##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727119.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2817213
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.45592221816384	31.0	30.0	31.0	28.0	34.0
2	30.864234617687764	31.0	31.0	34.0	28.0	34.0
3	31.18522632119048	31.0	31.0	34.0	28.0	34.0
4	35.08864044003773	35.0	35.0	37.0	33.0	37.0
5	34.806448074746214	35.0	35.0	37.0	32.0	37.0
6	34.694552737048994	35.0	35.0	37.0	32.0	37.0
7	34.54022716777184	35.0	35.0	37.0	30.0	37.0
8	34.546756315550155	35.0	35.0	37.0	30.0	37.0
9	35.88366800806329	37.0	35.0	39.0	30.0	39.0
10	35.73049499629599	37.0	35.0	39.0	30.0	39.0
11	35.71699335478006	37.0	35.0	39.0	30.0	39.0
12	35.53853329513956	37.0	35.0	39.0	30.0	39.0
13	35.65642533951107	37.0	35.0	39.0	30.0	39.0
14	36.38892302427967	38.0	35.0	40.0	30.0	41.0
15	36.39368127294599	38.0	35.0	40.0	30.0	41.0
16	36.2222646991903	38.0	34.0	40.0	30.0	41.0
17	36.21786709063177	38.0	34.0	40.0	30.0	41.0
18	36.09681483082749	38.0	34.0	40.0	29.0	41.0
19	36.02550925329395	38.0	34.0	40.0	28.0	41.0
20	36.504943360690156	38.0	35.0	40.0	30.0	41.0
21	36.59277236048535	38.0	35.0	40.0	30.0	41.0
22	36.57642712851318	38.0	35.0	40.0	30.0	41.0
23	36.48356017099169	38.0	35.0	40.0	30.0	41.0
24	36.3285513022977	38.0	35.0	40.0	30.0	41.0
25	36.018374542499984	38.0	34.0	40.0	28.0	41.0
26	35.782678838980225	38.0	34.0	40.0	27.0	41.0
27	35.60484776976395	38.0	34.0	40.0	27.0	41.0
28	35.34233052310918	38.0	34.0	40.0	26.0	41.0
29	35.149718178923635	38.0	34.0	40.0	25.0	41.0
30	34.82877049055219	38.0	33.0	40.0	25.0	41.0
31	34.65576795222796	38.0	33.0	40.0	25.0	41.0
32	34.44048320095073	37.0	33.0	40.0	24.0	41.0
33	34.36191370691531	37.0	33.0	40.0	24.0	41.0
34	34.15935642778874	37.0	33.0	40.0	24.0	41.0
35	33.73868429543666	37.0	32.0	40.0	21.0	41.0
36	33.70245629279717	37.0	32.0	40.0	21.0	41.0
37	33.535985741937154	37.0	31.0	40.0	21.0	41.0
38	33.395236710891226	37.0	31.0	40.0	20.0	41.0
39	33.25565869531342	37.0	31.0	40.0	19.0	41.0
40	32.98538271689077	36.0	31.0	40.0	18.0	41.0
41	32.727646791350175	36.0	30.0	40.0	15.0	41.0
42	32.49875462025768	36.0	30.0	39.0	15.0	41.0
43	32.30070463255707	36.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	8.0
11	17.0
12	23.0
13	37.0
14	63.0
15	137.0
16	271.0
17	532.0
18	964.0
19	1955.0
20	3944.0
21	6785.0
22	11346.0
23	17333.0
24	24902.0
25	34867.0
26	45946.0
27	60278.0
28	75496.0
29	93751.0
30	115055.0
31	138019.0
32	164115.0
33	194772.0
34	229416.0
35	264929.0
36	311917.0
37	359313.0
38	397034.0
39	263981.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.32327410103532	23.486899996556883	15.320531319428103	24.869294582979702
2	18.63540314488113	25.924912315824184	31.18674377833696	24.252940760957728
3	19.889834385969397	26.378339160013812	30.601271540348563	23.130554913668224
4	15.84594420088222	17.701536944490883	31.816373131886017	34.63614572274088
5	13.171137574617184	40.9920016697353	31.98022300763201	13.856637748015505
6	33.50027136748269	37.50362503651659	14.406578416328477	14.58952517967225
7	27.513361609505566	33.19333681904776	20.43370522569646	18.859596345750216
8	28.794166433279983	35.42135436688671	18.967717385941356	16.816761813891958
9	28.365480352390822	14.262925806461919	18.880929485984908	38.49066435516235
10	17.655179072366913	29.81283275350497	31.008021047751804	21.523967126376316
11	35.65655135057236	23.60613130778539	19.379223367207235	21.35809397443502
12	21.077355528318233	29.500751274397786	28.27982832678963	21.14206487049435
13	30.420490037494503	20.451062805687748	25.37845026272419	23.74999689409356
14	22.754332029562548	22.371045426810113	25.503431937876194	29.371190605751146
15	26.089010664085393	28.808187382352703	21.70009864358854	23.402703309973365
16	23.413778084937135	26.901267316315806	25.467225942802337	24.217728655944722
17	23.429076892659516	28.819794598420494	25.191172978400996	22.55995553051899
18	23.798307050265635	25.22272898783301	27.261978416257488	23.71698554564387
