FastQCFastQC Report
Thu 9 Feb 2017
SRR2727111.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727111.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2844637
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT270790.9519316524393094No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179440.6308010477259489No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT116760.410456589012939No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG98320.3456328522760549No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC90330.31754490994808826No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA68370.24034701088398977No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT67490.2372534703021862No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG61180.2150713781758446No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT55590.19542036470734228No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG50800.1785816608586614No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45960.1615671876587417No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG41990.1476111011703778No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC38460.1352017849729157No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37290.13108878215392683No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA35330.1241986235853643No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC34880.12261669942421474No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33950.11934738949117232No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG33320.11713269566556296No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC29000.10194622371852717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT1700.025.0294134
TCGAACG1850.023.03
ATTCGAA2250.018.9111121
TACCGTA500.007037369518.57
AAGACGG7100.015.6338025
CAAGACG6950.015.43884854
GAACGTC2800.015.1964285
ACCGTAT750.004106385614.88
TATCTAG5750.014.4782611
GTGCGTA2301.8189894E-1214.47826115
TAGAGTG4100.014.4390245
AGGTTAT4400.014.2954541
TTAGAGT4150.014.265064
CTATACT3250.014.230774
TTATCTA4850.014.1134024
ATTATAC4350.014.0344833
TTATACC3050.013.950824
AACGTCT3200.013.8750016
CACTCTA5500.013.7909099
GAGTGTT3650.013.6849327
Produced by FastQC (version 0.11.5)