##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727120.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5453432
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.638636366970378	31.0	31.0	31.0	28.0	34.0
2	31.05659408607277	31.0	31.0	34.0	28.0	34.0
3	31.379588670033844	31.0	31.0	34.0	30.0	34.0
4	35.26172582696548	35.0	35.0	37.0	33.0	37.0
5	35.010043033451225	35.0	35.0	37.0	32.0	37.0
6	34.86055808525714	35.0	35.0	37.0	32.0	37.0
7	34.76510480005985	35.0	35.0	37.0	32.0	37.0
8	34.73987499981663	35.0	35.0	37.0	32.0	37.0
9	36.137495434067944	37.0	35.0	39.0	32.0	39.0
10	35.98634034494241	37.0	35.0	39.0	30.0	39.0
11	35.951801911163464	37.0	35.0	39.0	30.0	39.0
12	35.77106141600372	37.0	35.0	39.0	30.0	39.0
13	35.86753735995975	37.0	35.0	39.0	30.0	39.0
14	36.690493252689315	38.0	36.0	40.0	31.0	41.0
15	36.65810814180868	38.0	36.0	40.0	31.0	41.0
16	36.523149825651075	38.0	35.0	40.0	30.0	41.0
17	36.418624821946985	38.0	35.0	40.0	30.0	41.0
18	36.27425775181574	38.0	35.0	40.0	30.0	41.0
19	36.1955117437973	38.0	34.0	40.0	29.0	41.0
20	36.64087862468992	38.0	35.0	40.0	30.0	41.0
21	36.72348055316358	39.0	35.0	40.0	30.0	41.0
22	36.67825820510827	39.0	35.0	40.0	30.0	41.0
23	36.59703100726295	38.0	35.0	40.0	30.0	41.0
24	36.420595874304475	38.0	35.0	40.0	30.0	41.0
25	36.100722077400064	38.0	34.0	40.0	29.0	41.0
26	35.91283929092725	38.0	34.0	40.0	27.0	41.0
27	35.74448970849916	38.0	34.0	40.0	27.0	41.0
28	35.47455858989348	38.0	34.0	40.0	27.0	41.0
29	35.29161984599789	38.0	34.0	40.0	26.0	41.0
30	35.0101015287254	38.0	34.0	40.0	25.0	41.0
31	34.867598972536925	38.0	33.0	40.0	25.0	41.0
32	34.68957804919911	38.0	33.0	40.0	24.0	41.0
33	34.59855610191894	38.0	33.0	40.0	24.0	41.0
34	34.41200550405689	38.0	33.0	40.0	24.0	41.0
35	33.990486357948534	37.0	32.0	40.0	22.0	41.0
36	33.95532006266879	37.0	32.0	40.0	22.0	41.0
37	33.81383759804835	37.0	32.0	40.0	21.0	41.0
38	33.68879267221082	37.0	32.0	40.0	21.0	41.0
39	33.56485695613331	37.0	31.0	40.0	20.0	41.0
40	33.35409591611301	37.0	31.0	40.0	18.0	41.0
41	33.13842329014096	37.0	31.0	40.0	18.0	41.0
42	32.969574755860165	37.0	31.0	40.0	16.0	41.0
43	32.848776146837444	37.0	30.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	28.0
11	50.0
12	54.0
13	68.0
14	146.0
15	317.0
16	560.0
17	987.0
18	1814.0
19	3586.0
20	6885.0
21	12299.0
22	20089.0
23	31164.0
24	45113.0
25	62203.0
26	83480.0
27	107821.0
28	136571.0
29	168803.0
30	207604.0
31	250075.0
32	300869.0
33	358972.0
34	425699.0
35	501913.0
36	596103.0
37	696558.0
38	828382.0
39	605211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.144541639099934	22.907116105967766	14.867463278170517	26.080878976761788
2	19.227800034913795	23.880008038974356	32.650650086037565	24.241541840074287
3	18.847012303444878	25.614512109071864	30.471050157038725	25.067425430444533
4	15.185574882019248	17.18739685394445	33.72683477120463	33.90019349283167
5	13.110808019610404	39.98654425323356	33.28780848463867	13.614839242517373
6	33.65238624044455	38.5841246393097	14.328664958140122	13.434824162105624
7	28.46348868015591	32.60687948433207	20.75093262371292	18.178699211799103
8	27.45337248176928	36.43551436966666	18.75017420222715	17.360938946336912
9	27.816098192844436	14.661684605217411	18.395681838519305	39.12653536341885
10	16.806865841547122	28.62307625729999	32.19563386872707	22.374424032425818
11	36.936556649097305	22.83717849603699	20.131451166898202	20.094813687967502
12	21.238717196803776	27.44748628019933	28.934733210205977	22.37906331279092
13	30.064443821798825	20.502685281488795	24.753806410348567	24.679064486363817
14	22.557776460768192	21.62674807350674	24.627573975434185	31.187901490290887
15	25.381997978520683	29.03160798557679	21.162691677461094	24.42370235844144
16	24.674608576764136	26.886793490777915	23.84624948106073	24.59234845139721