19	24.61727246040679	26.94876106279504	26.51254981430229	21.921416662495876
20	23.9030559634646	25.669127609449482	27.225630436889226	23.202185990196693
21	24.31197073135755	27.148461972878867	26.490897209405183	22.048670086358396
22	24.132183118564342	26.75122541320092	25.397866614984384	23.718724853250357
23	22.61188628619845	26.682576006855	26.43250616833019	24.273031538616355
24	22.849745475404237	28.255868477108404	25.991715926342806	22.90267012114455
25	23.607444662508655	27.042470697103838	25.32634202667672	24.023742613710787
26	23.648549115739563	27.317742747885944	26.128446801857013	22.905261334517483
27	24.04514674609268	27.388415430427166	25.28048109958317	23.285956723896987
28	22.93916008480722	27.609165512156874	26.67118176722882	22.78049263580709
29	23.217023348962254	27.32058243377409	26.72765602032931	22.734738196934345
30	22.894648008510536	28.454398016763378	26.5654034678954	22.085550506830685
31	23.723765295701817	27.555317968502912	25.47350874782986	23.24740798796541
32	21.751319477795963	28.453084662040105	26.00623382044595	23.789362039717975
33	22.352693956757975	27.64082800980969	26.682292038266187	23.324185995166143
34	22.406754476853543	27.795661882860827	26.654072659752742	23.143510980532888
35	22.927304396224212	28.244190268893405	26.652830297176678	22.175675037705705
36	22.858442013436683	28.07494498995994	26.037186396626737	23.029426599976645
37	22.496027101962117	28.01942913084669	26.44365193544116	23.04089183175003
38	22.69618946100277	27.31383817978974	26.998633046205594	22.991339313001895
39	22.845805411234434	26.5595466157511	26.792542842873434	23.80210513014103
40	22.67194564273273	27.341773589714375	27.2446563323398	22.741624435213097
41	21.61678935884507	27.644022656433858	27.560180930586363	23.179007054134708
42	22.702294785662282	27.786716872313168	27.445209148190074	22.065779193834473
43	22.12133054902132	26.65875814146818	27.52422340802772	23.695687901482778
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	635.0
1	909.5
2	1184.0
3	1233.5
4	1283.0
5	1283.0
6	1682.5
7	2082.0
8	3130.0
9	4178.0
10	7323.5
11	10469.0
12	10469.0
13	23472.0
14	36475.0
15	47740.0
16	59005.0
17	44842.5
18	30680.0
19	30680.0
20	34948.5
21	39217.0
22	34826.0
23	30435.0
24	33835.5
25	37236.0
26	37236.0
27	41729.5
28	46223.0
29	49718.5
30	53214.0
31	57938.0
32	62662.0
33	62662.0
34	72507.0
35	82352.0
36	95213.0
37	108074.0
38	121639.0
39	135204.0
40	135204.0
41	144826.5
42	154449.0
43	164595.5
44	174742.0
45	195090.5
46	215439.0
47	215439.0
48	253144.0
49	290849.0
50	281417.0
51	271985.0
52	237428.0
53	202871.0
54	202871.0
55	189101.0
56	175331.0
57	166607.0
58	157883.0
59	145692.5
60	133502.0
61	133502.0
62	114014.0
63	94526.0
64	81392.5
65	68259.0
66	57717.5
67	47176.0
68	47176.0
69	38578.0
70	29980.0
71	26860.5
72	23741.0
73	18787.0
74	13833.0
75	13833.0
76	10667.0
77	7501.0
78	6495.5
79	5490.0
80	4281.5
81	3073.0
82	3073.0
83	2550.0
84	2027.0
85	1707.0
86	1387.0
87	1390.0
88	1393.0
89	1393.0
90	957.0
91	521.0
92	427.0
93	333.0
94	282.5
95	232.0
96	232.0
97	133.5
98	35.0
99	41.0
100	47.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2817213.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.23986694671208
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.19461489909229	45.35749961804048
2	7.728990889454495	8.229808931738143
3	2.4683330651502913	3.942411719063145
4	1.2255108027979662	2.609841283308881
5	0.7638298659942725	2.033310021772999
6	0.50266487883369	1.6057086760734487
7	0.3308741075705173	1.2330985424216505
8	0.26069807960799807	1.1103624857274537
9	0.20080514277106823	0.9621755175012476
>10	1.1575325304271824	11.135588799579251
>50	0.08463086260495882	3.128107301849714
>100	0.06588425560983623	7.263491387791386
>500	0.009618843129532994	3.4694821019803217
>1k	0.0055441943038150076	4.998829556471327
>5k	2.6719008693084376E-4	0.9042937185600258
>10k+	2.0039256519813282E-4	2.015990338120605
