##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727123.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4285299
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4189856997143	31.0	31.0	34.0	30.0	34.0
2	31.645750039845527	31.0	31.0	34.0	30.0	34.0
3	31.731727471058612	31.0	31.0	34.0	30.0	34.0
4	35.47039938170008	37.0	35.0	37.0	33.0	37.0
5	34.99586329915369	37.0	35.0	37.0	32.0	37.0
6	35.01529951585642	37.0	35.0	37.0	32.0	37.0
7	34.9733563515638	36.0	35.0	37.0	32.0	37.0
8	34.934897891605694	36.0	35.0	37.0	32.0	37.0
9	36.668383933069784	38.0	35.0	39.0	32.0	39.0
10	36.413609412085364	38.0	35.0	39.0	32.0	39.0
11	36.538894018830426	38.0	35.0	39.0	32.0	39.0
12	36.41334221952774	38.0	35.0	39.0	32.0	39.0
13	36.518899614706	38.0	35.0	39.0	32.0	39.0
14	37.520162070371285	39.0	37.0	40.0	32.0	41.0
15	37.45950609280706	39.0	36.0	40.0	32.0	41.0
16	37.386772311570326	39.0	36.0	40.0	32.0	41.0
17	37.351963305244276	39.0	36.0	40.0	32.0	41.0
18	37.351887231206035	39.0	36.0	40.0	32.0	41.0
19	37.40039983207706	39.0	36.0	40.0	32.0	41.0
20	37.296335681594215	39.0	36.0	40.0	32.0	41.0
21	37.22420839245989	39.0	36.0	40.0	31.0	41.0
22	37.149722341428216	39.0	36.0	40.0	31.0	41.0
23	37.073406313071736	39.0	36.0	40.0	31.0	41.0
24	36.95077076302027	39.0	36.0	40.0	31.0	41.0
25	36.90815460018076	39.0	36.0	40.0	31.0	41.0
26	36.68121104268337	39.0	36.0	40.0	30.0	41.0
27	36.49744626921015	38.0	35.0	40.0	30.0	41.0
28	36.3816482817185	38.0	35.0	40.0	30.0	41.0
29	36.23473601258629	38.0	35.0	40.0	30.0	41.0
30	36.16652793655705	38.0	35.0	40.0	30.0	41.0
31	36.08872286391218	38.0	35.0	40.0	29.0	41.0
32	35.94608777590548	38.0	35.0	40.0	29.0	41.0
33	35.83645178551135	38.0	35.0	40.0	29.0	41.0
34	35.773080944876895	38.0	34.0	40.0	28.0	41.0
35	35.66508521342385	38.0	34.0	40.0	27.0	41.0
36	35.504401676522455	38.0	34.0	40.0	27.0	41.0
37	35.39439371675115	38.0	34.0	40.0	27.0	41.0
38	35.32618774092543	38.0	34.0	40.0	26.0	41.0
39	35.18474206817307	38.0	34.0	40.0	26.0	41.0
40	35.056770134359354	38.0	34.0	40.0	25.0	41.0
41	34.971130135843495	38.0	33.0	40.0	25.0	41.0
42	34.79601936761006	38.0	33.0	40.0	24.0	41.0
43	33.48949629885803	37.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	8.0
11	14.0
12	19.0
13	15.0
14	26.0
15	34.0
16	71.0
17	188.0
18	381.0
19	839.0
20	1690.0
21	3243.0
22	6014.0
23	10231.0
24	16154.0
25	24776.0
26	36033.0
27	50944.0
28	69041.0
29	91071.0
30	117225.0
31	146999.0
32	184799.0
33	230925.0
34	294494.0
35	385373.0
36	524303.0
37	734854.0
38	954409.0
39	401119.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.21336714194272	19.35799578979203	13.482419779809998	24.94621728845525
2	18.771758983445498	21.842046494305297	35.26108679931085	24.125107722938353
3	20.5705599539262	23.21560292525679	30.202396612231723	26.01144050858528
4	15.575995980677193	16.369546209027654	33.60439959965454	34.45005821064061
5	15.507202647936586	35.77456322184286	33.468679781737514	15.249554348483036
6	34.64444371326248	34.34507137074916	15.082191464352896	15.928293451635463
7	28.376829714799364	30.5685787619487	21.662409087440572	19.392182435811364
8	26.13406905795838	34.934481817954826	19.78060340713682	19.15084571694997
9	26.25975457021785	15.105223696176159	20.100721093207266	38.53430064039872
10	17.54351329977208	26.987568428714077	32.73937244518994	22.729545826323903
11	34.83572558180888	22.295947143944915	21.43470035579781	21.433626918448397
12	21.219172804511423	25.671277546794286	30.277397213123287	22.832152435571007
13	30.777292319625772	20.11959958920019	24.676947862914584	24.42616022825945
14	21.843633314734863	21.435073725310648	26.51324913384107	30.208043826113418
15	24.921761585364287	27.856305942712517	23.308501927170074	23.91343054475312
16	23.576347881443045	26.48940015620847	25.666587092289245	24.267664870059242
17	23.409008332907458	26.720469213466785	26.26143473302563	23.609087720600126
