##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727112.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2836578
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.403625424719504	31.0	30.0	31.0	27.0	34.0
2	30.78868446416774	31.0	31.0	33.0	27.0	34.0
3	31.118893963078047	31.0	31.0	34.0	28.0	34.0
4	35.022147813315904	35.0	35.0	37.0	32.0	37.0
5	34.721825030018564	35.0	35.0	37.0	32.0	37.0
6	34.63669216922644	35.0	35.0	37.0	31.0	37.0
7	34.45770361329743	35.0	35.0	37.0	30.0	37.0
8	34.48183480235692	35.0	35.0	37.0	30.0	37.0
9	35.80506723241878	37.0	35.0	39.0	30.0	39.0
10	35.654987453191836	37.0	35.0	39.0	30.0	39.0
11	35.65164680823161	37.0	35.0	39.0	30.0	39.0
12	35.486922622963306	37.0	35.0	39.0	30.0	39.0
13	35.59756051129213	37.0	35.0	39.0	30.0	39.0
14	36.309263838329144	38.0	35.0	40.0	30.0	41.0
15	36.32682443423026	38.0	35.0	40.0	30.0	41.0
16	36.1400856948055	38.0	34.0	40.0	29.0	41.0
17	36.177717658389795	38.0	34.0	40.0	30.0	41.0
18	36.05820499207143	38.0	34.0	40.0	29.0	41.0
19	35.9817783963635	38.0	34.0	40.0	27.0	41.0
20	36.48001535653171	38.0	35.0	40.0	30.0	41.0
21	36.57331122218391	38.0	35.0	40.0	30.0	41.0
22	36.5867460722039	38.0	35.0	40.0	30.0	41.0
23	36.49909679903038	38.0	35.0	40.0	30.0	41.0
24	36.36848872126908	38.0	35.0	40.0	30.0	41.0
25	36.066235795384436	38.0	34.0	40.0	29.0	41.0
26	35.83480764498632	38.0	34.0	40.0	27.0	41.0
27	35.662661136059015	38.0	34.0	40.0	27.0	41.0
28	35.40633502762836	38.0	34.0	40.0	27.0	41.0
29	35.231974935996824	38.0	34.0	40.0	26.0	41.0
30	34.8751146628085	38.0	33.0	40.0	25.0	41.0
31	34.719215900285484	38.0	33.0	40.0	25.0	41.0
32	34.488565800059085	37.0	33.0	40.0	24.0	41.0
33	34.44735276096762	37.0	33.0	40.0	24.0	41.0
34	34.24833760961271	37.0	33.0	40.0	24.0	41.0
35	33.854673130793515	37.0	32.0	40.0	23.0	41.0
36	33.84174734486413	37.0	32.0	40.0	23.0	41.0
37	33.67672103499358	37.0	32.0	40.0	22.0	41.0
38	33.551251895770186	37.0	32.0	40.0	21.0	41.0
39	33.396337417832335	37.0	31.0	40.0	20.0	41.0
40	33.14869219178884	36.0	31.0	40.0	18.0	41.0
41	32.91645214762295	36.0	31.0	40.0	17.0	41.0
42	32.70103166561963	36.0	30.0	39.0	15.0	41.0
43	32.479844375864154	36.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	9.0
11	19.0
12	18.0
13	20.0
14	69.0
15	118.0
16	218.0
17	410.0
18	874.0
19	1803.0
20	3443.0
21	6326.0
22	10314.0
23	16414.0
24	23796.0
25	33426.0
26	44933.0
27	59418.0
28	75643.0
29	95204.0
30	116096.0
31	140315.0
32	168240.0
33	198946.0
34	233699.0
35	269091.0
36	314889.0
37	363610.0
38	401092.0
39	258123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.79778944911792	23.060885334371203	15.000116337361428	25.141208879149453
2	17.897410189319665	26.529994944612838	30.699984276829333	24.87261058923816
3	20.274958065669267	26.898114559162483	30.478802275135745	22.348125100032505
4	16.353613403192156	18.203165927395617	30.895466297771467	34.54775437164076
5	13.22015470753845	41.02524238712984	31.915956479955774	13.838646425375927
6	33.64991197139652	36.39723638835245	14.763281672494111	15.189569967756924
7	26.83627948887709	33.264447513870586	20.73463165828685	19.16464133896547
8	28.958731259990028	35.37015375568731	19.48414603793726	16.1869689463854
9	28.576333878356248	14.369603092176558	18.938700081577167	38.11536294789003
10	17.920007840433087	30.015920591642463	31.134416187391988	20.92965538053246
11	34.72303599618978	23.846233031490762	19.277136042090152	22.15359493022931
12	21.013841325710064	29.900711350084503	28.50314710189531	20.58230022231012
13	30.7283282885223	20.516551986231296	25.4072688993569	23.347850825889505
14	22.805013646725033	22.628533394815868	25.765446957566475	28.801006000892627
15	26.823588140357852	28.037938671173507	22.071947254755553	23.066525933713088
16	22.76334371908687	26.94930299819007	26.681163006975307	23.60619027574775
17	23.458300811752753	29.185236577312523	25.61470194015465	21.741760670780074
18	23.913708701118036	25.056247351562337	27.781220893626053	23.24882305369357
19	24.444383337951574	28.025811382588454	26.73749849290236	20.792306786557603
20	23.537022426317908	25.9423150006804	27.9327062397015	22.587956333300195
21	23.361811309260666	28.05775127636187	26.995732181522946	21.584705232854517
