##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727110.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4214146
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.420292510036436	31.0	30.0	31.0	27.0	34.0
2	30.813290521970526	31.0	31.0	33.0	27.0	34.0
3	31.122345547591376	31.0	31.0	34.0	28.0	34.0
4	35.02920330714693	35.0	35.0	37.0	32.0	37.0
5	34.73349665626203	35.0	35.0	37.0	32.0	37.0
6	34.630388695598114	35.0	35.0	37.0	31.0	37.0
7	34.475875064603834	35.0	35.0	37.0	30.0	37.0
8	34.475110496883595	35.0	35.0	37.0	30.0	37.0
9	35.819430081444736	37.0	35.0	39.0	30.0	39.0
10	35.60351634708432	37.0	35.0	39.0	30.0	39.0
11	35.64371381532581	37.0	35.0	39.0	30.0	39.0
12	35.24518348438806	37.0	34.0	39.0	29.0	39.0
13	35.51998625581553	37.0	35.0	39.0	30.0	39.0
14	36.212287376849304	38.0	34.0	40.0	30.0	41.0
15	36.24471553667101	38.0	34.0	40.0	30.0	41.0
16	36.09596748665091	38.0	34.0	40.0	29.0	41.0
17	36.04078192829579	38.0	34.0	40.0	29.0	41.0
18	35.98174054719509	38.0	34.0	40.0	29.0	41.0
19	35.966025382129615	38.0	34.0	40.0	28.0	41.0
20	36.40155514308237	38.0	35.0	40.0	30.0	41.0
21	36.509870327226444	38.0	35.0	40.0	30.0	41.0
22	36.49076325310039	38.0	35.0	40.0	30.0	41.0
23	36.38776516048566	38.0	35.0	40.0	30.0	41.0
24	36.26697485089505	38.0	35.0	40.0	29.0	41.0
25	35.98138436589525	38.0	34.0	40.0	28.0	41.0
26	35.75472088532291	38.0	34.0	40.0	27.0	41.0
27	35.59528502334755	38.0	34.0	40.0	27.0	41.0
28	35.3491378798931	38.0	34.0	40.0	27.0	41.0
29	35.17603329357834	38.0	34.0	40.0	26.0	41.0
30	34.86146042400999	38.0	33.0	40.0	25.0	41.0
31	34.704661869807076	38.0	33.0	40.0	25.0	41.0
32	34.50276805786985	37.0	33.0	40.0	24.0	41.0
33	34.451428593124206	37.0	33.0	40.0	24.0	41.0
34	34.20572804074657	37.0	33.0	40.0	24.0	41.0
35	33.84372539537073	37.0	32.0	40.0	23.0	41.0
36	33.744527835532985	37.0	32.0	40.0	22.0	41.0
37	33.538938612947916	37.0	31.0	40.0	21.0	41.0
38	33.48251057272339	37.0	31.0	40.0	21.0	41.0
39	33.294287620789596	37.0	31.0	40.0	19.0	41.0
40	33.04778002470726	36.0	31.0	40.0	18.0	41.0
41	32.87418613403522	36.0	31.0	40.0	17.0	41.0
42	32.67343585153433	36.0	30.0	39.0	15.0	41.0
43	32.527652340474205	36.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	14.0
11	31.0
12	41.0
13	36.0
14	87.0
15	195.0
16	421.0
17	739.0
18	1361.0
19	2892.0
20	5464.0
21	9834.0
22	15925.0
23	25065.0
24	36416.0
25	51191.0
26	68347.0
27	90277.0
28	114804.0
29	143398.0
30	175820.0
31	212737.0
32	250907.0
33	296599.0
34	346316.0
35	399411.0
36	464660.0
37	529130.0
38	589267.0
39	382759.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.32460764292457	23.344634950948542	15.070028423315188	25.260728982811703
2	18.436238326816394	25.69220430426473	31.471999308994043	24.399558059924832
3	19.77686582287372	26.574636948980885	30.430863097766427	23.21763413037897
4	15.925148298136799	17.87197216233135	32.093809754099645	34.10906978543221
5	13.207373451228316	40.754947740301354	32.32697680621412	13.71070200225621
6	33.53979667529317	37.436980114120395	14.570449149127723	14.452774061458715
7	27.40486447313406	33.10694503702529	20.784590756941025	18.70359973289962
8	28.372201627565826	35.838032189677335	19.107904662059642	16.681861520697193
9	28.24251936216733	14.47591042170822	18.881761571620917	38.39980864450354
10	17.46270774671784	29.67963141286514	31.46355157130294	21.394109269114075
11	35.47867112340199	23.57981901908477	19.66794695769914	21.273562899814102
12	21.087926237012198	29.153190231188002	28.693239389427895	21.065644142371905
