FastQCFastQC Report
Thu 9 Feb 2017
SRR2727116.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727116.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3361137
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT544581.6202255367752043No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297190.884194842400057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT211860.6303224176818737No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT122590.36472776920429006No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG91550.27237806730282044No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC84600.25170054062062924No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79270.23584281152479056No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT67750.20156869535517297No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG59330.17651764864092123No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA58350.1736019686195475No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52110.1550368223610046No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT49960.14864017741615412No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG48370.14390963534066No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG45290.13474606955919977No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA44480.13233617076602353No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA41700.12406516009314705No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT37620.11192641061640747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG9200.017.2934764
TTTGCGT5100.016.6862743
AAGACGG9450.016.6402115
TTTTGCG4800.016.5729182
GGTTATC5350.016.2523372
TATCTAG9050.016.1491721
TTGCGTT5300.016.0566044
AGGTTAT5800.015.9482761
ACGAACG3850.015.85714215
TCGTTTA6200.015.516129530
CGTCGTA7300.015.2054810
ACACGCT7950.014.8930819
TGCGTAC3000.014.79999916
CGAACGA4150.014.71084316
CGCAAGA11350.014.6696032
TACGACG7200.014.6458335
TACCGTC7200.014.6458337
ATACCGT7750.014.5612916
GTGCGTA3050.014.55737715
TTATCTA6750.014.5259274
Produced by FastQC (version 0.11.5)