18	23.474978058707222	25.51180209362287	27.178313578585765	23.83490626908414
19	24.520879406547827	25.457453493910226	27.623673400619186	22.397993698922757
20	23.95503790984013	25.478735556142055	27.412766297054187	23.153460236963628
21	23.520249112138966	25.892755674691543	27.683389187078895	22.903606026090596
22	23.89959253718352	25.688452544384884	27.00184981258017	23.41010510585142
23	23.384669307789256	25.809960985219465	27.174369863106403	23.630999843884872
24	23.36819437803523	25.86265275771889	27.21579054343699	23.553362320808887
25	23.698556390114202	25.55443622487019	27.09316199406389	23.653845390951716
26	23.313635757971614	25.96131565148663	27.38065651895002	23.344392071591738
27	23.666213256064513	26.144756760263405	27.10667330330976	23.082356680362327
28	23.397620562765866	26.21021310298301	27.057598547965966	23.334567786285156
29	23.296927472272063	26.39722455772631	27.319820623951795	22.98602734604983
30	23.40875164136738	26.269112143633382	27.17406650037722	23.148069714622014
31	23.36968785608659	25.712698227124875	27.420140344932758	23.497473571855778
32	22.879757048457996	26.25191381044823	27.247293596082795	23.62103554501098
33	23.084946931357646	25.73115668241586	27.31347334223353	23.870423043992965
34	23.284862969888447	25.752858785349638	27.568251363557128	23.394026881204788
35	23.158314040630536	25.743501211934106	27.50601533288576	23.592169414549602
36	22.9594947750437	25.45836358209777	27.802097356567185	23.78004428629134
37	22.729475819540244	25.07530979751938	28.15859523454489	24.03661914839548
38	22.615598118124314	24.95184116674239	28.524240665587165	23.908320049546134
39	22.266917664321674	24.894178912603298	29.066443205013233	23.7724602180618
40	22.457686149787914	24.810217443403598	29.070130228952518	23.661966177855966
41	21.984113593940585	24.85184814408516	29.195862412401098	23.968175849573157
42	21.57751419445878	25.246546390345227	28.999400041864053	24.17653937333194
43	21.52372564901539	24.918377924154182	28.92491282405265	24.632983602777774
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4596.0
1	3765.0
2	2934.0
3	4953.0
4	6972.0
5	6972.0
6	8587.5
7	10203.0
8	9957.5
9	9712.0
10	13665.5
11	17619.0
12	17619.0
13	28109.0
14	38599.0
15	48478.0
16	58357.0
17	52051.5
18	45746.0
19	45746.0
20	50201.0
21	54656.0
22	45333.5
23	36011.0
24	41246.5
25	46482.0
26	46482.0
27	53751.0
28	61020.0
29	68977.5
30	76935.0
31	87618.5
32	98302.0
33	98302.0
34	111709.5
35	125117.0
36	142009.0
37	158901.0
38	177708.5
39	196516.0
40	196516.0
41	216703.0
42	236890.0
43	258590.0
44	280290.0
45	300790.5
46	321291.0
47	321291.0
48	340028.5
49	358766.0
50	365016.5
51	371267.0
52	365352.0
53	359437.0
54	359437.0
55	338725.5
56	318014.0
57	295729.5
58	273445.0
59	248780.0
60	224115.0
61	224115.0
62	195961.5
63	167808.0
64	142199.0
65	116590.0
66	96780.5
67	76971.0
68	76971.0
69	63044.0
70	49117.0
71	40635.0
72	32153.0
73	26323.5
74	20494.0
75	20494.0
76	16511.5
77	12529.0
78	10169.5
79	7810.0
80	6110.0
81	4410.0
82	4410.0
83	3510.0
84	2610.0
85	2063.0
86	1516.0
87	1120.0
88	724.0
89	724.0
90	495.0
91	266.0
92	165.5
93	65.0
94	47.0
95	29.0
96	29.0
97	20.5
98	12.0
99	7.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4285299.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.50461167855904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.59672516287236	50.579873935788164
2	8.62720011250834	10.43970785361076
3	2.839090845775626	5.153342674314181
4	1.3519093610053514	3.271870036489507
5	0.825464254306377	2.4972197080669325
6	0.5240592381933875	1.902480042207143
7	0.38869084187273195	1.646231191536529
8	0.2703142275879552	1.3084205897119088
9	0.21380240037282994	1.1642428089451695
>10	1.2672210768767378	13.833887863455043
>50	0.0687656615440319	2.8125553859660313
>100	0.02532794100787542	2.65706658288405
>500	8.496021131049154E-4	0.33315011335691064
>1k	4.248009064550485E-4	0.6403639590483665
