##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727116.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3361137
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.44793681423875	31.0	30.0	31.0	28.0	34.0
2	30.83747910305352	31.0	31.0	34.0	28.0	34.0
3	31.14174340409213	31.0	31.0	34.0	28.0	34.0
4	35.062197107704925	35.0	35.0	37.0	32.0	37.0
5	34.77601210542742	35.0	35.0	37.0	32.0	37.0
6	34.68375046896333	35.0	35.0	37.0	32.0	37.0
7	34.4953779033702	35.0	35.0	37.0	30.0	37.0
8	34.53811254941408	35.0	35.0	37.0	30.0	37.0
9	35.83718128716562	37.0	35.0	39.0	30.0	39.0
10	35.67498706538888	37.0	35.0	39.0	30.0	39.0
11	35.673612828040035	37.0	35.0	39.0	30.0	39.0
12	35.50491187952172	37.0	35.0	39.0	30.0	39.0
13	35.63552839411187	37.0	35.0	39.0	30.0	39.0
14	36.31392115227674	38.0	35.0	40.0	30.0	41.0
15	36.362350002395026	38.0	35.0	40.0	30.0	41.0
16	36.179012340169415	38.0	34.0	40.0	29.0	41.0
17	36.233779819150485	38.0	34.0	40.0	30.0	41.0
18	36.14374153746188	38.0	34.0	40.0	29.0	41.0
19	36.09272011227153	38.0	34.0	40.0	29.0	41.0
20	36.57557516994993	38.0	35.0	40.0	30.0	41.0
21	36.664752433477126	38.0	35.0	40.0	30.0	41.0
22	36.65541184426579	39.0	35.0	40.0	30.0	41.0
23	36.56715569761066	38.0	35.0	40.0	30.0	41.0
24	36.404475330818116	38.0	35.0	40.0	30.0	41.0
25	36.09328033936135	38.0	34.0	40.0	29.0	41.0
26	35.85905275506473	38.0	34.0	40.0	27.0	41.0
27	35.670714404084094	38.0	34.0	40.0	27.0	41.0
28	35.407095277580176	38.0	34.0	40.0	27.0	41.0
29	35.21033150389288	38.0	34.0	40.0	26.0	41.0
30	34.8630043940488	38.0	33.0	40.0	25.0	41.0
31	34.663155354869495	37.0	33.0	40.0	25.0	41.0
32	34.43606731888644	37.0	33.0	40.0	24.0	41.0
33	34.34263167493619	37.0	33.0	40.0	24.0	41.0
34	34.13531908993891	37.0	33.0	40.0	24.0	41.0
35	33.70552911113114	37.0	32.0	40.0	22.0	41.0
36	33.67008277258559	37.0	32.0	40.0	22.0	41.0
37	33.48493024830586	36.0	32.0	40.0	21.0	41.0
38	33.31774932113746	36.0	31.0	40.0	20.0	41.0
39	33.13795480517456	36.0	31.0	40.0	19.0	41.0
40	32.83427215254838	36.0	31.0	39.0	17.0	41.0
41	32.54483557201031	36.0	30.0	39.0	15.0	41.0
42	32.268361271795825	35.0	30.0	39.0	15.0	40.0
43	32.01437638513396	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	12.0
11	24.0
12	30.0
13	45.0
14	90.0
15	178.0
16	352.0
17	615.0
18	1208.0
19	2397.0
20	4468.0
21	7880.0
22	12995.0
23	20269.0
24	29244.0
25	40958.0
26	54465.0
27	71222.0
28	90277.0
29	112244.0
30	136569.0
31	165282.0
32	199125.0
33	236903.0
34	277989.0
35	321312.0
36	379184.0
37	435322.0
38	459780.0
39	300694.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.05243195978027	23.473039034112563	15.789805652075472	23.684723354031686
2	18.324453897594772	26.777545812622332	30.938012940263963	23.959987349518926
3	20.21827732698786	26.886645798728225	30.97109698295547	21.92397989132844
4	15.923986436732571	17.794246411259046	31.034051869947582	35.2477152820608
5	12.878201632364286	42.05770249769646	31.21229512513176	13.851800744807486
6	33.040366994859184	37.463780857489596	14.612674223038216	14.883177924613012
7	26.994853229725535	33.86440362294069	20.15817861634322	18.982564530990555
8	29.949389150159604	34.76293885075199	18.78926684630826	16.498405152780148
9	28.888944425651204	13.949386770012648	19.12427253039671	38.03739627393944
10	18.17316580668982	30.736473996745744	30.031831490355792	21.058528706208644
11	34.94144987246875	24.238464543397072	19.03385669789717	21.78622888623701
12	21.16926504334694	30.87107130712018	27.751561450782873	20.208102198750005
13	30.559688581572246	20.432074027330632	25.9479158391937	23.060321551903417
14	22.681312900961785	22.983889082771693	26.39377686776826	27.941021148498262
15	26.20047918308596	29.229245936717245	22.125756849542284	22.44451803065451
16	22.42907682727601	27.09160620349602	26.325139379918166	24.154177589309807
17	22.68631120956986	29.493680263553674	25.866574316964762	21.953434209911705
18	23.010516976844443	25.39539447514338	28.43272975781707	23.161358790195102
