##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727118.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2808321
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.28042734431	31.0	30.0	31.0	27.0	34.0
2	30.691167783170087	31.0	30.0	33.0	27.0	34.0
3	30.998078923313965	31.0	31.0	34.0	28.0	34.0
4	34.9211037484675	35.0	35.0	37.0	32.0	37.0
5	34.62265424785842	35.0	35.0	37.0	32.0	37.0
6	34.544361203722794	35.0	35.0	37.0	30.0	37.0
7	34.375369838419466	35.0	35.0	37.0	30.0	37.0
8	34.392089793153986	35.0	35.0	37.0	30.0	37.0
9	35.693057168322284	37.0	35.0	39.0	30.0	39.0
10	35.52796884686615	37.0	35.0	39.0	30.0	39.0
11	35.543638351883565	37.0	35.0	39.0	30.0	39.0
12	35.35567087950416	37.0	34.0	39.0	30.0	39.0
13	35.50215733885122	37.0	35.0	39.0	30.0	39.0
14	36.15988022736717	38.0	34.0	40.0	30.0	41.0
15	36.212221822220464	38.0	34.0	40.0	30.0	41.0
16	36.02968855768269	38.0	34.0	40.0	29.0	41.0
17	36.07555688968604	38.0	34.0	40.0	29.0	41.0
18	35.968733631233754	38.0	34.0	40.0	29.0	41.0
19	35.94714671150484	38.0	34.0	40.0	27.0	41.0
20	36.391663915912744	38.0	35.0	40.0	30.0	41.0
21	36.48394610160306	38.0	35.0	40.0	30.0	41.0
22	36.47466439911962	38.0	35.0	40.0	30.0	41.0
23	36.37209314747139	38.0	35.0	40.0	30.0	41.0
24	36.23687320644613	38.0	35.0	40.0	29.0	41.0
25	35.9317140027796	38.0	34.0	40.0	27.0	41.0
26	35.71828576576538	38.0	34.0	40.0	27.0	41.0
27	35.53794669484009	38.0	34.0	40.0	27.0	41.0
28	35.28310972997745	38.0	34.0	40.0	27.0	41.0
29	35.115091544022214	38.0	33.0	40.0	26.0	41.0
30	34.778437721328864	37.0	33.0	40.0	25.0	41.0
31	34.5930411801215	37.0	33.0	40.0	25.0	41.0
32	34.37977068860718	37.0	33.0	40.0	24.0	41.0
33	34.303213913224305	37.0	33.0	40.0	24.0	41.0
34	34.10727691029622	37.0	33.0	40.0	24.0	41.0
35	33.70060438247622	37.0	32.0	40.0	22.0	41.0
36	33.68040939764364	37.0	32.0	40.0	22.0	41.0
37	33.499170144723486	36.0	31.0	40.0	21.0	41.0
38	33.368938237473564	36.0	31.0	40.0	21.0	41.0
39	33.209735639195095	36.0	31.0	39.0	19.0	41.0
40	32.94536735650946	36.0	31.0	39.0	18.0	40.0
41	32.676083325232405	36.0	30.0	39.0	16.0	40.0
42	32.42636044811117	36.0	30.0	39.0	15.0	40.0
43	32.198990784885346	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	9.0
11	18.0
12	18.0
13	28.0
14	55.0
15	134.0
16	257.0
17	495.0
18	992.0
19	2091.0
20	3782.0
21	6721.0
22	11007.0
23	17230.0
24	25071.0
25	34525.0
26	46740.0
27	61449.0
28	77851.0
29	96798.0
30	117873.0
31	142551.0
32	169486.0
33	201995.0
34	235193.0
35	270461.0
36	317426.0
37	361392.0
38	385754.0
39	220916.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.85181323644982	24.345649945287594	16.02615940271785	23.776377415544733
2	18.455618143367513	27.17381666839368	30.59141031242511	23.7791548758137
3	20.571188265159147	26.852663922678353	30.983459511929013	21.592688300233483
4	16.470909130402113	18.33476301320255	31.24503929572154	33.94928856067379
5	13.024009719686603	41.82844482521763	31.586310824154364	13.561234630941406
6	33.029877994716415	37.62960145937733	14.459956678741499	14.880563867164758
7	26.577730964515812	33.855638297758695	20.54665403278329	19.019976704942206
8	29.168816527740237	35.5032775811597	19.10999490442866	16.217910986671395
9	28.471816434089973	14.12491663168135	19.22988860603898	38.17337832818969
10	17.852588788817233	30.721879728136493	30.909678772476507	20.515852710569767
11	34.96174404564151	24.097209685075175	19.237295166756223	21.703751102527097
12	20.686808950971063	30.654330470056664	28.38813654137116	20.270724037601116
13	30.613950470761715	20.675663501430215	25.972600710531314	22.737785317276764
14	22.50326796687416	22.81822483968179	26.590407578051085	28.08809961539297
15	26.050832508107156	29.117967639739188	22.352038816075513	22.47916103607814
16	22.36261453017657	27.209852434960247	26.558644827282922	23.86888820758026
17	22.680242037858207	29.527536204016563	26.109657692265237	21.682564065859992
18	22.894498171683363	25.565880823452876	28.5536090781645	22.986011926699263
19	23.850834715831986	27.59061375106336	27.79482829776226	20.7637232353424
