##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727113.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2160749
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.274369674589693	31.0	30.0	31.0	27.0	34.0
2	30.654205092771072	31.0	30.0	33.0	27.0	34.0
3	30.97818256539746	31.0	31.0	34.0	28.0	34.0
4	34.89270294698736	35.0	35.0	37.0	32.0	37.0
5	34.57014674078294	35.0	35.0	37.0	32.0	37.0
6	34.51566979783399	35.0	35.0	37.0	30.0	37.0
7	34.317516287176346	35.0	35.0	37.0	30.0	37.0
8	34.35932354938033	35.0	35.0	37.0	30.0	37.0
9	35.65779782843819	37.0	35.0	39.0	30.0	39.0
10	35.512559302353026	37.0	35.0	39.0	30.0	39.0
11	35.521320153335715	37.0	35.0	39.0	30.0	39.0
12	35.363981193558345	37.0	34.0	39.0	30.0	39.0
13	35.49112691941544	37.0	35.0	39.0	30.0	39.0
14	36.14714110708833	38.0	34.0	40.0	30.0	41.0
15	36.19550859447349	38.0	34.0	40.0	30.0	41.0
16	35.994704614001904	38.0	34.0	40.0	29.0	41.0
17	36.110678287945525	38.0	34.0	40.0	29.0	41.0
18	36.005905822471746	38.0	34.0	40.0	29.0	41.0
19	35.95265252928498	38.0	34.0	40.0	27.0	41.0
20	36.4477012369322	38.0	35.0	40.0	30.0	41.0
21	36.53949301839316	38.0	35.0	40.0	30.0	41.0
22	36.57454706678101	38.0	35.0	40.0	30.0	41.0
23	36.4762991906973	38.0	35.0	40.0	30.0	41.0
24	36.35951005878054	38.0	35.0	40.0	30.0	41.0
25	36.062913369391815	38.0	34.0	40.0	29.0	41.0
26	35.81551165822592	38.0	34.0	40.0	27.0	41.0
27	35.64193943859282	38.0	34.0	40.0	27.0	41.0
28	35.38644377482068	38.0	34.0	40.0	27.0	41.0
29	35.204767883729204	38.0	34.0	40.0	26.0	41.0
30	34.830602721556275	37.0	33.0	40.0	25.0	41.0
31	34.654750274094766	37.0	33.0	40.0	25.0	41.0
32	34.3932279964031	37.0	33.0	40.0	24.0	41.0
33	34.36035976413734	37.0	33.0	40.0	25.0	41.0
34	34.13722001028347	37.0	33.0	40.0	24.0	41.0
35	33.7359781261035	37.0	32.0	40.0	23.0	41.0
36	33.746237994325114	36.0	32.0	40.0	23.0	41.0
37	33.559626777566486	36.0	32.0	40.0	21.0	41.0
38	33.43123102220573	36.0	32.0	40.0	21.0	41.0
39	33.26835092831236	36.0	31.0	40.0	20.0	41.0
40	32.992690034798116	36.0	31.0	39.0	18.0	41.0
41	32.72539013092219	36.0	31.0	39.0	16.0	40.0
42	32.4645953787321	36.0	30.0	39.0	15.0	40.0
43	32.18836246135021	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	14.0
12	17.0
13	18.0
14	36.0
15	73.0
16	175.0
17	346.0
18	676.0
19	1434.0
20	2665.0
21	4758.0
22	8002.0
23	12387.0
24	18277.0
25	26088.0
26	35148.0
27	46794.0
28	59512.0
29	73860.0
30	90613.0
31	110287.0
32	131141.0
33	155320.0
34	182033.0
35	209190.0
36	246051.0
37	280689.0
38	294784.0
39	170353.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.79168658645683	23.81766692938421	15.483380994275597	23.90726548988337
2	17.448718013984966	28.367293008118942	29.734550380446777	24.449438597449312
3	20.984390135087416	27.85686815081252	30.556672709324406	20.602069004775657
4	17.0535772549241	19.105504619000172	29.781015749631262	34.05990237644446
5	13.320519875283987	41.53735579653166	31.190434428061753	13.951689900122597
6	33.0324345863402	36.05402571052908	14.947779681952877	15.965760021177841
7	25.821555395837276	33.82600200208354	20.864801973760024	19.487640628319163
8	29.240416170503842	34.95093599487955	20.116496640748185	15.692151193868423
9	28.756741296652226	14.441196085246366	19.56020805748377	37.24185456061764
10	18.660520032636832	30.769746971999062	30.391868745513705	20.1778642498504
11	33.24226923164144	24.696158600559343	19.10388481031346	22.957687357485764
12	20.491875733831186	31.775277924460454	28.217298723729595	19.515547617978765
13	30.623917909946968	20.892894084412397	26.093960936693712	22.389227068946926
14	22.627986869367984	23.47722942368595	26.60109989637852	27.293683810567536
15	26.939223389667195	28.366367403155106	22.53639825819658	22.158010948981115
16	21.24004222609845	27.606029205613424	28.063231777499375	23.09069679078875
17	22.57689347536433	30.673391495263914	26.42227301736574	20.32744201200602
18	23.070842564314503	25.31168590150915	29.042799510725214	22.57467202345113
19	23.4590875663948	29.37166695437554	27.72899582505881	19.440249654170845
20	22.702590629453027	26.94121344033944	29.17511474030533	21.181081189902205
21	22.25899445053544	29.287853424900344	28.0860016596097	20.367150464954513