19	23.824140461992474	27.13441909687109	28.00382727630561	21.03761316483083
20	23.342041696009417	26.00340301511066	28.41556294789531	22.238992340984613
21	23.901822508276215	27.445712566908163	27.702322160626004	20.950142764189618
22	23.463607701798527	27.337415880399995	26.32026007865791	22.87871633914357
23	21.879441391410108	27.396860050631677	27.64266972753565	23.08102883042256
24	21.95361272093342	29.089263543854354	27.09175496268078	21.865368772531436
25	22.600536663634955	27.901480957188003	26.38993888080135	23.1080434983757
26	22.7022284423396	28.136907242995452	27.366453673265923	21.794410641399026
27	22.930514287278385	28.263858331273017	26.420256002656245	22.385371378792353
28	21.775042195542756	28.713854865184015	28.00879583307672	21.502307106196504
29	22.297990233662002	28.209769491692843	27.90719330988293	21.58504696476222
30	21.7893230772801	29.670316919542405	27.737310320882486	20.803049682295008
31	22.58586900801723	28.853212469470897	26.48579929946325	22.075119223048628
32	20.488513262030082	29.832523934609036	27.09217148839812	22.586791314962763
33	21.04049909301525	28.967578530717432	27.913232932784354	22.078689443482965
34	21.10631015635483	29.10684687949346	27.74022600090386	22.046616963247853
35	21.506918640924187	29.642945229545838	27.776791008518842	21.073345121011133
36	21.43396713671594	29.55949132689325	27.071315450694218	21.935226085696595
37	21.03240659336409	29.48897947331513	27.536366414103323	21.942247519217457
38	21.34697276546597	28.832088665234412	28.05714256812501	21.7637960011746
39	21.461844607940705	27.949083896312466	27.895381830612674	22.69368966513415
40	21.228679461741667	28.87198587858811	28.389738353420285	21.50959630624994
41	20.234849100170567	29.12264510491539	28.56119818977923	22.08130760513481
42	21.179083149541363	29.310408947924465	28.47694098752892	21.03356691500525
43	20.71090824325221	28.149224503493908	28.573485698440738	22.566381554813148
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1268.0
1	1765.0
2	2262.0
3	2234.5
4	2207.0
5	2207.0
6	2904.5
7	3602.0
8	5576.0
9	7550.0
10	13270.5
11	18991.0
12	18991.0
13	41944.0
14	64897.0
15	86303.0
16	107709.0
17	80293.0
18	52877.0
19	52877.0
20	58898.5
21	64920.0
22	55300.0
23	45680.0
24	49631.5
25	53583.0
26	53583.0
27	58709.0
28	63835.0
29	66742.5
30	69650.0
31	74221.0
32	78792.0
33	78792.0
34	89298.0
35	99804.0
36	113549.0
37	127294.0
38	142839.5
39	158385.0
40	158385.0
41	168559.0
42	178733.0
43	189958.5
44	201184.0
45	224303.0
46	247422.0
47	247422.0
48	288772.5
49	330123.0
50	318795.5
51	307468.0
52	265493.0
53	223518.0
54	223518.0
55	207811.0
56	192104.0
57	183226.0
58	174348.0
59	161288.5
60	148229.0
61	148229.0
62	126432.5
63	104636.0
64	90459.0
65	76282.0
66	64367.0
67	52452.0
68	52452.0
69	43000.0
70	33548.0
71	30192.0
72	26836.0
73	21378.5
74	15921.0
75	15921.0
76	12211.5
77	8502.0
78	7285.0
79	6068.0
80	4771.0
81	3474.0
82	3474.0
83	2932.5
84	2391.0
85	1972.0
86	1553.0
87	1555.0
88	1557.0
89	1557.0
90	1109.0
91	661.0
92	528.5
93	396.0
94	351.0
95	306.0
96	306.0
97	180.5
98	55.0
99	59.5
100	64.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3361137.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.591158401162424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.96522596504865	42.13523979423286
2	7.7486706056378685	7.685311028052374
3	2.5419457501374443	3.78174103026683
4	1.2550737957576281	2.489622536422589
5	0.786566458278187	1.950337091275745
6	0.48895059833841253	1.4548575943526019
7	0.35521362512314353	1.2330818604813059
8	0.26502765494801045	1.0514422733772324
9	0.19663059250752224	0.8776024973599462
>10	1.2050057333913822	11.04503649504477
>50	0.0959648636886065	3.2976203404166653
>100	0.07612563181198442	7.765285137186602
>500	0.011578323888168205	3.9292025789310387
>1k	0.007357059970322789	6.31188049662018
>5k	4.2212639173983213E-4	1.474213553180527
>10k+	2.4121508099418982E-4	3.517525692798603