22	23.791942262825135	27.497005194286917	24.88829850615777	23.822754036730174
23	21.95860646172959	27.328033990251633	26.455821063267077	24.2575384847517
24	22.13004542797695	29.74845042159955	25.923912545327504	22.197591605095997
25	23.301456896302515	27.67436678984325	24.717282584861056	24.306893728993174
26	23.168374005579963	27.642497403561617	26.484764388640116	22.704364202218308
27	23.976671891271806	27.782772058445072	25.11078489644917	23.12977115383395
28	22.355422625431064	28.384377232002787	26.837689638712565	22.422510503853587
29	22.965523951747493	27.53359858251739	27.098532104528765	22.402345361206354
30	22.34082757463394	29.182557292625127	27.194563308324327	21.282051824416605
31	23.673172392932614	27.928616805178635	25.248168744169913	23.150042057718842
32	20.734173359590326	29.137185721668857	26.423986930731324	23.704653988009497
33	21.860601048164373	28.252034669943853	27.0781906931521	22.80917358873967
34	21.57800702113603	28.42872644432834	26.89307327350068	23.100193261034953
35	21.93766573667285	29.309999584005798	27.210850538924014	21.541484140397337
36	22.77850282981818	28.539246937683366	26.057453734746584	22.624796497751866
37	21.730549979588083	28.8106655272656	26.866068904151412	22.592715588994906
38	22.514099735667415	28.053238796888362	27.115136618841433	22.317524848602787
39	22.389371982720025	26.72671084666101	27.11115294555623	23.772764225062733
40	22.18458297286378	27.957242846838692	27.56574999876612	22.29242418153141
41	21.144773737933527	28.031734011897434	27.558699249588763	23.264793000580276
42	22.15810740970282	28.58839065944952	27.66382591982311	21.58967601102455
43	21.950251323954426	26.828347396052564	27.78365340209224	23.437747877900765
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	856.0
1	1229.0
2	1602.0
3	1622.0
4	1642.0
5	1642.0
6	2168.5
7	2695.0
8	4172.5
9	5650.0
10	9395.0
11	13140.0
12	13140.0
13	27090.5
14	41041.0
15	51948.0
16	62855.0
17	48882.5
18	34910.0
19	34910.0
20	38799.5
21	42689.0
22	38346.5
23	34004.0
24	36401.5
25	38799.0
26	38799.0
27	42782.5
28	46766.0
29	50011.5
30	53257.0
31	57215.0
32	61173.0
33	61173.0
34	69997.5
35	78822.0
36	90269.0
37	101716.0
38	114779.0
39	127842.0
40	127842.0
41	136846.0
42	145850.0
43	158020.0
44	170190.0
45	195113.5
46	220037.0
47	220037.0
48	280923.5
49	341810.0
50	331494.5
51	321179.0
52	260804.0
53	200429.0
54	200429.0
55	181841.0
56	163253.0
57	153397.5
58	143542.0
59	131388.0
60	119234.0
61	119234.0
62	101424.0
63	83614.0
64	71904.0
65	60194.0
66	50769.0
67	41344.0
68	41344.0
69	33635.0
70	25926.0
71	23099.0
72	20272.0
73	16050.5
74	11829.0
75	11829.0
76	9140.0
77	6451.0
78	5499.0
79	4547.0
80	3535.5
81	2524.0
82	2524.0
83	2110.0
84	1696.0
85	1415.0
86	1134.0
87	1125.0
88	1116.0
89	1116.0
90	773.5
91	431.0
92	343.5
93	256.0
94	223.0
95	190.0
96	190.0
97	109.5
98	29.0
99	35.5
100	42.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2836578.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.63743212234307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.95288410165517	43.867487863964975
2	7.8273677914829936	8.08370346058635
3	2.5568841424323563	3.9609279404863855
4	1.3071889789662043	2.699995286897693
5	0.7543052509419484	1.9475193097520913
6	0.48728138837758805	1.509717577009728
7	0.35239838763375964	1.2737863475022984
8	0.2567410295530475	1.0605957989252774
9	0.19677197222727677	0.9144719423519023
>10	1.1380630329241952	10.67194280113867
>50	0.08501816977856377	3.0428278959974753
>100	0.07027473525420301	7.540133530710803
>500	0.008165283490507188	2.899745416370236
>1k	0.005832345350356785	5.604653281330452
>5k	4.803107935587941E-4	1.8483170952244197
>10k+	3.430791382562815E-4	3.07417445175117
