##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727117.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2582289
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.25355837398525	31.0	30.0	31.0	27.0	34.0
2	30.67821610981575	31.0	30.0	33.0	27.0	34.0
3	30.98474260626909	31.0	31.0	34.0	28.0	34.0
4	34.920856650824135	35.0	35.0	37.0	32.0	37.0
5	34.620465796043746	35.0	35.0	37.0	32.0	37.0
6	34.531635692209505	35.0	35.0	37.0	30.0	37.0
7	34.37854322269893	35.0	35.0	37.0	30.0	37.0
8	34.39967757288204	35.0	35.0	37.0	30.0	37.0
9	35.679735304607654	37.0	35.0	39.0	30.0	39.0
10	35.52010483722	37.0	35.0	39.0	30.0	39.0
11	35.52344373538361	37.0	35.0	39.0	30.0	39.0
12	35.32779212551345	37.0	34.0	39.0	30.0	39.0
13	35.48101161411446	37.0	34.0	39.0	30.0	39.0
14	36.12313106704943	38.0	34.0	40.0	29.0	41.0
15	36.181355766143916	38.0	34.0	40.0	30.0	41.0
16	36.003030644517324	38.0	34.0	40.0	29.0	41.0
17	36.038537901838254	38.0	34.0	40.0	29.0	41.0
18	35.94325189783173	38.0	34.0	40.0	29.0	41.0
19	35.9146942886718	38.0	34.0	40.0	27.0	41.0
20	36.35823914364349	38.0	34.0	40.0	30.0	41.0
21	36.43950890082404	38.0	35.0	40.0	30.0	41.0
22	36.41019111338816	38.0	35.0	40.0	30.0	41.0
23	36.31623493729788	38.0	35.0	40.0	30.0	41.0
24	36.164110601098486	38.0	34.0	40.0	29.0	41.0
25	35.84598238229726	38.0	34.0	40.0	27.0	41.0
26	35.60905111705158	38.0	34.0	40.0	27.0	41.0
27	35.41916299840955	38.0	34.0	40.0	27.0	41.0
28	35.15071357233834	38.0	33.0	40.0	26.0	41.0
29	34.95588216500941	38.0	33.0	40.0	25.0	41.0
30	34.6185109412618	37.0	33.0	40.0	25.0	41.0
31	34.4271926960925	37.0	33.0	40.0	24.0	41.0
32	34.219215587411014	37.0	33.0	40.0	24.0	41.0
33	34.12718560935666	37.0	33.0	40.0	24.0	41.0
34	33.92675955324908	37.0	32.0	40.0	23.0	41.0
35	33.48994090127015	36.0	31.0	40.0	21.0	41.0
36	33.447391442243685	36.0	31.0	40.0	21.0	41.0
37	33.27659103996493	36.0	31.0	39.0	20.0	41.0
38	33.136866167961834	36.0	31.0	39.0	19.0	41.0
39	32.98210928366267	36.0	31.0	39.0	18.0	40.0
40	32.68633913554989	36.0	30.0	39.0	16.0	40.0
41	32.40320545066799	35.0	30.0	39.0	15.0	40.0
42	32.138812503170634	35.0	30.0	39.0	15.0	40.0
43	31.90357159868628	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	8.0
11	15.0
12	14.0
13	30.0
14	78.0
15	159.0
16	252.0
17	504.0
18	1071.0
19	2104.0
20	4111.0
21	6857.0
22	11216.0
23	17423.0
24	24825.0
25	34040.0
26	45148.0
27	58769.0
28	73974.0
29	91384.0
30	110385.0
31	132485.0
32	158249.0
33	185636.0
34	216690.0
35	248336.0
36	289356.0
37	326031.0
38	344004.0
39	199129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.43681594120565	24.701611632160457	16.613128894558276	23.248443532075612
2	18.957366894255443	26.88440372088484	30.911993196733594	23.246236188126115
3	20.411193324991896	26.62052930558896	31.273881428453592	21.694395940965556
4	16.132508793554866	18.2278203562808	31.499533940623998	34.14013690954034
5	12.71186145315261	42.09513342619668	31.521839732113637	13.671165388537068
6	32.67116887381699	38.54289740613851	14.185902507426551	14.600031212617953
7	26.732058263037175	34.0411162344726	20.20362554307438	19.02319995941585
8	29.204089859810423	35.61874755304306	18.7180056143987	16.45915697274782
9	28.4993662599345	14.054120201108397	18.980485917726483	38.46602762123062
10	17.706732282869968	31.040600025791072	30.451123015278302	20.801544676060658
11	35.414587600380905	24.01958107709865	19.069476731690372	21.496354590830073
12	20.637581618478798	30.929497046999778	27.95248711511376	20.480434219407666
13	30.428894674453556	20.826406339491825	25.84946921123081	22.895229774823807
14	22.39439505028291	22.953588850821887	26.521818433180794	28.130197665714412
15	25.768804343743092	29.83484807471201	21.958463982923675	22.437883598621223
16	22.455890878209217	27.63505556504326	25.828402630379482	24.08065092636804
17	22.463907022025808	29.72142157597387	25.69902129467306	22.11565010732726
18	22.70272614722829	26.022648897935124	28.28490536884137	22.989719585995218
19	23.90441193840039	27.190256396553604	27.885259937985253	21.02007172706076