22	22.94658009791975	28.998463032957556	25.2075090628296	22.847447806293093
23	20.784366902402823	28.63544076614174	27.327560952243875	23.25263137921156
24	20.767752293302	31.788467795195093	26.650342080454507	20.7934378310484
25	22.204707719406557	29.1445697764988	25.27429146097025	23.3764310431244
26	21.992790462936696	29.160767863365898	27.43562533177153	21.41081634192588
27	22.98304893349482	29.26767523668876	25.74075008249454	22.008525747321876
28	21.115826039951887	29.919254850979915	28.053836887116457	20.91108222195174
29	21.796515930355632	28.787980464181633	28.310969946069626	21.10453365939311
30	20.881138901371703	31.093060785866385	28.3848332221836	19.64096709057831
31	22.619239902459746	29.594552629666843	25.84464923968494	21.941558228188466
32	18.870586079179024	31.116802553188734	27.43456088606312	22.57805048156912
33	20.442170747273284	30.120435089869304	28.182079454855703	21.255314708001716
34	19.827522771039117	30.131357228442546	27.814660564461676	22.226459436056665
35	20.105389381182174	31.36282835257589	28.38367621597881	20.148106050263127
36	21.407576724552456	30.20952456763835	26.768125312102427	21.614773395706766
37	20.066837934438475	30.7214766731351	27.77310090158551	21.438584490840906
38	21.050478329505186	29.837431372176965	28.21489215082363	20.897198147494226
39	20.990637968593298	28.039490010177026	28.059668198388614	22.910203822841062
40	20.493912064751623	29.700719519018637	28.62326906086732	21.182099355362425
41	19.531005220874796	29.732606610022728	28.40825102776861	22.328137141333862
42	20.558519291227256	30.440740687604162	28.61739146934697	20.383348551821616
43	20.525567754514753	28.435324972960764	28.736054025710526	22.303053246813953
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	861.0
1	1348.5
2	1836.0
3	1875.0
4	1914.0
5	1914.0
6	2508.5
7	3103.0
8	4973.0
9	6843.0
10	10768.0
11	14693.0
12	14693.0
13	29727.5
14	44762.0
15	56796.5
16	68831.0
17	52978.5
18	37126.0
19	37126.0
20	41166.5
21	45207.0
22	39643.0
23	34079.0
24	34642.0
25	35205.0
26	35205.0
27	38254.5
28	41304.0
29	42961.0
30	44618.0
31	46820.5
32	49023.0
33	49023.0
34	55088.0
35	61153.0
36	68661.5
37	76170.0
38	84330.0
39	92490.0
40	92490.0
41	99650.5
42	106811.0
43	117128.0
44	127445.0
45	149591.0
46	171737.0
47	171737.0
48	225139.5
49	278542.0
50	264825.0
51	251108.0
52	194023.0
53	136938.0
54	136938.0
55	120421.5
56	103905.0
57	97020.5
58	90136.0
59	81821.5
60	73507.0
61	73507.0
62	62573.5
63	51640.0
64	44518.0
65	37396.0
66	31551.0
67	25706.0
68	25706.0
69	20940.5
70	16175.0
71	14274.5
72	12374.0
73	9820.0
74	7266.0
75	7266.0
76	5559.0
77	3852.0
78	3234.0
79	2616.0
80	2072.5
81	1529.0
82	1529.0
83	1276.0
84	1023.0
85	839.0
86	655.0
87	634.5
88	614.0
89	614.0
90	437.5
91	261.0
92	198.0
93	135.0
94	128.5
95	122.0
96	122.0
97	68.5
98	15.0
99	19.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2160749.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.33869949061463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.82856775290475	42.34669912070425
2	7.261063680242295	7.165028778033819
3	2.3590681376914246	3.4918006177032335
4	1.2168226943476916	2.401457969991231
5	0.7484787641664937	1.8464484410158626
6	0.47833846822189635	1.4160358763040637
7	0.33548764911370554	1.1586767011703718
8	0.25587570975410734	1.0099659800404508
9	0.191333398477687	0.8496126945007759
>10	1.1154162777017276	10.17546507286268
>50	0.10619589292463674	3.631197739432986
>100	0.08688464412783752	8.812670819433894
>500	0.008706652815843301	2.9373119481905814
>1k	0.006719264673096461	6.6090569425141785
>5k	4.73187653034962E-4	1.6366804712985243
>10k+	5.678251836419544E-4	4.511890826803181