20	23.198558854205057	26.06518271949681	28.604066273050694	22.132192153247438
21	23.42769932639467	27.642886977663878	27.776383112899133	21.153030583042323
22	23.301396101086734	27.570316926020922	26.40652546485961	22.72176150803274
23	21.928974643568168	27.347657194458897	27.69986052164265	23.02350764033029
24	21.829555809325214	29.253529065943674	27.140273494376178	21.776641630354934
25	22.706414259623457	27.927149353652947	26.368566841183753	22.997869545539846
26	22.551980346976006	28.079197499146286	27.45544401797373	21.91337813590398
27	23.017810285932413	28.268955009060576	26.43689948549329	22.276335219513722
28	21.816594328070046	28.55563876066874	28.03906675910624	21.58870015215497
29	22.283599346371016	28.12196326559535	27.92508406268372	21.669353325349917
30	21.89464808332096	29.371321868119775	27.837202371096463	20.8968276774628
31	22.69765457723672	28.688351509674288	26.58738085852721	22.026613054561782
32	20.576137841792303	29.678444878630327	27.215122487778288	22.530294791799086
33	21.27687682426617	28.753550609064987	28.009939034747095	21.959633531921742
34	21.13184354637522	28.976780076066806	27.828941207219543	22.062435170338436
35	21.521863063374877	29.607227948656867	27.881285650750037	20.989623337218216
36	21.74576909121144	29.343760916220045	26.971275719549155	21.939194273019357
37	21.07223497598743	29.44392752822772	27.590471317203413	21.893366178581434
38	21.657175230324455	28.58458844270295	27.94292390364207	21.815312423330525
39	21.52424883052899	27.81334469955536	27.992633320763545	22.66977314915211
40	21.4796670323656	28.62895659007642	28.331198605857377	21.5601777717006
41	20.522547101987275	28.838191930338446	28.436279186033218	22.20298178164106
42	21.37066952104122	29.24024710850362	28.286118289184177	21.10296508127098
43	20.967261221206552	28.025286283156376	28.518783999407475	22.488668496229597
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	799.0
1	1153.5
2	1508.0
3	1534.0
4	1560.0
5	1560.0
6	1998.5
7	2437.0
8	3919.0
9	5401.0
10	9608.0
11	13815.0
12	13815.0
13	30846.5
14	47878.0
15	63891.5
16	79905.0
17	60249.0
18	40593.0
19	40593.0
20	45719.5
21	50846.0
22	44772.5
23	38699.0
24	42067.0
25	45435.0
26	45435.0
27	50441.5
28	55448.0
29	58839.5
30	62231.0
31	67141.0
32	72051.0
33	72051.0
34	81554.5
35	91058.0
36	102078.5
37	113099.0
38	125281.5
39	137464.0
40	137464.0
41	146112.0
42	154760.0
43	164605.0
44	174450.0
45	193298.5
46	212147.0
47	212147.0
48	252383.0
49	292619.0
50	279896.0
51	267173.0
52	226847.5
53	186522.0
54	186522.0
55	170973.0
56	155424.0
57	146461.0
58	137498.0
59	126034.5
60	114571.0
61	114571.0
62	97395.0
63	80219.0
64	69047.0
65	57875.0
66	48988.5
67	40102.0
68	40102.0
69	32725.0
70	25348.0
71	22580.5
72	19813.0
73	15706.0
74	11599.0
75	11599.0
76	8974.5
77	6350.0
78	5396.5
79	4443.0
80	3502.5
81	2562.0
82	2562.0
83	2094.0
84	1626.0
85	1338.5
86	1051.0
87	1037.0
88	1023.0
89	1023.0
90	723.5
91	424.0
92	331.5
93	239.0
94	218.5
95	198.0
96	198.0
97	112.0
98	26.0
99	29.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2808321.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.66570297586358
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.90168402744688	46.099742601434485
2	7.40095404662419	7.943548032082988
3	2.409424940968253	3.8791044967391977
4	1.1788457237464953	2.5305433785978537
5	0.6985041690935412	1.8742858632988184
6	0.4600814960964379	1.4814358148521425
7	0.3055620349668172	1.1478740986460758
8	0.2354002281939095	1.0106334981363883
9	0.18617411438739964	0.8992048252057775
>10	1.05451022125442	10.376950441065866
>50	0.08658288944762474	3.2082272062613493
>100	0.06816802666961833	7.391672447651598
>500	0.008318444047511272	3.0042668028709314
>1k	0.005124161533259686	4.751678542929824
>5k	4.658328666599715E-4	1.6538788383633354
>10k+	1.9964265713998778E-4	2.7469531118633697
