386646025E-5	0.0	0.0	1.4654433285326107E-4	3.035561180531837E-4
16	5.233726173330753E-5	0.0	0.0	2.7215376101319915E-4	3.454259274398297E-4
17	5.233726173330753E-5	0.0	0.0	4.1869809386646024E-4	3.454259274398297E-4
18	6.280471407996903E-5	1.0467452346661506E-5	0.0	5.338400696797368E-4	4.1869809386646024E-4
19	8.373961877329205E-5	1.0467452346661506E-5	0.0	6.803844025329978E-4	4.1869809386646024E-4
20	9.420707111995356E-5	1.0467452346661506E-5	0.0	9.10668354159551E-4	4.1869809386646024E-4
21	1.0467452346661506E-4	1.0467452346661506E-5	0.0	0.0014863782332259338	4.2916554621312173E-4
22	1.2560942815993806E-4	1.0467452346661506E-5	0.0	0.0023028395162655313	4.2916554621312173E-4
23	1.2560942815993806E-4	1.0467452346661506E-5	0.0	0.002941354109411883	5.757098790663828E-4
24	1.2560942815993806E-4	1.0467452346661506E-5	0.0	0.004145111129277956	5.757098790663828E-4
25	1.2560942815993806E-4	1.0467452346661506E-5	0.0	0.005254661078024076	5.861773314130443E-4
26	1.2560942815993806E-4	1.0467452346661506E-5	0.0	0.008049470854582698	5.966447837597058E-4
27	1.2560942815993806E-4	1.0467452346661506E-5	0.0	0.0264303171753203	5.966447837597058E-4
28	1.2560942815993806E-4	1.0467452346661506E-5	0.0	0.08475496165091821	5.966447837597058E-4
29	1.2560942815993806E-4	1.0467452346661506E-5	0.0	0.16624407816967804	6.071122361063674E-4
30	1.2560942815993806E-4	1.0467452346661506E-5	0.0	0.26652227165069525	6.071122361063674E-4
31	1.2560942815993806E-4	1.0467452346661506E-5	0.0	0.5750504295685431	6.280471407996903E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10560	0.0	18.762783	1
CTTATAC	6825	0.0	16.480587	37
TCTTATA	10830	0.0	13.546168	37
GTATCAA	14745	0.0	13.449983	2
GTACTAG	705	0.0	12.333333	1
CTCTTAT	15945	0.0	11.985261	37
TCTATAC	1030	0.0	11.495146	3
TACTTAC	2160	0.0	11.391204	31
TCGTTAC	1180	0.0	11.288136	23
TATACTG	1550	0.0	11.219356	5
CTATACT	1305	0.0	11.057471	4
GGGTAAG	4225	0.0	10.815385	1
GTATTAT	1025	0.0	10.468292	1
GTTACGG	1700	0.0	10.447059	25
TACGGAT	2330	0.0	10.401288	27
GTACTTA	2515	0.0	10.29821	30
AACGCAG	19100	0.0	10.296073	7
GGAGTGT	3310	0.0	10.228097	6
GAGTGTC	2900	0.0	10.206897	7
GTAAGAG	4385	0.0	10.125428	3
>>END_MODULE