FastQCFastQC Report
Thu 9 Feb 2017
SRR2727112.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727112.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2836578
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT280980.990559751926441No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181760.640772085237917No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG153700.5418500742796426No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC138800.48932199290835654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT112400.3962521037672858No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA99060.34922360675433567No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG82600.2911959410247136No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT77200.2721589182458582No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG74910.26408581043778806No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG67970.23961971079237027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64660.22795072090384963No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC55260.1948121997702866No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC49950.17609246070441215No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44380.1564561242454817No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA43150.15211991350140908No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT42710.15056874868239126No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG41610.14669083663484664No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38920.13720757899130573No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC37960.1338232193861759No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34950.12321184187425835No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC29630.10445684906249712No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT29450.10382228163653529No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT28760.10138977317034822No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG852.7253996E-517.4117645
CGCTTCG4600.016.4891332
AAGACGG6300.016.4444455
GTTGCGA803.3847563E-416.187535
CGTCGTA4500.016.03333310
TACCGTC5100.015.9607847
CGAACGA2900.015.94827616
CAAGACG6650.015.8571434
GGTTATC3900.015.6538472
GTAAACG4750.015.57894727
TCTAGAT5050.015.3861382
CTAGATA4250.015.2352943
CACTCTA5250.015.1523829
AGTACTC12400.015.0685495
AAACGCT5050.015.01980129
ACGCTTC5050.015.01980131
ACCGTCG5050.015.0198018
ATACCGT5300.015.0094346
TATCTAG5950.014.9243691
CGATAAC3100.014.91935510
Produced by FastQC (version 0.11.5)