##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2727122.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3044281 Sequences flagged as poor quality 0 Sequence length 43 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.502995945512257 31.0 30.0 31.0 28.0 34.0 2 30.902977747454983 31.0 31.0 34.0 28.0 34.0 3 31.234277321968637 31.0 31.0 34.0 28.0 34.0 4 35.12866486372316 35.0 35.0 37.0 33.0 37.0 5 34.857648489084944 35.0 35.0 37.0 32.0 37.0 6 34.758363633317686 35.0 35.0 37.0 32.0 37.0 7 34.60490835110162 35.0 35.0 37.0 30.0 37.0 8 34.61077081911952 35.0 35.0 37.0 30.0 37.0 9 35.97239840868829 37.0 35.0 39.0 30.0 39.0 10 35.82581897006222 37.0 35.0 39.0 30.0 39.0 11 35.80786727637823 37.0 35.0 39.0 30.0 39.0 12 35.64210859641406 37.0 35.0 39.0 30.0 39.0 13 35.754765410945964 37.0 35.0 39.0 30.0 39.0 14 36.510391780522234 38.0 35.0 40.0 30.0 41.0 15 36.50882950686878 38.0 35.0 40.0 30.0 41.0 16 36.34022943348528 38.0 35.0 40.0 30.0 41.0 17 36.331390236315244 38.0 35.0 40.0 30.0 41.0 18 36.207120499060366 38.0 34.0 40.0 30.0 41.0 19 36.135149153445425 38.0 34.0 40.0 29.0 41.0 20 36.61129212447865 38.0 35.0 40.0 30.0 41.0 21 36.69520586305929 39.0 35.0 40.0 30.0 41.0 22 36.683961500268865 39.0 35.0 40.0 30.0 41.0 23 36.60084335184564 38.0 35.0 40.0 30.0 41.0 24 36.4465349946342 38.0 35.0 40.0 30.0 41.0 25 36.146689481030165 38.0 35.0 40.0 29.0 41.0 26 35.933731150311026 38.0 34.0 40.0 27.0 41.0 27 35.770405885659045 38.0 34.0 40.0 27.0 41.0 28 35.504797684576424 38.0 34.0 40.0 27.0 41.0 29 35.33225119494554 38.0 34.0 40.0 26.0 41.0 30 35.01764784525476 38.0 33.0 40.0 25.0 41.0 31 34.86078814669211 38.0 33.0 40.0 25.0 41.0 32 34.64148743167927 38.0 33.0 40.0 25.0 41.0 33 34.5721728710326 37.0 33.0 40.0 24.0 41.0 34 34.37120193569516 37.0 33.0 40.0 24.0 41.0 35 33.968362973063265 37.0 33.0 40.0 23.0 41.0 36 33.95302897465773 37.0 32.0 40.0 23.0 41.0 37 33.785911681608894 37.0 32.0 40.0 22.0 41.0 38 33.651319966849314 37.0 32.0 40.0 21.0 41.0 39 33.52277861340658 37.0 32.0 40.0 20.0 41.0 40 33.27148939273346 37.0 31.0 40.0 19.0 41.0 41 33.02652448969067 37.0 31.0 40.0 18.0 41.0 42 32.8195649481766 36.0 31.0 40.0 15.0 41.0 43 32.634075172429874 36.0 30.0 40.0 15.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 5.0 10 16.0 11 20.0 12 24.0 13 37.0 14 74.0 15 144.0 16 279.0 17 545.0 18 1022.0 19 2036.0 20 3750.0 21 6661.0 22 10934.0 23 16803.0 24 24543.0 25 34372.0 26 45690.0 27 60195.0 28 77225.0 29 96802.0 30 118323.0 31 144212.0 32 173827.0 33 207796.0 34 246376.0 35 287810.0 36 340516.0 37 395477.0 38 448191.0 39 300576.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.783660903839035 22.937961377415554 14.998057012476837 25.280320706268572 2 18.388151422289862 25.719406322872295 31.45767424229235 24.43476801254549 3 19.862029819192117 26.566141561833483 30.501356477933538 23.070472141040856 4 15.745524148394974 17.774344746756295 32.02894213773301 34.45118896711571 5 13.137289231841606 40.89343263647475 32.214371800763466 13.754906330920175 6 33.42868151790193 37.15980883499256 14.758460207845465 14.653049439260041 7 27.40098565145596 33.19240240963302 20.67995694221394 18.726654996697086 8 28.545295260194443 35.38287037234736 19.349363609995265 16.72247075746293 9 28.526177445511763 14.303278836611993 18.826777160189877 38.343766557686365 10 17.64357495250931 29.51892417289994 31.260189187529008 21.57731168706174 11 35.533152163023054 23.493757639324357 19.65574137210067 21.317348825551914 12 21.180206426410702 29.158871996376156 28.456078791675278 21.204842785537867 13 30.35504935319703 20.479022797172796 25.374135961824813 23.791791887805363 14 22.74215159507286 22.30920864401151 25.545802112222887 29.40283764869275 15 26.246263074926397 28.514253447694216 21.768227046057838 23.47125643132155 16 23.413705896400497 26.842364420367236 25.706792506999186 24.037137176233074 17 23.547037871996704 28.613061672033563 25.18735951116208 22.652540944807658 18 23.96220979600766 25.132568248463265 27.225837562301248 23.679384393227824 19 24.795772794955525 27.03456087003795 