FastQCFastQC Report
Thu 9 Feb 2017
SRR2727123.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727123.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4285299
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT239520.5589341607201738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT228760.5338250609817425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT161420.3766831672655747No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123360.28786789439896726No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45850.10699370102296246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC36550.014.83036937
GGTATCA129250.014.198841
ATACCCG3100.013.1290325
CGAACCG1307.006496E-512.80769318
TATACAC17200.012.6918637
TCTTATA55800.012.66487537
CGCGAAC1205.1265035E-412.33333412
ATACCGT1951.4703437E-712.3333346
CTCTTAT82900.012.00603237
ACCGACC3300.011.7727278
TCGTTAC4900.011.704082523
TCTATAC5100.011.2450983
GGGTAAG13900.011.1798561
TAAGGCG2851.4224497E-911.0350885
GTATCAA168350.010.9120872
TCTCTTA118100.010.73031337
ACCCGTT4000.010.6374997
GTCATAC4050.010.5061731
TACCCGT3709.094947E-1210.5000016
CGACCGA3251.1350494E-910.24615418
Produced by FastQC (version 0.11.5)