20	23.300645280214567	26.175846313096635	28.20915861857445	22.314349788114342
21	23.908865351631828	27.478411595293945	27.608451261651968	21.004271791422262
22	23.467280385735293	27.44421712674298	26.440805037701047	22.647697449820683
23	22.03320387454696	27.41195892481438	27.756382031600644	22.79845516903801
24	22.089355606595543	28.639668139391063	27.266893829466802	22.004082424546596
25	22.56517376637549	27.813927875617328	26.73322002301059	22.887678334996586
26	22.677825758464678	28.15842843306849	27.311582863110985	21.852162945355847
27	22.970550546433806	28.319758168043936	26.43267271788711	22.27701856763515
28	21.868621211645948	28.665459210800957	27.904854956203586	21.56106462134951
29	22.49957305320977	28.17635826199159	27.766334442039604	21.55773424275904
30	22.087264438643388	29.321737419785315	27.51225753585288	21.078740605718416
31	22.60347312016587	28.749880435536067	26.737479809579796	21.909166634718265
32	20.95195386728596	29.647185113672403	26.953025009981452	22.44783600906018
33	21.250255103127497	28.824620327159355	27.72594392029707	22.199180649416082
34	21.347571863567556	28.98920299006037	27.660575559126034	22.002649587246044
35	21.744119267827884	29.381451882419046	27.64210357554867	21.232325274204396
36	21.535428451269397	29.38311707171428	27.00356156882518	22.077892908191142
37	21.17435345153079	29.265392061074497	27.531232948752056	22.029021538642652
38	21.568693511841623	28.39573727030553	28.052669550154917	21.98289966769792
39	21.52745103278525	27.929097014315595	27.955972395034017	22.587479557865134
40	21.491359022944373	28.446970885133304	28.416765125824412	21.644904966097908
41	20.526710991682187	28.77431612031031	28.585646300627076	22.11332658738042
42	21.213311135972774	28.960817321376496	28.47156147123734	21.354310071413384
43	20.756778191751582	28.03632745986216	28.589867361863835	22.617026986522422
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1145.0
1	1387.5
2	1630.0
3	1665.5
4	1701.0
5	1701.0
6	2286.0
7	2871.0
8	4466.0
9	6061.0
10	10543.5
11	15026.0
12	15026.0
13	31081.0
14	47136.0
15	63065.0
16	78994.0
17	58655.5
18	38317.0
19	38317.0
20	42483.5
21	46650.0
22	40065.5
23	33481.0
24	36571.0
25	39661.0
26	39661.0
27	43796.5
28	47932.0
29	51391.0
30	54850.0
31	59361.0
32	63872.0
33	63872.0
34	73063.0
35	82254.0
36	93222.0
37	104190.0
38	116207.5
39	128225.0
40	128225.0
41	136993.0
42	145761.0
43	154124.0
44	162487.0
45	178365.0
46	194243.0
47	194243.0
48	218271.5
49	242300.0
50	234646.5
51	226993.0
52	201990.5
53	176988.0
54	176988.0
55	163209.5
56	149431.0
57	141678.5
58	133926.0
59	122556.5
60	111187.0
61	111187.0
62	94062.5
63	76938.0
64	66443.5
65	55949.0
66	47276.5
67	38604.0
68	38604.0
69	31572.0
70	24540.0
71	21914.5
72	19289.0
73	15328.5
74	11368.0
75	11368.0
76	8809.0
77	6250.0
78	5374.5
79	4499.0
80	3593.0
81	2687.0
82	2687.0
83	2223.5
84	1760.0
85	1403.5
86	1047.0
87	1059.0
88	1071.0
89	1071.0
90	762.5
91	454.0
92	357.0
93	260.0
94	222.5
95	185.0
96	185.0
97	115.0
98	45.0
99	38.0
100	31.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2582289.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.22154185585527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.36987907612425	47.694778924873546
2	7.105246547300621	7.847253392158649
3	2.3671822668022307	3.921583638799732
4	1.1354121965406683	2.5079684853967636
5	0.6978632304671625	1.9268541795452394
6	0.4448000045955057	1.47375252427532
7	0.32022621804420187	1.2378369852149091
8	0.23394500847996746	1.0335043262195953
9	0.18340684615544015	0.9115207948460743
>10	0.9844709859304899	9.961797669910775
>50	0.07948934860726052	3.060396160189059
>100	0.06469839610273104	7.493692117224748
>500	0.008872969637012307	3.395166042605049
>1k	0.004154803242727411	4.071103827831191
>5k	1.408407878890648E-4	0.5568262079541009
>10k+	2.1126118183359717E-4	2.905964722955098