26.32759590852487 21.842070426481655 20 23.837385576430034 25.551090717315518 27.22718434993353 23.38433935632092 21 24.188864299977563 27.146836970700143 26.557239624068867 22.107059105253423 22 24.152730973257725 26.760276071755527 25.07137153239139 24.015621422595352 23 22.606158892690917 26.589496830286034 26.34618814754617 24.458156129476876 24 22.737125777810917 28.27514937024539 25.935582162093446 23.052142689850246 25 23.626925372526387 26.95776112651887 25.080667651902044 24.3346458490527 26 23.5849450165737 27.138329214681562 26.115558977637082 23.161166791107654 27 24.28156270725337 27.191445204959724 25.092361710367733 23.434630377419165 28 22.973634825431684 27.60724125006857 26.35486014595893 23.064263778540813 29 23.309839006320377 27.159877816798122 26.61091403848725 22.919369138394256 30 23.06019056716512 28.223215925205324 26.578426892918227 22.13816661471132 31 23.954818888269514 27.24741901289664 25.34670748199657 23.45105461683728 32 21.736823900290414 28.338579782878128 25.898791865796884 24.02580445103458 33 22.49871151841765 27.363768324934522 26.58903695158233 23.548483205065498 34 22.48281285466092 27.61939518723797 26.718032927972157 23.179759030128952 35 23.017487544678037 28.128776548551198 26.654044091199204 22.19969181557156 36 23.11570449639833 27.801835638694328 25.98285112313876 23.09960874176858 37 22.62829876742653 27.819869453575407 26.48871769721652 23.063114081781542 38 22.993015427945053 27.063598925329167 26.941763917325634 23.001621729400142 39 23.085451047390173 26.283743189278518 26.726277896160045 23.904527867171264 40 22.969725856450175 27.075785710977403 27.168878299999243 22.785610132573176 41 21.786556497248448 27.435148069445624 27.405091711310487 23.373203721995438 42 22.921537137997444 27.66538305760868 27.294655125463123 22.118424678930758 43 22.38640256927662 26.358933357334625 27.36031923465672 23.894344838732035 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 820.0 1 1112.5 2 1405.0 3 1421.5 4 1438.0 5 1438.0 6 1833.5 7 2229.0 8 3439.5 9 4650.0 10 7809.0 11 10968.0 12 10968.0 13 23730.5 14 36493.0 15 46299.5 16 56106.0 17 43632.0 18 31158.0 19 31158.0 20 35728.5 21 40299.0 22 36487.0 23 32675.0 24 36287.0 25 39899.0 26 39899.0 27 44854.0 28 49809.0 29 53586.0 30 57363.0 31 62395.0 32 67427.0 33 67427.0 34 77954.5 35 88482.0 36 102324.0 37 116166.0 38 130939.0 39 145712.0 40 145712.0 41 155235.5 42 164759.0 43 176569.0 44 188379.0 45 210510.5 46 232642.0 47 232642.0 48 279371.0 49 326100.0 50 317576.0 51 309052.0 52 265024.0 53 220996.0 54 220996.0 55 204573.5 56 188151.0 57 178712.0 58 169273.0 59 155954.5 60 142636.0 61 142636.0 62 121398.5 63 100161.0 64 86454.5 65 72748.0 66 61587.0 67 50426.0 68 50426.0 69 41139.0 70 31852.0 71 28563.5 72 25275.0 73 20146.0 74 15017.0 75 15017.0 76 11472.0 77 7927.0 78 6901.5 79 5876.0 80 4578.0 81 3280.0 82 3280.0 83 2817.5 84 2355.0 85 1916.5 86 1478.0 87 1467.0 88 1456.0 89 1456.0 90 1023.5 91 591.0 92 458.5 93 326.0 94 322.0 95 318.0 96 318.0 97 184.0 98 50.0 99 54.0 100 58.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 3044281.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.165462153951594 #Duplication Level Percentage of deduplicated Percentage of total 1 84.77111073216473 44.221241686471785 2 7.915662466757162 8.258483816661515 3 2.6259188987068236 4.109468187895112 4 1.2616800337321845 2.6326448820021056 5 0.7586394636278905 1.978738911418743 6 0.4904310845182242 1.535013850713412 7 0.36056381519845687 1.316628463907365 8 0.27659194789201763 1.154283743187904 9 0.20133852556574106 0.9452625511978872 >10 1.1633108587329952 11.091567603768157 >50 0.08949985499187821 3.2352946333412276 >100 0.06918947003167483 7.457910927187937 >500 0.009422694346427195 3.3351429918786097 >1k 0.006070997699644447 5.487952754280466 >5k 3.7943735622777795E-4 1.4653806375792775 >10k+ 1.8971867811388898E-4 1.7749843585086227 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 26198 0.8605644485512343 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16371 0.5377624470277218 