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39163	1.3945343142753268	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22244	0.7920746951648334	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15510	0.5522872919441901	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8117	0.2890339102972915	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	8019	0.28554428072859195	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	7359	0.2620426938373498	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	6113	0.2176745464638836	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5863	0.20877243021720096	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	5690	0.2026121657744966	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5149	0.18334798621667536	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	4984	0.17747258949386482	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	4899	0.17444586996999276	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	4648	0.1655081452583234	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3931	0.1399768758628376	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	3906	0.13908666423816934	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	2934	0.1044752362710673	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2814	0.10020222047265963	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.5608464986730504E-5
2	0.0	0.0	0.0	1.0682539496019151E-4	3.5608464986730504E-5
3	0.0	0.0	0.0	1.4243385994692202E-4	3.5608464986730504E-5
4	0.0	0.0	0.0	1.7804232493365252E-4	3.5608464986730504E-5
5	0.0	7.121692997346101E-5	0.0	2.1365078992038303E-4	3.5608464986730504E-5
6	0.0	7.121692997346101E-5	0.0	2.4925925490711353E-4	3.5608464986730504E-5
7	0.0	7.121692997346101E-5	0.0	2.4925925490711353E-4	3.5608464986730504E-5
8	0.0	7.121692997346101E-5	0.0	2.4925925490711353E-4	3.5608464986730504E-5
9	0.0	7.121692997346101E-5	0.0	2.4925925490711353E-4	3.5608464986730504E-5
10	0.0	7.121692997346101E-5	0.0	2.4925925490711353E-4	3.5608464986730504E-5
11	0.0	7.121692997346101E-5	0.0	2.4925925490711353E-4	7.121692997346101E-5
12	0.0	7.121692997346101E-5	0.0	3.2047618488057454E-4	7.121692997346101E-5
13	0.0	7.121692997346101E-5	0.0	3.2047618488057454E-4	1.0682539496019151E-4
14	0.0	7.121692997346101E-5	0.0	4.985185098142271E-4	1.4243385994692202E-4
15	0.0	7.121692997346101E-5	0.0	9.614285546417237E-4	1.4243385994692202E-4
16	0.0	7.121692997346101E-5	0.0	0.0021365078992038305	2.4925925490711353E-4
17	0.0	7.121692997346101E-5	0.0	0.003169153383819015	2.8486771989384404E-4
18	0.0	7.121692997346101E-5	0.0	0.0039525396135270865	3.2047618488057454E-4
19	0.0	7.121692997346101E-5	0.0	0.00562613746790342	3.2047618488057454E-4
20	0.0	7.121692997346101E-5	0.0	0.007406560717239945	3.2047618488057454E-4
21	0.0	7.121692997346101E-5	0.0	0.01271222200026279	3.2047618488057454E-4
22	0.0	7.121692997346101E-5	0.0	0.020332433507423118	3.2047618488057454E-4
23	0.0	7.121692997346101E-5	0.0	0.03567968191670397	4.2730157984076605E-4
24	0.0	7.121692997346101E-5	0.0	0.06313380842147319	4.2730157984076605E-4
25	0.0	7.121692997346101E-5	0.0	0.07930005152544883	4.2730157984076605E-4
26	0.0	7.121692997346101E-5	0.0	0.10059391358751368	4.6291004482749656E-4
27	0.0	7.121692997346101E-5	0.0	0.13114597654612845	4.6291004482749656E-4
28	0.0	7.121692997346101E-5	0.0	0.18537766872091901	4.6291004482749656E-4
29	0.0	7.121692997346101E-5	0.0	0.24320581585936935	4.6291004482749656E-4
30	0.0	7.121692997346101E-5	0.0	0.33795994118905925	4.6291004482749656E-4
31	0.0	7.121692997346101E-5	0.0	0.5493317893502915	4.6291004482749656E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTCGG	515	0.0	16.883495	37
AAGACGG	680	0.0	16.323528	5
CAAGACG	715	0.0	16.041958	4
CGCTTCG	410	0.0	15.792684	32
GGTTATC	375	0.0	15.786666	2
ATGGTCG	540	0.0	15.759259	36
TAGAGTG	425	0.0	15.6705885	5
TTAGAGT	445	0.0	15.382023	4
GTAAACG	450	0.0	15.211111	27
ATAACGC	680	0.0	14.963235	3
TAACGCC	645	0.0	14.914729	4
GTTATCT	435	0.0	14.885058	3
CGCATCG	610	0.0	14.860656	13
TAAGACT	325	0.0	14.799999	4
AATAACG	640	0.0	14.453125	2
CGCCAGT	630	0.0	14.388889	18
CGAACGT	180	3.3360266E-9	14.388887	4
TTATCTA	455	0.0	14.23077	4
ACCGTCG	445	0.0	14.134832	8
TCGTTTA	565	0.0	14.079646	30
>>END_MODULE