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	54458	1.6202255367752043	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29719	0.884194842400057	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21186	0.6303224176818737	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12259	0.36472776920429006	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	9155	0.27237806730282044	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	8460	0.25170054062062924	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7927	0.23584281152479056	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6775	0.20156869535517297	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	5933	0.17651764864092123	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	5835	0.1736019686195475	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5211	0.1550368223610046	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	4996	0.14864017741615412	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	4837	0.14390963534066	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	4529	0.13474606955919977	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	4448	0.13233617076602353	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	4170	0.12406516009314705	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3762	0.11192641061640747	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.9751836952793058E-5	0.0	0.0	0.0	0.0
6	2.9751836952793058E-5	0.0	0.0	0.0	0.0
7	2.9751836952793058E-5	0.0	0.0	0.0	2.9751836952793058E-5
8	5.9503673905586116E-5	0.0	0.0	0.0	5.9503673905586116E-5
9	5.9503673905586116E-5	0.0	0.0	8.925551085837917E-5	5.9503673905586116E-5
10	5.9503673905586116E-5	0.0	0.0	1.7851102171675834E-4	5.9503673905586116E-5
11	5.9503673905586116E-5	0.0	0.0	2.0826285866955142E-4	5.9503673905586116E-5
12	5.9503673905586116E-5	0.0	0.0	2.975183695279306E-4	5.9503673905586116E-5
13	5.9503673905586116E-5	0.0	0.0	4.1652571733910283E-4	5.9503673905586116E-5
14	5.9503673905586116E-5	0.0	0.0	8.628032716309987E-4	8.925551085837917E-5
15	5.9503673905586116E-5	0.0	0.0	0.0011008179672533432	8.925551085837917E-5
16	1.1900734781117223E-4	0.0	0.0	0.0022908914453650653	1.487591847639653E-4
17	1.1900734781117223E-4	0.0	0.0	0.004433023705966165	1.487591847639653E-4
18	1.1900734781117223E-4	0.0	0.0	0.005325578814549957	1.7851102171675834E-4
19	1.1900734781117223E-4	0.0	0.0	0.007735477607726195	1.7851102171675834E-4
20	1.1900734781117223E-4	0.0	0.0	0.010383391096524777	1.7851102171675834E-4
21	1.1900734781117223E-4	0.0	0.0	0.01701805073699763	1.7851102171675834E-4
22	1.1900734781117223E-4	0.0	0.0	0.02850225980077575	1.7851102171675834E-4
23	1.1900734781117223E-4	0.0	0.0	0.047454179939704924	2.975183695279306E-4
24	1.1900734781117223E-4	0.0	0.0	0.07982417854434377	2.975183695279306E-4
25	1.1900734781117223E-4	0.0	0.0	0.09654471091181348	2.975183695279306E-4
26	1.1900734781117223E-4	0.0	0.0	0.11859082209383313	2.975183695279306E-4
27	1.1900734781117223E-4	0.0	0.0	0.1512583390679999	2.975183695279306E-4
28	1.1900734781117223E-4	0.0	0.0	0.20897690275641845	2.975183695279306E-4
29	1.1900734781117223E-4	0.0	0.0	0.2751152363024774	2.975183695279306E-4
30	1.1900734781117223E-4	0.0	0.0	0.3858218216038204	2.975183695279306E-4
31	1.1900734781117223E-4	0.0	0.0	0.621813392313375	2.975183695279306E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGACG	920	0.0	17.293476	4
TTTGCGT	510	0.0	16.686274	3
AAGACGG	945	0.0	16.640211	5
TTTTGCG	480	0.0	16.572918	2
GGTTATC	535	0.0	16.252337	2
TATCTAG	905	0.0	16.149172	1
TTGCGTT	530	0.0	16.056604	4
AGGTTAT	580	0.0	15.948276	1
ACGAACG	385	0.0	15.857142	15
TCGTTTA	620	0.0	15.5161295	30
CGTCGTA	730	0.0	15.20548	10
ACACGCT	795	0.0	14.893081	9
TGCGTAC	300	0.0	14.799999	16
CGAACGA	415	0.0	14.710843	16
CGCAAGA	1135	0.0	14.669603	2
TACGACG	720	0.0	14.645833	5
TACCGTC	720	0.0	14.645833	7
ATACCGT	775	0.0	14.561291	6
GTGCGTA	305	0.0	14.557377	15
TTATCTA	675	0.0	14.525927	4
>>END_MODULE