FastQCFastQC Report
Thu 9 Feb 2017
SRR2727121.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727121.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3357361
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT256630.764380118789728No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158600.472394836301488No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT113110.33690151282510283No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC86660.25811939794380173No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG82970.24712862274864095No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT67360.20063377158428897No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA61770.18398378964907258No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG53140.15827907692976714No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT48700.14505440433721606No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG43880.1306978903966538No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41500.1236089893222683No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG40440.12045174766728986No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT39500.11765192959589392No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC34520.10281885087722173No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG33920.10103173295930941No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33910.10100194766067754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTTAT5550.020.6666661
GGTTATC6250.018.6482
AATACCG953.6105648E-617.5263165
GTTATCT7200.016.9583343
TACCGTC8050.016.5465857
CGAACGA4200.016.29761916
CCGTCGT8000.016.18759
CGTCGTA7800.016.12820410
ATACCGT8650.016.0404626
ACGAACG4400.015.97727315
TTATCTA7350.015.8571424
ACCGTCG8250.015.696978
AAGACGG10200.015.4166675
TAGAGTG5400.015.0740735
GTAATAC2600.014.9423073
CGTTAAT1001.0943575E-414.81
GTATTAG9400.014.563831
TTAGACT2550.014.5098044
GTCGTAG8700.014.4597711
TATTCCG1051.6571271E-414.0952385
Produced by FastQC (version 0.11.5)