FastQCFastQC Report
Thu 9 Feb 2017
SRR2727114.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727114.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402897
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT248531.034293188596931No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147700.6146747030771605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT97150.40430363848304773No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG59020.2456201826378742No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC57170.2379211426873478No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56890.2367558825867276No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA42170.1754964944398366No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG41750.17374860428890626No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39330.16367742770497445No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG35780.1489035942863968No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT34770.14470033463773105No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33870.14095485574288036No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27810.11573529785088583No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG26470.11015869594077482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC509.09648E-625.8999983
TCGAACG1551.8189894E-1219.0967753
TAAGACT2950.017.5593224
AAGACGG5900.017.2457625
CGAACGT1757.2759576E-1216.9142864
GTATTAG4950.016.0707071
CAAGACG6600.015.9772744
CCGTCGT4700.014.9574479
ACCGTCG4850.014.8762888
ATACCGT5150.014.7281566
TACCGTC4900.014.7244897
TTAGAGT3650.014.6986294
CGTCGTA5100.014.147058510
ATTCGAA2101.364242E-1014.0952381
ATTAGAG4100.013.9878043
GGGTAGG6750.013.9777781
CGAACGA2650.013.96226416
ACACGCT5050.013.9207939
GCGATCT800.006301256413.87500128
CACTCTA4150.013.8192779
Produced by FastQC (version 0.11.5)