##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727115.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1715837
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.328621541556686	31.0	30.0	31.0	27.0	34.0
2	30.696134889269786	31.0	30.0	33.0	27.0	34.0
3	30.996935606354217	31.0	31.0	34.0	28.0	34.0
4	34.921089240994334	35.0	35.0	37.0	32.0	37.0
5	34.607206861724045	35.0	35.0	37.0	32.0	37.0
6	34.54355629351739	35.0	35.0	37.0	30.0	37.0
7	34.32831440282498	35.0	35.0	37.0	30.0	37.0
8	34.3877390451424	35.0	35.0	37.0	30.0	37.0
9	35.64485379438723	37.0	35.0	39.0	30.0	39.0
10	35.509748303597604	37.0	35.0	39.0	30.0	39.0
11	35.514849604012504	37.0	35.0	39.0	30.0	39.0
12	35.36984107464753	37.0	34.0	39.0	30.0	39.0
13	35.50872314794471	37.0	35.0	39.0	30.0	39.0
14	36.13634745025314	38.0	34.0	40.0	29.0	41.0
15	36.22034727074891	38.0	34.0	40.0	30.0	41.0
16	36.00427721281217	38.0	34.0	40.0	29.0	41.0
17	36.14969836878445	38.0	34.0	40.0	30.0	41.0
18	36.07491795549344	38.0	34.0	40.0	29.0	41.0
19	36.03355563494667	38.0	34.0	40.0	28.0	41.0
20	36.526000430110784	38.0	35.0	40.0	30.0	41.0
21	36.61848823635345	38.0	35.0	40.0	30.0	41.0
22	36.637051188428735	38.0	35.0	40.0	30.0	41.0
23	36.554011249320304	38.0	35.0	40.0	30.0	41.0
24	36.423724398063456	38.0	35.0	40.0	30.0	41.0
25	36.1067875328484	38.0	35.0	40.0	29.0	41.0
26	35.86190529753117	38.0	34.0	40.0	27.0	41.0
27	35.65924327310811	38.0	34.0	40.0	27.0	41.0
28	35.39855300940591	38.0	34.0	40.0	27.0	41.0
29	35.19884697672332	38.0	34.0	40.0	26.0	41.0
30	34.80534398080937	37.0	33.0	40.0	25.0	41.0
31	34.61818983971088	37.0	33.0	40.0	25.0	41.0
32	34.348214311732406	37.0	33.0	40.0	24.0	41.0
33	34.27549236903039	37.0	33.0	40.0	24.0	41.0
34	34.050242534692984	36.0	33.0	40.0	24.0	41.0
35	33.62369735586772	36.0	32.0	40.0	22.0	41.0
36	33.607772183488294	36.0	32.0	40.0	22.0	41.0
37	33.40664119027623	36.0	32.0	39.0	21.0	41.0
38	33.23446108225898	36.0	31.0	39.0	20.0	41.0
39	33.028245690004354	36.0	31.0	39.0	18.0	41.0
40	32.70495507440392	35.0	31.0	39.0	16.0	40.0
41	32.3745046877996	35.0	30.0	39.0	15.0	40.0
42	32.07645423195793	35.0	30.0	39.0	15.0	40.0
43	31.766126968937026	35.0	30.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	7.0
11	5.0
12	9.0
13	13.0
14	33.0
15	75.0
16	137.0
17	265.0
18	571.0
19	1102.0
20	2182.0
21	3891.0
22	6522.0
23	10255.0
24	14904.0
25	21062.0
26	28401.0
27	37356.0
28	47901.0
29	59226.0
30	72238.0
31	87819.0
32	105104.0
33	124745.0
34	145906.0
35	166769.0
36	195203.0
37	222748.0
38	226086.0
39	135299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.503329278946666	23.80062908073436	15.784191621931454	22.91185001838753
2	17.853968646205903	28.43014808516194	29.629387873090508	24.08649539554165
3	21.303888422967916	27.779620092118307	30.79983704745847	20.116654437455306
4	16.87992507446803	18.76996474606854	29.508047675857323	34.842062503606115
5	13.1706566532835	42.36806876177632	30.391348362344438	14.069926222595736
6	32.83616101063213	36.089267220604285	14.873382494957271	16.20118927380631
7	25.849774774643514	34.65270885288055	20.247844055117124	19.24967231735882
8	30.03152397343104	34.471281362973286	19.69009876812308	15.807095895472589
9	29.292001512964227	14.344544382712343	19.54591257794301	36.817541526380424
10	19.108050473325846	31.371977641232824	29.481238602501286	20.038733282940047
11	33.158219574470074	25.06112177322205	18.688138791738375	23.092519860569507
12	20.638557158984216	32.67658874356946	27.614161485036163	19.070692612410152
13	30.387327001341035	21.079566415691	26.51190060594334	22.021205977024625
14	22.617999262167675	24.027340592375616	27.03794124966416	26.31671889579255
15	26.805984484540197	29.02641684495672	22.68589615447155	21.481702516031532
16	21.039003122091433	27.797162550988237	27.95825011350146	23.205584213418874
17	22.046907719089866	31.049744235612124	26.372260302114945	20.531087743183065
18	22.16865588048282	25.928337015695547	29.50251101940336	22.400496084418275
19	23.135938903287435	29.0258340390142	28.606097199209486	19.23212985848889
20	22.629014294481355	27.016668832762086	29.64908671394777	20.705230158808792