17	23.893485790232646	27.763764176393874	24.391264070038833	23.951485963334648
18	24.52688508814266	25.538009825739096	25.627531433416607	24.307573652701638
19	25.297940819652652	26.01079467021868	25.15522335292711	23.536041157201556
20	24.52736185213275	25.23548473695097	25.557538812256208	24.679614598660073
21	25.24531340997742	26.13952094754276	25.189770405132034	23.425395237347786
22	24.837900243369678	25.68861223537765	24.892453046081805	24.581034475170867
23	23.856224850699522	25.559189149144977	25.378678967666602	25.2059070324889
24	24.114539247945146	26.29107688516149	25.219549817436064	24.374834049457295
25	24.621137661567982	25.683514528099003	24.919078481220634	24.77626932911238
26	24.685464126076937	26.10480886164896	25.118879267221082	24.090847745053022
27	25.15617688090729	26.019394759116825	24.51632293205453	24.308105427921355
28	24.27986633004684	26.14183141918704	25.29214630346541	24.28615594730071
29	24.278179319004987	26.269365786535893	25.49460596556444	23.957848928894684
30	24.354168164194583	26.375849190014655	25.388397618233803	23.88158502755696
31	24.82246042492141	25.788255909306283	25.022279547998398	24.36700411777391
32	23.761440502054487	26.25830852938113	24.95074661240848	25.0295043561559
33	23.928729651346163	25.54739840892854	25.5113110422941	25.012560897431197
34	24.414772202165537	25.829110915841618	25.80846336765545	23.947653514337393
35	24.932757940320887	25.723012590970235	25.549470498577776	23.794758970131102
36	24.235288896973504	26.02456581470164	25.49387248250276	24.246272805822095
37	24.379344970286603	25.670091788070337	25.635471387559246	24.315091854083814
38	24.224836763344623	24.96074031912381	26.221157612307262	24.593265305224307
39	24.4355481098875	24.955862656763667	25.920099489642485	24.68848974370635
40	24.471287072067643	25.07380673308111	26.37628927985166	24.078616914999582
41	23.42205788941716	25.5725935520971	26.7200544537825	24.28529410470324
42	24.339846907415367	25.40594620048439	26.67736573959297	23.576841152507267
43	23.618539664563524	24.704571359833587	26.641168350499285	25.035720625103604
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1040.0
1	1226.5
2	1413.0
3	1356.0
4	1299.0
5	1299.0
6	1679.5
7	2060.0
8	2955.5
9	3851.0
10	6406.5
11	8962.0
12	8962.0
13	19740.5
14	30519.0
15	38978.5
16	47438.0
17	39142.5
18	30847.0
19	30847.0
20	37323.5
21	43800.0
22	42612.0
23	41424.0
24	48892.0
25	56360.0
26	56360.0
27	66300.5
28	76241.0
29	86839.0
30	97437.0
31	110161.5
32	122886.0
33	122886.0
34	144856.5
35	166827.0
36	195161.0
37	223495.0
38	252970.5
39	282446.0
40	282446.0
41	301275.5
42	320105.0
43	338993.0
44	357881.0
45	388677.0
46	419473.0
47	419473.0
48	459044.0
49	498615.0
50	493364.5
51	488114.0
52	459583.5
53	431053.0
54	431053.0
55	409521.0
56	387989.0
57	368582.0
58	349175.0
59	322592.5
60	296010.0
61	296010.0
62	252651.0
63	209292.0
64	181441.5
65	153591.0
66	129581.0
67	105571.0
68	105571.0
69	86246.0
70	66921.0
71	59536.0
72	52151.0
73	41614.5
74	31078.0
75	31078.0
76	23862.0
77	16646.0
78	14191.0
79	11736.0
80	9149.5
81	6563.0
82	6563.0
83	5596.0
84	4629.0
85	3759.5
86	2890.0
87	2914.0
88	2938.0
89	2938.0
90	2072.5
91	1207.0
92	931.0
93	655.0
94	614.5
95	574.0
96	574.0
97	342.5
98	111.0
99	115.0
100	119.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5453432.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.761826662353386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.2171145359676	43.07469857951963
2	8.597600359735614	8.900549990656438
3	2.896796225840834	4.498303923543983
4	1.4215305533370732	2.9432407238829152
5	0.8162853986931569	2.1126211657082608
6	0.5560283733429526	1.7268626568196936
7	0.39157490868302625	1.4188042784004362
8	0.30303158588284734	1.25483747373488
9	0.2278337871193931	1.0613783697031391
