FastQCFastQC Report
Thu 9 Feb 2017
SRR2727119.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727119.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817213
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT284651.0103957350757646No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166880.5923584762671477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT115350.4094472089969768No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG70660.25081525607044974No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC70460.25010533459841344No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62960.22348327939704948No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA50200.17819028948112905No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG45160.1603002683858125No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43590.154727384830327No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT42690.15153273820616334No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG38290.13591446582136316No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36650.13009310975066493No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG33940.12047367380457209No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT32080.11387140411463385No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC28230.10020541577793372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTTAT4300.019.3604641
GGTTATC5000.016.2799992
ATACCGT7100.015.6338036
TACCGTC6850.015.3941617
GTTATCT5550.015.3333333
CGTCGTA6750.015.07407510
ACGAACG3950.014.98734215
TAATACC3100.014.9193544
TATCTAG7600.014.8486841
TATACCG1001.0942584E-414.85
TTATCTA5650.014.7345134
CGAACGA3900.014.70512916
ACCGTCG6900.014.478268
AAGACGG8500.014.3647065
CCGTCGT7100.014.3309869
ACTGCGC1551.212029E-714.322588
TAGAGTC9250.014.25
ATAACGA4050.014.16049412
CTAGAGT9500.014.0210524
CGAACGT2252.7284841E-1113.97777754
Produced by FastQC (version 0.11.5)