21	22.693239509347332	29.183657888249293	28.6208421895553	19.502260412848074
22	22.79517226869452	29.114245700494862	26.15539821090232	21.935183819908303
23	20.80046064981697	28.983114363427294	28.223834781508966	21.992590205246767
24	20.466104880591804	31.68651800841222	27.449518806273552	20.39785830472242
25	21.550357056060687	29.826842526417135	26.30920069913401	22.31359971838817
26	21.63253269395636	29.786920319354344	27.96996451294616	20.610582473743136
27	21.946198852221976	30.02960071382072	26.692279045154056	21.33192138880325
28	20.5475228707622	30.74919121105326	28.686407858089087	20.016878060095454
29	21.301732040980585	29.499655270284997	28.821851959131315	20.376760729603102
30	20.42898014205312	31.807158838514383	28.637918403671208	19.12594261576129
31	21.687258171959225	30.6932418405711	26.68202166056566	20.937478326904014
32	18.605496909088686	32.087080532707944	27.866050213394395	21.441372344808975
33	19.631701612682324	31.013726828364234	28.70132769021766	20.65324386873578
34	19.192732176774367	31.07480489114059	28.41575277838163	21.31671015370341
35	19.49101225815739	32.12945052472933	28.60032742037851	19.77920979673477
36	20.224764939793232	31.444012455728604	27.424632992527847	20.90658961195032
37	19.210566038615557	31.789150134890438	28.286078456170372	20.714205370323636
38	20.16566841722145	30.954805147575204	28.54647615128943	20.333050283913913
39	20.059714296870858	29.540043722101807	28.547758324362977	21.852483656664358
40	19.710963220865384	30.867733939762342	29.00240524012479	20.418897599247483
41	18.666633252459295	31.08949160089216	28.87686884010544	21.367006306543104
42	19.55034190310618	31.60579938537285	28.941152335565672	19.902706375955294
43	19.385582663155066	29.90575445103468	29.13132191461077	21.57734097119948
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	794.0
1	1318.5
2	1843.0
3	1847.0
4	1851.0
5	1851.0
6	2385.0
7	2919.0
8	4474.0
9	6029.0
10	10075.5
11	14122.0
12	14122.0
13	28852.5
14	43583.0
15	55344.0
16	67105.0
17	51536.0
18	35967.0
19	35967.0
20	39247.0
21	42527.0
22	36229.0
23	29931.0
24	30676.0
25	31421.0
26	31421.0
27	33310.0
28	35199.0
29	35724.5
30	36250.0
31	37898.5
32	39547.0
33	39547.0
34	44311.0
35	49075.0
36	55003.0
37	60931.0
38	67875.5
39	74820.0
40	74820.0
41	80124.0
42	85428.0
43	92631.5
44	99835.0
45	116146.5
46	132458.0
47	132458.0
48	163937.5
49	195417.0
50	186033.5
51	176650.0
52	141099.0
53	105548.0
54	105548.0
55	94331.5
56	83115.0
57	77804.5
58	72494.0
59	66259.0
60	60024.0
61	60024.0
62	50922.5
63	41821.0
64	36004.5
65	30188.0
66	25364.5
67	20541.0
68	20541.0
69	16741.5
70	12942.0
71	11505.5
72	10069.0
73	7973.0
74	5877.0
75	5877.0
76	4583.0
77	3289.0
78	2828.0
79	2367.0
80	1828.5
81	1290.0
82	1290.0
83	1099.0
84	908.0
85	736.5
86	565.0
87	570.0
88	575.0
89	575.0
90	410.0
91	245.0
92	193.0
93	141.0
94	122.5
95	104.0
96	104.0
97	56.5
98	9.0
99	16.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1715837.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.80511517845907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.89027599822823	45.013607559763116
2	6.910206566151427	7.159680941328375
3	2.194239499704875	3.410184900340063
4	1.0843587563030048	2.247013210601912
5	0.6659547459507479	1.7249931158809972
6	0.40873306078647864	1.2704677974772582
7	0.30532671064662253	1.1072239788475828
8	0.2226508615057004	0.9227562819908757
9	0.17270264188703588	0.8052192229123857
>10	0.9462891890376777	9.185578487146746
>50	0.09892564030536144	3.599501475268813
>100	0.08376509696174624	8.722800947197713
>500	0.009761136970373155	3.4466579595775713
>1k	0.005788581226616639	5.558799498141229
>5k	6.810095560725456E-4	2.3100610958923866
>10k+	3.405047780362728E-4	3.515453527633012
