##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727111.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2844637
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.42642101610856	31.0	30.0	31.0	28.0	34.0
2	30.820590817035708	31.0	31.0	34.0	27.0	34.0
3	31.149020068289907	31.0	31.0	34.0	28.0	34.0
4	35.05450080273863	35.0	35.0	37.0	32.0	37.0
5	34.75874109772178	35.0	35.0	37.0	32.0	37.0
6	34.665880391768795	35.0	35.0	37.0	31.0	37.0
7	34.50754560247933	35.0	35.0	37.0	30.0	37.0
8	34.51517293770699	35.0	35.0	37.0	30.0	37.0
9	35.847347130758685	37.0	35.0	39.0	30.0	39.0
10	35.70412745105966	37.0	35.0	39.0	30.0	39.0
11	35.691514593953464	37.0	35.0	39.0	30.0	39.0
12	35.52441594481124	37.0	35.0	39.0	30.0	39.0
13	35.63913919421002	37.0	35.0	39.0	30.0	39.0
14	36.36600100469761	38.0	35.0	40.0	30.0	41.0
15	36.380523420035665	38.0	35.0	40.0	30.0	41.0
16	36.20912474948473	38.0	34.0	40.0	30.0	41.0
17	36.22509831658662	38.0	34.0	40.0	30.0	41.0
18	36.10346135552621	38.0	34.0	40.0	29.0	41.0
19	36.04901328359295	38.0	34.0	40.0	29.0	41.0
20	36.5170754651648	38.0	35.0	40.0	30.0	41.0
21	36.613449800449054	38.0	35.0	40.0	30.0	41.0
22	36.60637086559726	38.0	35.0	40.0	30.0	41.0
23	36.52675402872141	38.0	35.0	40.0	30.0	41.0
24	36.39391458382915	38.0	35.0	40.0	30.0	41.0
25	36.08752962152992	38.0	34.0	40.0	29.0	41.0
26	35.8697946346054	38.0	34.0	40.0	27.0	41.0
27	35.69570071682257	38.0	34.0	40.0	27.0	41.0
28	35.442677220327234	38.0	34.0	40.0	27.0	41.0
29	35.27222489196337	38.0	34.0	40.0	26.0	41.0
30	34.93031096762083	38.0	33.0	40.0	25.0	41.0
31	34.765154218271086	38.0	33.0	40.0	25.0	41.0
32	34.562856350388465	37.0	33.0	40.0	25.0	41.0
33	34.49909355745566	37.0	33.0	40.0	24.0	41.0
34	34.301855034579106	37.0	33.0	40.0	24.0	41.0
35	33.906623938309174	37.0	32.0	40.0	23.0	41.0
36	33.886940934818746	37.0	32.0	40.0	23.0	41.0
37	33.72811856134895	37.0	32.0	40.0	22.0	41.0
38	33.598970624371404	37.0	32.0	40.0	21.0	41.0
39	33.45465273776584	37.0	31.0	40.0	20.0	41.0
40	33.204668996430826	36.0	31.0	40.0	19.0	41.0
41	32.97138931962145	36.0	31.0	40.0	18.0	41.0
42	32.750292568085136	36.0	31.0	40.0	15.0	41.0
43	32.545977219589005	36.0	30.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	6.0
11	17.0
12	26.0
13	26.0
14	54.0
15	116.0
16	202.0
17	459.0
18	828.0
19	1692.0
20	3382.0
21	6075.0
22	10304.0
23	16167.0
24	23227.0
25	32937.0
26	44452.0
27	58755.0
28	74715.0
29	94124.0
30	114812.0
31	139278.0
32	166728.0
33	198242.0
34	231943.0
35	268968.0
36	316857.0
37	367100.0
38	407384.0
39	265756.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.58020337920093	23.38590125910617	15.064206786314035	24.96968857537886
2	18.269466367765023	26.10371727570161	31.127697488291123	24.49911886824224
3	20.11325170839021	26.716625003471446	30.3949853707169	22.77513791742145
4	16.183822399835197	18.04121228824627	31.404499062622047	34.370466249296484
5	13.254134007256463	40.91991350741765	31.95550082488556	13.870451660440331
6	33.532222213238455	36.913778453982005	14.603796547679018	14.950202785100524
7	27.132354673021545	33.31940068275846	20.62305313472334	18.925191509496642
8	28.5490556440066	35.74238118958588	19.223436944678706	16.48512622172882
9	28.460538198722716	14.453619213980554	18.988538783683122	38.097303803613606
10	17.720011375792414	29.962698228280093	31.179338523685097	21.1379518722424
11	34.960277884313534	23.824305174966085	19.49932451838319	21.71609242233719
12	20.976068299751425	29.66287086893688	28.618589999356686	20.74247083195501
13	30.377830282035983	20.765074770524322	25.55767220914303	23.29942273829666
14	22.688905473703674	22.471373324610486	25.839465633049137	29.000255568636703
15	26.22134212555064	28.530775631477763	22.038383104768727	23.209499138202872
16	22.875642832459818	27.172887085417223	26.164744394451734	23.786725687671222
17	23.23449353994903	29.131906812714593	25.531517729678686	22.102081917657685
18	23.567260075714405	25.55826982493724	27.55585334789641	23.31861675145194
19	24.36901439445525	27.57698082391532	26.78686243622648	21.267142345402945