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 11236 0.36908550820374336 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG 9955 0.32700660681454835 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 9824 0.3227034560870038 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA 7202 0.2365747445784407 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 6546 0.21502614246188181 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 5752 0.18894445026592485 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 5141 0.16887402969699578 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG 4874 0.16010348584772563 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 4818 0.15826397103289744 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3966 0.13027706706443984 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC 3777 0.12406870456439469 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3547 0.1165135544320646 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 3521 0.11565949398232292 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 3493 0.11473973657490882 No Hit GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT 3272 0.10748022275210468 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA 3265 0.10725028340025115 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3118 0.10242155701132714 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.2848478836217816E-5 2 0.0 0.0 0.0 6.569695767243563E-5 3.2848478836217816E-5 3 0.0 0.0 0.0 9.854543650865344E-5 3.2848478836217816E-5 4 0.0 0.0 0.0 1.3139391534487126E-4 6.569695767243563E-5 5 0.0 0.0 0.0 1.3139391534487126E-4 6.569695767243563E-5 6 0.0 0.0 0.0 1.3139391534487126E-4 6.569695767243563E-5 7 0.0 0.0 0.0 1.3139391534487126E-4 9.854543650865344E-5 8 0.0 0.0 0.0 1.3139391534487126E-4 9.854543650865344E-5 9 0.0 0.0 0.0 1.3139391534487126E-4 9.854543650865344E-5 10 0.0 0.0 0.0 1.3139391534487126E-4 9.854543650865344E-5 11 0.0 0.0 0.0 1.6424239418108907E-4 9.854543650865344E-5 12 0.0 0.0 0.0 1.9709087301730688E-4 9.854543650865344E-5 13 0.0 0.0 0.0 2.6278783068974253E-4 9.854543650865344E-5 14 0.0 0.0 0.0 4.927271825432672E-4 9.854543650865344E-5 15 0.0 3.2848478836217816E-5 0.0 6.898180555605741E-4 9.854543650865344E-5 16 0.0 3.2848478836217816E-5 0.0 0.001576726984138455 1.3139391534487126E-4 17 0.0 3.2848478836217816E-5 0.0 0.0021351511243541577 1.3139391534487126E-4 18 0.0 3.2848478836217816E-5 0.0 0.002792120701078514 1.6424239418108907E-4 19 0.0 3.2848478836217816E-5 0.0 0.003974665939182356 1.6424239418108907E-4 20 0.0 3.2848478836217816E-5 0.0 0.0051900596561224146 1.6424239418108907E-4 21 0.0 3.2848478836217816E-5 0.0 0.009131877116468552 1.9709087301730688E-4 22 0.0 3.2848478836217816E-5 0.0 0.014913209391642887 2.299393518535247E-4 23 0.0 3.2848478836217816E-5 0.0 0.028151146362638664 3.2848478836217815E-4 24 0.0 3.2848478836217816E-5 0.0 0.053083141799327986 3.2848478836217815E-4 25 0.0 3.2848478836217816E-5 0.0 0.06885041164071254 3.2848478836217815E-4 26 0.0 3.2848478836217816E-5 0.0 0.08524180257998522 3.2848478836217815E-4 27 0.0 3.2848478836217816E-5 0.0 0.11181622195848544 3.2848478836217815E-4 28 0.0 3.2848478836217816E-5 0.0 0.16128603108582945 3.2848478836217815E-4 29 0.0 3.2848478836217816E-5 0.0 0.21496044550420937 3.2848478836217815E-4 30 0.0 3.2848478836217816E-5 0.0 0.29369824927462346 3.2848478836217815E-4 31 0.0 3.2848478836217816E-5 0.0 0.4786351851225297 3.9418174603461376E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCTAG 855 0.0 18.175438 1 TTACGGT 105 4.8036964E-7 17.619047 4 CAAGACG 925 0.0 17.4 4 GGTTATC 405 0.0 17.358025 2 AAGACGG 885 0.0 17.350283 5 TAGAGTC 940 0.0 16.925533 5 CGCCCGA 850 0.0 16.541178 26 TTAGAGT 435 0.0 16.160921 4 GTATTAG 770 0.0 16.097404 1 CTAGAGT 1025 0.0 16.063416 4 GACGGAC 975 0.0 15.938461 7 TAGAGTG 455 0.0 15.857142 5 TTATCTA 490 0.0 15.479592 4 GCGCGTC 120 1.937955E-6 15.416667 11 CACTCTA 595 0.0 15.235294 9 ACGGACC 1020 0.0 15.235294 8 AGGTTAT 450 0.0 15.211111 1 GCCCGAC 925 0.0 15.0 27 ACCGTCG 710 0.0 14.852113 8 ATCTAGA 1060 0.0 14.834906 2 >>END_MODULE