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29573	1.7235320138218257	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18466	1.0762094534620712	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11748	0.6846804212754475	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	8430	0.49130540954647794	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	7594	0.4425828327515958	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6586	0.3838359937453266	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	6071	0.3538214877054172	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	5364	0.3126171075690756	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5242	0.30550687507030094	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	4885	0.2847007029222473	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	4558	0.26564294860176113	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4267	0.24868329567435601	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	4017	0.2341131471112932	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3537	0.20613846187021262	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	3076	0.1792711079199248	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	2669	0.15555090605925856	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2624	0.15292827931790723	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	2433	0.14179668581572727	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	2348	0.1368428353042859	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	2336	0.1361434681732589	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2219	0.12932463864574548	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	2175	0.12676029249864645	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	2079	0.12116535545043032	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	2068	0.12052426891365556	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1783	0.10391429955176396	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1773	0.10333149360924143	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.828059425225123E-5
2	0.0	0.0	0.0	0.0	5.828059425225123E-5
3	0.0	0.0	0.0	0.0	5.828059425225123E-5
4	0.0	0.0	0.0	1.1656118850450246E-4	5.828059425225123E-5
5	0.0	0.0	0.0	1.1656118850450246E-4	5.828059425225123E-5
6	0.0	0.0	0.0	1.1656118850450246E-4	5.828059425225123E-5
7	0.0	0.0	0.0	1.1656118850450246E-4	5.828059425225123E-5
8	0.0	0.0	0.0	1.1656118850450246E-4	5.828059425225123E-5
9	0.0	0.0	0.0	1.1656118850450246E-4	1.1656118850450246E-4
10	0.0	0.0	0.0	1.748417827567537E-4	1.1656118850450246E-4
11	0.0	0.0	0.0	2.9140297126125617E-4	1.1656118850450246E-4
12	0.0	0.0	0.0	4.6624475401800986E-4	1.1656118850450246E-4
13	0.0	0.0	0.0	5.245253482702611E-4	1.1656118850450246E-4
14	0.0	0.0	0.0	0.0010490506965405222	1.1656118850450246E-4
15	0.0	0.0	0.0	0.0023895043643423004	1.1656118850450246E-4
16	0.0	0.0	0.0	0.006352584773495385	1.1656118850450246E-4
17	0.0	0.0	0.0	0.011656118850450247	1.1656118850450246E-4
18	0.0	0.0	0.0	0.013812500837783542	1.1656118850450246E-4
19	0.0	0.0	0.0	0.019640560263008665	1.1656118850450246E-4
20	0.0	0.0	0.0	0.02552690028248604	1.1656118850450246E-4
21	0.0	0.0	0.0	0.04050501300531461	1.1656118850450246E-4
22	0.0	0.0	0.0	0.06352584773495384	1.1656118850450246E-4
23	0.0	0.0	0.0	0.10542959500232248	1.748417827567537E-4
24	0.0	0.0	0.0	0.18504088675089767	1.748417827567537E-4
25	0.0	0.0	0.0	0.22758572055504106	1.748417827567537E-4
26	0.0	0.0	0.0	0.27817327636599515	1.748417827567537E-4
27	0.0	0.0	0.0	0.3485179536284624	1.748417827567537E-4
28	5.828059425225123E-5	0.0	0.0	0.46338900489964957	1.748417827567537E-4
29	5.828059425225123E-5	0.0	0.0	0.5779103726053232	1.748417827567537E-4
30	5.828059425225123E-5	0.0	0.0	0.7609697191516444	1.748417827567537E-4
31	5.828059425225123E-5	0.0	0.0	1.0990554464089537	1.748417827567537E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGACT	130	6.9667294E-10	18.5	4
TTAGAGT	200	0.0	17.575	4
AGGTTAT	200	0.0	17.575	1
TAGAGTG	215	0.0	17.209303	5
TATCTAG	305	0.0	16.37705	1
AAGACGG	335	0.0	16.014925	5
CGAACGT	105	9.34839E-6	15.857142	4
CTAGAGT	470	0.0	15.744679	4
GGTTATC	215	0.0	15.488374	2
CCGATCG	210	9.094947E-12	14.97619	18
CGATCGC	210	9.094947E-12	14.97619	19
ACACGCT	325	0.0	14.8	9
GTATTAG	325	0.0	14.8	1
CTTACTC	75	0.0041055023	14.8	3
ATTAACC	290	0.0	14.672415	3
TACCGTC	215	1.2732926E-11	14.627908	7
TACTTAC	280	0.0	14.535714	31
CAAGACG	370	0.0	14.5	4
CGCCCGA	370	0.0	14.5	26
TCTAGAT	230	1.8189894E-12	14.478261	2
>>END_MODULE