20	23.568666230524315	26.131066986754377	27.76431579846567	22.53595098425564
21	23.677080766368434	27.734083470052596	26.922099375069646	21.666736388509324
22	23.8091538568893	27.40729309222934	25.327133128058165	23.456419922823194
23	22.319192220307897	27.29058224300675	26.556463970622612	23.833761566062737
24	22.319860143842607	29.142312358307933	26.076367564648844	22.46145993320062
25	23.264093098697654	27.69000051676189	25.30909919262106	23.73680719191939
26	23.12653600441814	27.702691063921336	26.459122903906547	22.71165002775398
27	23.689454928695646	27.939557841650796	25.398847023363615	22.972140206289943
28	22.53148644273417	28.236994737817163	26.84275708992044	22.38876172952823
29	22.908617162752222	27.69379713474865	27.023131598161733	22.374454104337392
30	22.546075298886993	28.91426920201066	27.018245210197296	21.521410288905052
31	23.41093081472258	27.920785674938493	25.700221152997727	22.9680623573412
32	21.168711508709194	28.94091583565847	26.451599975673524	23.438772679958813
33	21.907434938095793	28.166124535397664	27.06492954988633	22.861510976620217
34	21.76358530104193	28.35328374059678	27.04148191843107	22.841649039930225
35	22.186943360435794	28.882595564917423	27.094458800894454	21.836002273752328
36	22.57402262573397	28.43550864310631	26.249746452710838	22.740722278448885
37	21.868203218899282	28.560199420875144	26.890003891533436	22.68159346869214
38	22.355154629571366	27.900044891492303	27.25110444671851	22.49369603221782
39	22.313251216236026	26.93345407515968	27.283516315086953	23.469778393517345
40	22.144020484863272	27.770467725759033	27.636250249153054	22.44926154022464
41	21.1914560627595	27.935620608183047	27.75893725631777	23.113986072739685
42	22.017466551971307	28.33939796184891	27.677345123472698	21.96579036270709
43	21.713491035938855	26.977783105542112	27.914668901515377	23.394056957003652
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	777.0
1	1154.5
2	1532.0
3	1576.5
4	1621.0
5	1621.0
6	2164.0
7	2707.0
8	4107.5
9	5508.0
10	9136.5
11	12765.0
12	12765.0
13	26922.0
14	41079.0
15	51039.5
16	61000.0
17	47643.5
18	34287.0
19	34287.0
20	38575.5
21	42864.0
22	38867.0
23	34870.0
24	37590.5
25	40311.0
26	40311.0
27	44634.0
28	48957.0
29	52375.5
30	55794.0
31	60485.0
32	65176.0
33	65176.0
34	74580.5
35	83985.0
36	95747.5
37	107510.0
38	120162.0
39	132814.0
40	132814.0
41	142918.0
42	153022.0
43	165051.0
44	177080.0
45	198345.0
46	219610.0
47	219610.0
48	264772.5
49	309935.0
50	301095.5
51	292256.0
52	247628.5
53	203001.0
54	203001.0
55	186679.5
56	170358.0
57	159858.0
58	149358.0
59	137145.5
60	124933.0
61	124933.0
62	106602.0
63	88271.0
64	75749.0
65	63227.0
66	52931.5
67	42636.0
68	42636.0
69	34712.0
70	26788.0
71	23524.5
72	20261.0
73	16115.0
74	11969.0
75	11969.0
76	9244.5
77	6520.0
78	5518.5
79	4517.0
80	3573.0
81	2629.0
82	2629.0
83	2182.0
84	1735.0
85	1366.0
86	997.0
87	1006.5
88	1016.0
89	1016.0
90	734.5
91	453.0
92	352.5
93	252.0
94	214.5
95	177.0
96	177.0
97	108.0
98	39.0
99	39.5
100	40.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2844637.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.14746999322222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.75270340736617	47.29044637995411
2	7.470987433961074	8.240121106682173
3	2.377170335553255	3.932847892461034
4	1.2296208325950928	2.712419118743152
5	0.720697936914424	1.9872333925082684
6	0.4679992977204441	1.5485386336732359
7	0.33387409101964793	1.2888617991214266
8	0.24701007668012256	1.0897584633392463
9	0.18908667958651582	0.9384886789753865
>10	1.0636131846017627	10.610175453315799
>50	0.0758184766803623	2.9088305110141164
>100	0.05977497452959189	6.89592104310916
>500	0.006525350685449659	2.51716150488305
>1k	0.004478181842941639	4.302791028428589
>5k	4.4781818429416386E-4	1.7360610435050057
>10k+	1.919220789832131E-4	2.000343950286342
