FastQCFastQC Report
Thu 9 Feb 2017
SRR2727117.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727117.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2582289
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT390981.5140830480244465No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203840.7893771766057168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT152460.5904064184915012No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT84210.3261060245386942No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58980.22840201077416197No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49450.19149676895188728No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC47000.1820090625022993No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG46950.18181543584006282No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA36060.1396435488049556No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG33870.1311627009989974No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33240.12872300505481765No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA28540.11052209880458772No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG28130.1089343601742485No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT28120.10889563484180122No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT26820.10386134162365251No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG26410.1022736029933133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAT1205.182301E-918.51
CGAACGT2550.018.1372554
CAAGACG9000.017.6777784
AAGACGG8300.017.6084355
TCGAACG2700.017.129633
TTAGTAC904.449411E-516.4444453
ATACCGT5150.016.1650496
CGCAAGA9300.015.9139792
GCGCAAG9150.015.7704911
TACCGTC4600.015.6847837
TTTTACG957.065125E-515.5789482
TATCTAG6900.015.2826091
TAGAGTC7800.014.9423085
TAGACGG750.00410624814.85
TTAGAGT3500.014.84
ATAACAC2006.184564E-1114.83
AGGTTAT4150.014.7108431
CCGTCGT5050.014.6534649
CGTCGTA5050.014.65346410
ACCGTCG5050.014.6534648
Produced by FastQC (version 0.11.5)