>5k	0.0	0.0
>10k+	1.5447305689274236E-4	1.7595872546192781
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23952	0.5589341607201738	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22876	0.5338250609817425	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16142	0.3766831672655747	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12336	0.28786789439896726	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4585	0.10699370102296246	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3335594552445467E-5
2	0.0	0.0	0.0	0.0	2.3335594552445467E-5
3	0.0	0.0	0.0	0.0	2.3335594552445467E-5
4	0.0	0.0	0.0	2.3335594552445467E-5	2.3335594552445467E-5
5	0.0	2.3335594552445467E-5	0.0	2.3335594552445467E-5	2.3335594552445467E-5
6	2.3335594552445467E-5	2.3335594552445467E-5	0.0	2.3335594552445467E-5	7.000678365733639E-5
7	7.000678365733639E-5	2.3335594552445467E-5	0.0	2.3335594552445467E-5	7.000678365733639E-5
8	9.334237820978187E-5	2.3335594552445467E-5	0.0	2.3335594552445467E-5	9.334237820978187E-5
9	1.4001356731467278E-4	2.3335594552445467E-5	0.0	2.3335594552445467E-5	1.4001356731467278E-4
10	1.6334916186711826E-4	2.3335594552445467E-5	0.0	7.000678365733639E-5	1.4001356731467278E-4
11	1.6334916186711826E-4	2.3335594552445467E-5	0.0	1.1667797276222733E-4	1.4001356731467278E-4
12	1.6334916186711826E-4	2.3335594552445467E-5	0.0	1.1667797276222733E-4	1.4001356731467278E-4
13	1.6334916186711826E-4	2.3335594552445467E-5	0.0	1.1667797276222733E-4	1.4001356731467278E-4
14	1.8668475641956373E-4	2.3335594552445467E-5	0.0	1.6334916186711826E-4	1.8668475641956373E-4
15	2.1002035097200918E-4	2.3335594552445467E-5	0.0	2.3335594552445466E-4	2.3335594552445466E-4
16	2.3335594552445466E-4	2.3335594552445467E-5	0.0	2.566915400769001E-4	2.566915400769001E-4
17	2.3335594552445466E-4	2.3335594552445467E-5	0.0	3.967051073915729E-4	2.566915400769001E-4
18	2.3335594552445466E-4	2.3335594552445467E-5	0.0	5.833898638111367E-4	2.8002713462934556E-4
19	2.3335594552445466E-4	2.3335594552445467E-5	0.0	7.467390256782549E-4	2.8002713462934556E-4
20	2.3335594552445466E-4	2.3335594552445467E-5	0.0	0.0011201085385173822	2.8002713462934556E-4
21	2.3335594552445466E-4	2.3335594552445467E-5	0.0	0.0023802306443494376	3.0336272918179107E-4
22	2.3335594552445466E-4	2.3335594552445467E-5	0.0	0.003943715479363284	3.0336272918179107E-4
23	2.3335594552445466E-4	2.3335594552445467E-5	0.0	0.00473712569414643	3.5003391828668197E-4
24	2.3335594552445466E-4	4.6671189104890934E-5	0.0	0.007397383473125212	3.5003391828668197E-4
25	2.3335594552445466E-4	4.6671189104890934E-5	0.0	0.010150983630313777	3.5003391828668197E-4
26	2.3335594552445466E-4	4.6671189104890934E-5	0.0	0.015564841566481125	3.7336951283912747E-4
27	2.3335594552445466E-4	4.6671189104890934E-5	0.0	0.04466432797338062	3.967051073915729E-4
28	2.3335594552445466E-4	4.6671189104890934E-5	0.0	0.12052834586338083	3.967051073915729E-4
29	2.3335594552445466E-4	4.6671189104890934E-5	0.0	0.24280686131819507	3.967051073915729E-4
30	2.3335594552445466E-4	4.6671189104890934E-5	0.0	0.3890510323783708	3.967051073915729E-4
31	2.3335594552445466E-4	4.6671189104890934E-5	0.0	0.744965520492269	4.2004070194401837E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	3655	0.0	14.830369	37
GGTATCA	12925	0.0	14.19884	1
ATACCCG	310	0.0	13.129032	5
CGAACCG	130	7.006496E-5	12.807693	18
TATACAC	1720	0.0	12.69186	37
TCTTATA	5580	0.0	12.664875	37
CGCGAAC	120	5.1265035E-4	12.333334	12
ATACCGT	195	1.4703437E-7	12.333334	6
CTCTTAT	8290	0.0	12.006032	37
ACCGACC	330	0.0	11.772727	8
TCGTTAC	490	0.0	11.7040825	23
TCTATAC	510	0.0	11.245098	3
GGGTAAG	1390	0.0	11.179856	1
TAAGGCG	285	1.4224497E-9	11.035088	5
GTATCAA	16835	0.0	10.912087	2
TCTCTTA	11810	0.0	10.730313	37
ACCCGTT	400	0.0	10.637499	7
GTCATAC	405	0.0	10.506173	1
TACCCGT	370	9.094947E-12	10.500001	6
CGACCGA	325	1.1350494E-9	10.246154	18
>>END_MODULE