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28098	0.990559751926441	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18176	0.640772085237917	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	15370	0.5418500742796426	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	13880	0.48932199290835654	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11240	0.3962521037672858	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	9906	0.34922360675433567	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	8260	0.2911959410247136	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	7720	0.2721589182458582	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	7491	0.26408581043778806	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	6797	0.23961971079237027	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6466	0.22795072090384963	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	5526	0.1948121997702866	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	4995	0.17609246070441215	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4438	0.1564561242454817	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	4315	0.15211991350140908	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	4271	0.15056874868239126	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	4161	0.14669083663484664	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3892	0.13720757899130573	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	3796	0.1338232193861759	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3495	0.12321184187425835	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	2963	0.10445684906249712	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	2945	0.10382228163653529	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	2876	0.10138977317034822	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.52537458867692E-5	3.52537458867692E-5	0.0	0.0	0.0
8	3.52537458867692E-5	3.52537458867692E-5	0.0	0.0	0.0
9	3.52537458867692E-5	3.52537458867692E-5	0.0	1.0576123766030759E-4	0.0
10	3.52537458867692E-5	3.52537458867692E-5	0.0	1.410149835470768E-4	0.0
11	3.52537458867692E-5	3.52537458867692E-5	0.0	1.76268729433846E-4	0.0
12	3.52537458867692E-5	3.52537458867692E-5	0.0	2.1152247532061518E-4	0.0
13	3.52537458867692E-5	3.52537458867692E-5	0.0	2.820299670941536E-4	0.0
14	3.52537458867692E-5	3.52537458867692E-5	0.0	5.993136800750764E-4	3.52537458867692E-5
15	3.52537458867692E-5	7.05074917735384E-5	0.0	0.0013396423436972296	3.52537458867692E-5
16	3.52537458867692E-5	7.05074917735384E-5	0.0	0.0022914934826399978	7.05074917735384E-5
17	3.52537458867692E-5	7.05074917735384E-5	0.0	0.003913165793431381	7.05074917735384E-5
18	3.52537458867692E-5	7.05074917735384E-5	0.0	0.005041285661807995	7.05074917735384E-5
19	3.52537458867692E-5	7.05074917735384E-5	0.0	0.0075795553656553775	7.05074917735384E-5
20	3.52537458867692E-5	7.05074917735384E-5	0.0	0.009342242659993838	7.05074917735384E-5
21	3.52537458867692E-5	7.05074917735384E-5	0.0	0.015511648190178447	7.05074917735384E-5
22	3.52537458867692E-5	7.05074917735384E-5	0.0	0.02467762212073844	1.0576123766030759E-4
23	3.52537458867692E-5	7.05074917735384E-5	0.0	0.04364413740782027	1.76268729433846E-4
24	3.52537458867692E-5	7.05074917735384E-5	0.0	0.0787568683110424	1.76268729433846E-4
25	3.52537458867692E-5	7.05074917735384E-5	0.0	0.10241213180106452	1.76268729433846E-4
26	3.52537458867692E-5	7.05074917735384E-5	0.0	0.13012157606806513	1.76268729433846E-4
27	3.52537458867692E-5	7.05074917735384E-5	0.0	0.171685742468566	1.76268729433846E-4
28	3.52537458867692E-5	7.05074917735384E-5	0.0	0.2391261583499555	1.76268729433846E-4
29	3.52537458867692E-5	7.05074917735384E-5	0.0	0.30787096282915544	1.76268729433846E-4
30	3.52537458867692E-5	7.05074917735384E-5	0.0	0.40774482492637254	1.76268729433846E-4
31	3.52537458867692E-5	7.05074917735384E-5	0.0	0.6156361644206505	1.76268729433846E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	85	2.7253996E-5	17.411764	5
CGCTTCG	460	0.0	16.48913	32
AAGACGG	630	0.0	16.444445	5
GTTGCGA	80	3.3847563E-4	16.1875	35
CGTCGTA	450	0.0	16.033333	10
TACCGTC	510	0.0	15.960784	7
CGAACGA	290	0.0	15.948276	16
CAAGACG	665	0.0	15.857143	4
GGTTATC	390	0.0	15.653847	2
GTAAACG	475	0.0	15.578947	27
TCTAGAT	505	0.0	15.386138	2
CTAGATA	425	0.0	15.235294	3
CACTCTA	525	0.0	15.152382	9
AGTACTC	1240	0.0	15.068549	5
AAACGCT	505	0.0	15.019801	29
ACGCTTC	505	0.0	15.019801	31
ACCGTCG	505	0.0	15.019801	8
ATACCGT	530	0.0	15.009434	6
TATCTAG	595	0.0	14.924369	1
CGATAAC	310	0.0	14.919355	10
>>END_MODULE