FastQCFastQC Report
Thu 9 Feb 2017
SRR2727122.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727122.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044281
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT261980.8605644485512343No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163710.5377624470277218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT112360.36908550820374336No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG99550.32700660681454835No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC98240.3227034560870038No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA72020.2365747445784407No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65460.21502614246188181No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG57520.18894445026592485No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT51410.16887402969699578No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG48740.16010348584772563No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG48180.15826397103289744No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39660.13027706706443984No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC37770.12406870456439469No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35470.1165135544320646No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG35210.11565949398232292No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC34930.11473973657490882No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT32720.10748022275210468No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA32650.10725028340025115No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31180.10242155701132714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTAG8550.018.1754381
TTACGGT1054.8036964E-717.6190474
CAAGACG9250.017.44
GGTTATC4050.017.3580252
AAGACGG8850.017.3502835
TAGAGTC9400.016.9255335
CGCCCGA8500.016.54117826
TTAGAGT4350.016.1609214
GTATTAG7700.016.0974041
CTAGAGT10250.016.0634164
GACGGAC9750.015.9384617
TAGAGTG4550.015.8571425
TTATCTA4900.015.4795924
GCGCGTC1201.937955E-615.41666711
CACTCTA5950.015.2352949
ACGGACC10200.015.2352948
AGGTTAT4500.015.2111111
GCCCGAC9250.015.027
ACCGTCG7100.014.8521138
ATCTAGA10600.014.8349062
Produced by FastQC (version 0.11.5)