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28465	1.0103957350757646	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16688	0.5923584762671477	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11535	0.4094472089969768	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	7066	0.25081525607044974	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	7046	0.25010533459841344	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6296	0.22348327939704948	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	5020	0.17819028948112905	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	4516	0.1603002683858125	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4359	0.154727384830327	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	4269	0.15153273820616334	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	3829	0.13591446582136316	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3665	0.13009310975066493	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	3394	0.12047367380457209	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3208	0.11387140411463385	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	2823	0.10020541577793372	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.5496073601818535E-5
2	0.0	0.0	0.0	0.0	3.5496073601818535E-5
3	0.0	0.0	0.0	0.0	3.5496073601818535E-5
4	0.0	0.0	0.0	0.0	3.5496073601818535E-5
5	0.0	0.0	0.0	0.0	3.5496073601818535E-5
6	0.0	0.0	0.0	0.0	7.099214720363707E-5
7	0.0	0.0	0.0	0.0	1.064882208054556E-4
8	7.099214720363707E-5	0.0	0.0	0.0	1.064882208054556E-4
9	7.099214720363707E-5	0.0	0.0	0.0	1.4198429440727414E-4
10	1.064882208054556E-4	0.0	0.0	7.099214720363707E-5	1.4198429440727414E-4
11	1.064882208054556E-4	0.0	0.0	1.4198429440727414E-4	1.4198429440727414E-4
12	1.064882208054556E-4	0.0	0.0	2.839685888145483E-4	1.4198429440727414E-4
13	1.064882208054556E-4	0.0	0.0	4.259528832218224E-4	1.4198429440727414E-4
14	1.4198429440727414E-4	0.0	0.0	6.389293248327336E-4	1.7748036800909267E-4
15	1.4198429440727414E-4	0.0	0.0	0.0010293861344527375	1.7748036800909267E-4
16	1.4198429440727414E-4	0.0	0.0	0.0020232761953036566	1.7748036800909267E-4
17	1.4198429440727414E-4	0.0	0.0	0.0030171662561545754	1.7748036800909267E-4
18	1.4198429440727414E-4	0.0	0.0	0.003762583801792765	2.4847251521272975E-4
19	1.4198429440727414E-4	0.0	0.0	0.005430899261078236	2.4847251521272975E-4
20	1.4198429440727414E-4	0.0	0.0	0.0070282225731600695	2.839685888145483E-4
21	1.4198429440727414E-4	0.0	0.0	0.011926680730211027	2.839685888145483E-4
22	1.4198429440727414E-4	0.0	0.0	0.019203375818583827	2.839685888145483E-4
23	1.4198429440727414E-4	0.0	0.0	0.03148501728481304	3.5496073601818534E-4
24	1.4198429440727414E-4	0.0	0.0	0.056545245247696926	3.5496073601818534E-4
25	1.4198429440727414E-4	0.0	0.0	0.07045970609960979	3.5496073601818534E-4
26	1.4198429440727414E-4	0.0	0.0	0.08756881357568633	3.5496073601818534E-4
27	1.4198429440727414E-4	0.0	0.0	0.11174163969852474	3.5496073601818534E-4
28	1.4198429440727414E-4	0.0	0.0	0.1570346296144452	3.5496073601818534E-4
29	1.4198429440727414E-4	0.0	0.0	0.20814897560106388	3.5496073601818534E-4
30	1.4198429440727414E-4	0.0	0.0	0.2887960548243956	3.5496073601818534E-4
31	1.4198429440727414E-4	0.0	0.0	0.47241724356660286	3.5496073601818534E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTTAT	430	0.0	19.360464	1
GGTTATC	500	0.0	16.279999	2
ATACCGT	710	0.0	15.633803	6
TACCGTC	685	0.0	15.394161	7
GTTATCT	555	0.0	15.333333	3
CGTCGTA	675	0.0	15.074075	10
ACGAACG	395	0.0	14.987342	15
TAATACC	310	0.0	14.919354	4
TATCTAG	760	0.0	14.848684	1
TATACCG	100	1.0942584E-4	14.8	5
TTATCTA	565	0.0	14.734513	4
CGAACGA	390	0.0	14.705129	16
ACCGTCG	690	0.0	14.47826	8
AAGACGG	850	0.0	14.364706	5
CCGTCGT	710	0.0	14.330986	9
ACTGCGC	155	1.212029E-7	14.32258	8
TAGAGTC	925	0.0	14.2	5
ATAACGA	405	0.0	14.160494	12
CTAGAGT	950	0.0	14.021052	4
CGAACGT	225	2.7284841E-11	13.9777775	4
>>END_MODULE