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39098	1.5140830480244465	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20384	0.7893771766057168	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15246	0.5904064184915012	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8421	0.3261060245386942	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5898	0.22840201077416197	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4945	0.19149676895188728	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	4700	0.1820090625022993	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	4695	0.18181543584006282	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	3606	0.1396435488049556	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	3387	0.1311627009989974	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3324	0.12872300505481765	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	2854	0.11052209880458772	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	2813	0.1089343601742485	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2812	0.10889563484180122	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	2682	0.10386134162365251	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	2641	0.1022736029933133	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.8725332447297724E-5
2	0.0	0.0	0.0	0.0	3.8725332447297724E-5
3	0.0	0.0	0.0	0.0	3.8725332447297724E-5
4	0.0	0.0	0.0	0.0	3.8725332447297724E-5
5	0.0	0.0	0.0	0.0	3.8725332447297724E-5
6	0.0	0.0	0.0	0.0	3.8725332447297724E-5
7	0.0	0.0	0.0	3.8725332447297724E-5	3.8725332447297724E-5
8	0.0	0.0	0.0	3.8725332447297724E-5	3.8725332447297724E-5
9	0.0	0.0	0.0	1.1617599734189319E-4	1.1617599734189319E-4
10	0.0	0.0	0.0	1.1617599734189319E-4	1.1617599734189319E-4
11	0.0	0.0	0.0	1.549013297891909E-4	1.1617599734189319E-4
12	0.0	0.0	0.0	1.9362666223648865E-4	1.1617599734189319E-4
13	0.0	0.0	0.0	2.3235199468378637E-4	1.1617599734189319E-4
14	0.0	0.0	0.0	4.25978656920275E-4	1.549013297891909E-4
15	0.0	0.0	0.0	0.0010843093085243364	1.549013297891909E-4
16	0.0	0.0	0.0	0.00255587194152165	1.549013297891909E-4
17	0.0	0.0	0.0	0.005498997207516277	1.549013297891909E-4
18	3.8725332447297724E-5	3.8725332447297724E-5	0.0	0.006157327859120339	1.549013297891909E-4
19	3.8725332447297724E-5	3.8725332447297724E-5	0.0	0.007783791821906843	1.549013297891909E-4
20	3.8725332447297724E-5	3.8725332447297724E-5	0.0	0.010029861103850112	1.549013297891909E-4
21	3.8725332447297724E-5	3.8725332447297724E-5	0.0	0.01568375964115558	1.549013297891909E-4
22	3.8725332447297724E-5	3.8725332447297724E-5	0.0	0.025094015425848926	1.549013297891909E-4
23	3.8725332447297724E-5	3.8725332447297724E-5	0.0	0.03977091642337476	1.9362666223648865E-4
24	3.8725332447297724E-5	3.8725332447297724E-5	0.0	0.06486493184922369	1.9362666223648865E-4
25	3.8725332447297724E-5	3.8725332447297724E-5	0.0	0.0750109689504157	1.9362666223648865E-4
26	3.8725332447297724E-5	3.8725332447297724E-5	0.0	0.09050110192933479	2.3235199468378637E-4
27	3.8725332447297724E-5	3.8725332447297724E-5	0.0	0.11129660545353366	2.710773271310841E-4
28	3.8725332447297724E-5	3.8725332447297724E-5	0.0	0.15373956981577197	3.098026595783818E-4
29	3.8725332447297724E-5	3.8725332447297724E-5	0.0	0.21178884315427127	3.4852799202567954E-4
30	3.8725332447297724E-5	3.8725332447297724E-5	0.0	0.3118163768656413	3.4852799202567954E-4
31	3.8725332447297724E-5	3.8725332447297724E-5	0.0	0.5481570807914993	3.4852799202567954E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAAT	120	5.182301E-9	18.5	1
CGAACGT	255	0.0	18.137255	4
CAAGACG	900	0.0	17.677778	4
AAGACGG	830	0.0	17.608435	5
TCGAACG	270	0.0	17.12963	3
TTAGTAC	90	4.449411E-5	16.444445	3
ATACCGT	515	0.0	16.165049	6
CGCAAGA	930	0.0	15.913979	2
GCGCAAG	915	0.0	15.770491	1
TACCGTC	460	0.0	15.684783	7
TTTTACG	95	7.065125E-5	15.578948	2
TATCTAG	690	0.0	15.282609	1
TAGAGTC	780	0.0	14.942308	5
TAGACGG	75	0.004106248	14.8	5
TTAGAGT	350	0.0	14.8	4
ATAACAC	200	6.184564E-11	14.8	3
AGGTTAT	415	0.0	14.710843	1
CCGTCGT	505	0.0	14.653464	9
CGTCGTA	505	0.0	14.653464	10
ACCGTCG	505	0.0	14.653464	8
>>END_MODULE