FastQCFastQC Report
Thu 9 Feb 2017
SRR2727118.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727118.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2808321
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT391631.3945343142753268No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222440.7920746951648334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT155100.5522872919441901No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81170.2890339102972915No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG80190.28554428072859195No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC73590.2620426938373498No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA61130.2176745464638836No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58630.20877243021720096No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG56900.2026121657744966No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT51490.18334798621667536No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT49840.17747258949386482No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG48990.17444586996999276No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG46480.1655081452583234No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39310.1399768758628376No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC39060.13908666423816934No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA29340.1044752362710673No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28140.10020222047265963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGG5150.016.88349537
AAGACGG6800.016.3235285
CAAGACG7150.016.0419584
CGCTTCG4100.015.79268432
GGTTATC3750.015.7866662
ATGGTCG5400.015.75925936
TAGAGTG4250.015.67058855
TTAGAGT4450.015.3820234
GTAAACG4500.015.21111127
ATAACGC6800.014.9632353
TAACGCC6450.014.9147294
GTTATCT4350.014.8850583
CGCATCG6100.014.86065613
TAAGACT3250.014.7999994
AATAACG6400.014.4531252
CGCCAGT6300.014.38888918
CGAACGT1803.3360266E-914.3888874
TTATCTA4550.014.230774
ACCGTCG4450.014.1348328
TCGTTTA5650.014.07964630
Produced by FastQC (version 0.11.5)