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29639	1.371700276154241	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18545	0.8582672027153548	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	13966	0.6463499462454917	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	13317	0.6163140651690687	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10961	0.5072778004293881	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	10201	0.4721048118036847	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	8269	0.3826913722972914	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	7305	0.3380772130404781	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	6763	0.31299331852056855	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	6738	0.3118363123157757	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5977	0.2766170434418806	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4962	0.22964259152728983	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	4747	0.21969233816607112	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	4277	0.19794062151596506	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4137	0.19146138676912497	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	4107	0.1900729793233735	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	3921	0.18146485315971453	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	3855	0.17841035677906134	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	3648	0.16883034540337633	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3534	0.1635543971095208	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	2801	0.12963097518499372	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	2716	0.12569715408869794	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	2706	0.1252343516067808	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	2696	0.12477154912486366	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	2686	0.12430874664294651	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	2657	0.12296661944538677	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	2518	0.11653366494673839	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	2478	0.11468245501906978	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	2445	0.11315520682874318	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	2405	0.11130399690107459	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	2349	0.10871230300233854	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	2319	0.10732389555658708	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.6280248191715E-5	0.0
5	0.0	0.0	0.0	4.6280248191715E-5	0.0
6	0.0	0.0	0.0	4.6280248191715E-5	0.0
7	0.0	0.0	0.0	4.6280248191715E-5	0.0
8	0.0	0.0	0.0	9.256049638343E-5	0.0
9	0.0	0.0	0.0	1.38840744575145E-4	0.0
10	0.0	0.0	0.0	2.7768148915029E-4	0.0
11	0.0	0.0	0.0	3.7024198553372E-4	0.0
12	0.0	0.0	0.0	5.090827301088651E-4	0.0
13	0.0	0.0	0.0	8.79324715642585E-4	0.0
14	0.0	0.0	0.0	0.00166608893490174	9.256049638343E-5
15	0.0	0.0	0.0	0.002637974146927755	9.256049638343E-5
16	0.0	0.0	0.0	0.00592387176853952	1.38840744575145E-4
17	0.0	0.0	0.0	0.00971885212026015	1.38840744575145E-4
18	0.0	0.0	0.0	0.01157006204792875	1.8512099276686E-4
19	0.0	0.0	0.0	0.016614609100825687	1.8512099276686E-4
20	0.0	0.0	0.0	0.02175171665010605	1.8512099276686E-4
21	0.0	0.0	0.0	0.03387714167633538	1.8512099276686E-4
22	0.0	0.0	0.0	0.05470325336260713	1.8512099276686E-4
23	0.0	0.0	0.0	0.08696058635223249	1.8512099276686E-4
24	0.0	0.0	0.0	0.14754143123518743	1.8512099276686E-4
25	0.0	0.0	0.0	0.18151113340790623	1.8512099276686E-4
26	0.0	0.0	0.0	0.2261252926647195	1.8512099276686E-4
27	0.0	0.0	0.0	0.285317730101923	1.8512099276686E-4
28	0.0	0.0	0.0	0.39560356154277987	1.8512099276686E-4
29	0.0	0.0	0.0	0.5017241706463823	1.8512099276686E-4
30	0.0	0.0	0.0	0.6666206949534629	1.8512099276686E-4
31	0.0	0.0	0.0	0.9603151499780863	1.8512099276686E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTATA	85	2.7249127E-5	17.411764	1
CAAGACG	475	0.0	17.136843	4
AAGACGG	450	0.0	16.444445	5
TAATACC	195	1.8189894E-12	16.128206	4
CGCCCGA	460	0.0	16.086956	26
CGCAAGA	495	0.0	16.070707	2
TATCTAG	485	0.0	16.020618	1
TAGACTC	190	2.7284841E-11	15.578948	5
GCCCGAC	495	0.0	15.323233	27
TAGAGTC	535	0.0	15.214952	5
CATTACG	135	3.9765473E-7	15.074075	24
AAAAAGT	1095	0.0	15.0365305	1
AAAAGTA	1135	0.0	14.832601	2
ACGGACC	490	0.0	14.724489	8
AGGTTAT	315	0.0	14.682539	1
CTAGAGT	610	0.0	14.557377	4
TTATCTA	280	0.0	14.535715	4
TAGGGTG	230	1.8189894E-12	14.478261	5
AGTACTC	1280	0.0	14.453125	5
TACACCG	90	8.278168E-4	14.388889	5
>>END_MODULE