>10	1.3931344970490744	13.486724805788702
>50	0.10299647382209579	3.6517410322566763
>100	0.06204243609344634	6.233895622884289
>500	0.008089226724966725	2.927989913268562
>1k	0.005726879718464784	5.483357497154782
>5k	1.0737899472088995E-4	0.35877747946446603
>10k+	1.0737899472088995E-4	0.8662164872130981
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	22079	0.4048643129684206	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12905	0.23663997277310875	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11770	0.215827390898062	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7323	0.13428241151627085	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	7015	0.12863459194136828	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8337076541891417E-5
2	0.0	0.0	0.0	0.0	1.8337076541891417E-5
3	0.0	0.0	0.0	0.0	1.8337076541891417E-5
4	0.0	0.0	0.0	0.0	1.8337076541891417E-5
5	0.0	0.0	0.0	0.0	1.8337076541891417E-5
6	1.8337076541891417E-5	0.0	0.0	0.0	3.6674153083782834E-5
7	1.8337076541891417E-5	0.0	0.0	0.0	3.6674153083782834E-5
8	1.8337076541891417E-5	0.0	0.0	0.0	3.6674153083782834E-5
9	1.8337076541891417E-5	0.0	0.0	0.0	9.168538270945709E-5
10	1.8337076541891417E-5	0.0	0.0	0.0	9.168538270945709E-5
11	1.8337076541891417E-5	0.0	0.0	0.0	9.168538270945709E-5
12	1.8337076541891417E-5	0.0	0.0	0.0	9.168538270945709E-5
13	1.8337076541891417E-5	1.8337076541891417E-5	0.0	1.8337076541891417E-5	9.168538270945709E-5
14	1.8337076541891417E-5	3.6674153083782834E-5	0.0	1.8337076541891417E-5	1.4669661233513134E-4
15	1.8337076541891417E-5	3.6674153083782834E-5	0.0	1.6503368887702276E-4	1.4669661233513134E-4
16	1.8337076541891417E-5	3.6674153083782834E-5	0.0	2.933932246702627E-4	1.8337076541891418E-4
17	3.6674153083782834E-5	3.6674153083782834E-5	0.0	5.134381431729597E-4	1.8337076541891418E-4
18	3.6674153083782834E-5	3.6674153083782834E-5	0.0	6.417976789661996E-4	2.2004491850269702E-4
19	3.6674153083782834E-5	3.6674153083782834E-5	0.0	9.351909036364623E-4	2.3838199504458844E-4
20	3.6674153083782834E-5	3.6674153083782834E-5	0.0	0.0013019324344742907	2.3838199504458844E-4
21	3.6674153083782834E-5	3.6674153083782834E-5	0.0	0.00245716825661345	2.5671907158647983E-4
22	3.6674153083782834E-5	3.6674153083782834E-5	0.0	0.004290875910802592	2.5671907158647983E-4
23	3.6674153083782834E-5	3.6674153083782834E-5	0.0	0.0077932575303038525	3.6674153083782836E-4
24	5.5011229625674255E-5	3.6674153083782834E-5	0.0	0.013551099564457759	3.6674153083782836E-4
25	5.5011229625674255E-5	3.6674153083782834E-5	0.0	0.01604494197415499	3.6674153083782836E-4
26	7.334830616756567E-5	3.6674153083782834E-5	0.0	0.02000575050720354	3.6674153083782836E-4
27	7.334830616756567E-5	3.6674153083782834E-5	0.0	0.028770873094227635	3.6674153083782836E-4
28	7.334830616756567E-5	3.6674153083782834E-5	0.0	0.04965680327544196	3.6674153083782836E-4
29	7.334830616756567E-5	3.6674153083782834E-5	0.0	0.07848268759929528	3.6674153083782836E-4
30	7.334830616756567E-5	3.6674153083782834E-5	0.0	0.12289508698375629	3.6674153083782836E-4
31	7.334830616756567E-5	3.6674153083782834E-5	0.0	0.2595796555270149	3.6674153083782836E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	480	0.0	19.270832	4
AAGACGG	1795	0.0	16.799442	5
ACGAACG	875	0.0	16.702858	15
CGAACGA	885	0.0	16.514126	16
CACTCTA	1125	0.0	16.444445	9
CGCTTCG	1085	0.0	16.368664	32
TCGAACG	555	0.0	16.333334	3
CAAGACG	1885	0.0	16.291777	4
TATCTAG	1645	0.0	16.194529	1
AGGTTAT	875	0.0	15.645715	1
GTAAACG	1140	0.0	15.578947	27
TTCGGGC	1175	0.0	15.114894	35
TAGAGTG	1035	0.0	14.835749	5
TAAACGC	1235	0.0	14.829959	28
CGATAAC	1000	0.0	14.799999	10
CTAGAGT	2135	0.0	14.644028	4
TAACGAA	1025	0.0	14.619512	13
CCGATAA	1045	0.0	14.516747	9
GTATTAG	1385	0.0	14.425993	1
GGTTATC	885	0.0	14.42373	2
>>END_MODULE