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27079	0.9519316524393094	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17944	0.6308010477259489	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11676	0.410456589012939	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	9832	0.3456328522760549	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	9033	0.31754490994808826	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	6837	0.24034701088398977	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6749	0.2372534703021862	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	6118	0.2150713781758446	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	5559	0.19542036470734228	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	5080	0.1785816608586614	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4596	0.1615671876587417	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	4199	0.1476111011703778	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	3846	0.1352017849729157	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3729	0.13108878215392683	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	3533	0.1241986235853643	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	3488	0.12261669942421474	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3395	0.11934738949117232	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	3332	0.11713269566556296	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	2900	0.10194622371852717	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.515387024776799E-5
2	0.0	0.0	0.0	0.0	3.515387024776799E-5
3	0.0	0.0	0.0	3.515387024776799E-5	3.515387024776799E-5
4	0.0	0.0	0.0	3.515387024776799E-5	3.515387024776799E-5
5	0.0	0.0	0.0	3.515387024776799E-5	3.515387024776799E-5
6	0.0	0.0	0.0	3.515387024776799E-5	7.030774049553599E-5
7	0.0	0.0	0.0	3.515387024776799E-5	7.030774049553599E-5
8	0.0	0.0	0.0	3.515387024776799E-5	7.030774049553599E-5
9	0.0	0.0	0.0	7.030774049553599E-5	7.030774049553599E-5
10	0.0	0.0	0.0	1.7576935123883995E-4	7.030774049553599E-5
11	0.0	0.0	0.0	3.515387024776799E-4	7.030774049553599E-5
12	0.0	0.0	0.0	4.570003132209839E-4	7.030774049553599E-5
13	0.0	0.0	0.0	5.624619239642879E-4	7.030774049553599E-5
14	3.515387024776799E-5	0.0	0.0	9.491544966897358E-4	1.0546161074330398E-4
15	3.515387024776799E-5	0.0	0.0	0.0013006931991674158	1.0546161074330398E-4
16	7.030774049553599E-5	0.0	0.0	0.0024256170470959913	1.4061548099107197E-4
17	7.030774049553599E-5	0.0	0.0	0.004464541521466535	1.4061548099107197E-4
18	7.030774049553599E-5	0.0	0.0	0.005624619239642878	1.4061548099107197E-4
19	7.030774049553599E-5	0.0	0.0	0.007944774675995567	1.4061548099107197E-4
20	7.030774049553599E-5	0.0	0.0	0.010405545593339326	1.4061548099107197E-4
21	7.030774049553599E-5	0.0	0.0	0.01662778062719426	1.4061548099107197E-4
22	7.030774049553599E-5	0.0	0.0	0.02576778689161394	1.4061548099107197E-4
23	7.030774049553599E-5	0.0	0.0	0.04309864492376356	1.4061548099107197E-4
24	7.030774049553599E-5	0.0	0.0	0.0800805164244155	1.4061548099107197E-4
25	7.030774049553599E-5	0.0	0.0	0.10405545593339326	1.4061548099107197E-4
26	7.030774049553599E-5	0.0	0.0	0.1305966279704581	1.4061548099107197E-4
27	7.030774049553599E-5	0.0	0.0	0.16982834716696718	1.4061548099107197E-4
28	7.030774049553599E-5	0.0	0.0	0.24287808954182907	1.4061548099107197E-4
29	7.030774049553599E-5	0.0	0.0	0.31525990838198337	1.7576935123883995E-4
30	7.030774049553599E-5	0.0	0.0	0.4159405927715909	1.7576935123883995E-4
31	7.030774049553599E-5	0.0	0.0	0.6331563570325494	1.7576935123883995E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	170	0.0	25.029413	4
TCGAACG	185	0.0	23.0	3
ATTCGAA	225	0.0	18.911112	1
TACCGTA	50	0.0070373695	18.5	7
AAGACGG	710	0.0	15.633802	5
CAAGACG	695	0.0	15.4388485	4
GAACGTC	280	0.0	15.196428	5
ACCGTAT	75	0.0041063856	14.8	8
TATCTAG	575	0.0	14.478261	1
GTGCGTA	230	1.8189894E-12	14.478261	15
TAGAGTG	410	0.0	14.439024	5
AGGTTAT	440	0.0	14.295454	1
TTAGAGT	415	0.0	14.26506	4
CTATACT	325	0.0	14.23077	4
TTATCTA	485	0.0	14.113402	4
ATTATAC	435	0.0	14.034483	3
TTATACC	305	0.0	13.95082	4
AACGTCT	320	0.0	13.875001	6
CACTCTA	550	0.0	13.790909	9
GAGTGTT	365	0.0	13.684932	7
>>END_MODULE