FastQCFastQC Report
Thu 9 Feb 2017
SRR2727120.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727120.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5453432
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT220790.4048643129684206No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129050.23663997277310875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT117700.215827390898062No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT73230.13428241151627085No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT70150.12863459194136828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT4800.019.2708324
AAGACGG17950.016.7994425
ACGAACG8750.016.70285815
CGAACGA8850.016.51412616
CACTCTA11250.016.4444459
CGCTTCG10850.016.36866432
TCGAACG5550.016.3333343
CAAGACG18850.016.2917774
TATCTAG16450.016.1945291
AGGTTAT8750.015.6457151
GTAAACG11400.015.57894727
TTCGGGC11750.015.11489435
TAGAGTG10350.014.8357495
TAAACGC12350.014.82995928
CGATAAC10000.014.79999910
CTAGAGT21350.014.6440284
TAACGAA10250.014.61951213
CCGATAA10450.014.5167479
GTATTAG13850.014.4259931
GGTTATC8850.014.423732
Produced by FastQC (version 0.11.5)