FastQCFastQC Report
Thu 9 Feb 2017
SRR2727115.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727115.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1715837
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT295731.7235320138218257No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184661.0762094534620712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT117480.6846804212754475No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG84300.49130540954647794No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC75940.4425828327515958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65860.3838359937453266No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA60710.3538214877054172No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG53640.3126171075690756No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52420.30550687507030094No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT48850.2847007029222473No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG45580.26564294860176113No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42670.24868329567435601No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG40170.2341131471112932No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35370.20613846187021262No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA30760.1792711079199248No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA26690.15555090605925856No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT26240.15292827931790723No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC24330.14179668581572727No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC23480.1368428353042859No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC23360.1361434681732589No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22190.12932463864574548No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA21750.12676029249864645No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG20790.12116535545043032No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA20680.12052426891365556No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA17830.10391429955176396No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17730.10333149360924143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACT1306.9667294E-1018.54
TTAGAGT2000.017.5754
AGGTTAT2000.017.5751
TAGAGTG2150.017.2093035
TATCTAG3050.016.377051
AAGACGG3350.016.0149255
CGAACGT1059.34839E-615.8571424
CTAGAGT4700.015.7446794
GGTTATC2150.015.4883742
CCGATCG2109.094947E-1214.9761918
CGATCGC2109.094947E-1214.9761919
ACACGCT3250.014.89
GTATTAG3250.014.81
CTTACTC750.004105502314.83
ATTAACC2900.014.6724153
TACCGTC2151.2732926E-1114.6279087
TACTTAC2800.014.53571431
CAAGACG3700.014.54
CGCCCGA3700.014.526
TCTAGAT2301.8189894E-1214.4782612
Produced by FastQC (version 0.11.5)