FastQCFastQC Report
Thu 9 Feb 2017
SRR2727110.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727110.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4214146
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT340410.8077793223110922No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216020.5126068247279519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT146690.34808950615379725No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG124510.29545725278621104No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC118870.2820737582418834No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA84210.19982696375493397No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT83450.19802351413548558No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG75740.17972799233818668No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT71310.16921577942482297No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG61950.14700487358530057No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG57800.13715708947910205No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55540.13179419982126866No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC48940.11613266365237465No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45370.10766119636101835No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG44400.10535942513619605No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC43670.10362716431751534No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA42210.10016264268015394No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42170.10006772427913033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT3600.018.54
TCGAACG3650.017.7397253
AGTCGCG752.0689146E-417.26666634
ACGAACG4400.016.39772815
AAGACGG11500.016.2478265
CGAACGA4400.015.97727316
CGATAAC4350.015.31034410
TATCTAG10850.015.1751161
GTATTAG10300.014.9077671
AGGTTAT6150.014.7398381
CAAGACG12750.014.6549024
CCGATAA4550.014.63736259
GGTTATC6350.014.5669292
TAGAGTG6150.014.4390245
ATTCGAA4750.014.4105261
CGCTTCG7600.014.36184232
CACTCTA8400.014.3154759
TAGAGTC12450.014.265065
CTAGAGT13500.014.2518524
AACGAAC5000.014.05999914
Produced by FastQC (version 0.11.5)