13	30.29928246434746	20.57550924908629	25.358138991862173	23.767069294704076
14	22.66857863965795	22.325757104760964	25.516723910372352	29.488940345208732
15	26.05220606974699	28.655438136220248	21.79672939665593	23.495626397376835
16	23.360201568716413	27.043059258032354	25.54016875542518	24.056570417826055
17	23.435187105525056	28.76428581259406	25.284719608670414	22.515807473210465
18	23.87342536305102	25.429849843835502	27.08831160572035	23.60841318739313
19	24.63312851524366	27.15805290087244	26.3776338076564	21.831184776227495
20	23.885622377582553	25.824496825691373	27.103332442682337	23.186548354043737
21	24.098049759073366	27.33089930913642	26.354995769012273	22.21605516277794
22	24.017274199802287	26.94526957537779	25.17067514984056	23.866781074979368
23	22.667843022050018	26.783362512831783	26.19757360091463	24.351220864203565
24	22.819285330883172	28.427610244163347	25.805299579084352	22.947804845869126
25	23.62519001477405	27.13890311346593	25.09417091861554	24.14173595314448
26	23.575974823843314	27.268419271662637	26.074867837991373	23.08073806650268
27	24.055644963416075	27.37968736726255	25.152972868049662	23.411694801271718
28	22.99455215837325	27.567886826892092	26.4928410168988	22.94471999783586
29	23.308684606560856	27.26101563638279	26.606102398920207	22.824197358136146
30	23.027821057932023	28.22780701000867	26.5893730307398	22.15499890131951
31	23.848105879577975	27.347201544512224	25.45149123926888	23.35320133664092
32	21.757148423429086	28.254502810296557	26.081583314863792	23.906765451410557
33	22.498128921019823	27.461198544141563	26.680637073323993	23.360035461514624
34	22.491674469750215	27.628444766745147	26.690840801434028	23.189039962070606
35	22.924217623214762	28.147126369138608	26.633913490420124	22.294742517226503
36	23.104254100356275	27.768710433857773	26.05953851622606	23.06749694955989
37	22.5943524500575	27.733139763074178	26.59786822763141	23.07463955923691
38	22.850560944020447	27.11344125239135	27.001437539183502	23.034560264404696
39	22.929105920867478	26.326567707905706	26.909224312589075	23.83510205863774
40	22.80910533237339	27.07734853040213	27.347818514118877	22.765727623105604
41	21.759473924254166	27.299030455992746	27.50690175423443	23.43459386551866
42	22.76138510625878	27.569619087710773	27.45645262409038	22.212543181940067
43	22.30262074451146	26.337649431225213	27.57042589411947	23.789303930143856
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	969.0
1	1427.5
2	1886.0
3	1911.0
4	1936.0
5	1936.0
6	2520.0
7	3104.0
8	4877.5
9	6651.0
10	11210.5
11	15770.0
12	15770.0
13	32722.0
14	49674.0
15	62615.0
16	75556.0
17	59414.0
18	43272.0
19	43272.0
20	49383.5
21	55495.0
22	50561.5
23	45628.0
24	50441.5
25	55255.0
26	55255.0
27	62524.5
28	69794.0
29	75887.5
30	81981.0
31	89343.5
32	96706.0
33	96706.0
34	111935.5
35	127165.0
36	145971.0
37	164777.0
38	184945.0
39	205113.0
40	205113.0
41	219117.0
42	233121.0
43	248757.5
44	264394.0
45	294601.5
46	324809.0
47	324809.0
48	384183.0
49	443557.0
50	430801.0
51	418045.0
52	361632.5
53	305220.0
54	305220.0
55	283322.0
56	261424.0
57	247578.5
58	233733.0
59	215262.5
60	196792.0
61	196792.0
62	167537.5
63	138283.0
64	118527.0
65	98771.0
66	83462.5
67	68154.0
68	68154.0
69	55780.5
70	43407.0
71	38463.5
72	33520.0
73	26508.0
74	19496.0
75	19496.0
76	15091.5
77	10687.0
78	9107.5
79	7528.0
80	5927.0
81	4326.0
82	4326.0
83	3651.0
84	2976.0
85	2415.5
86	1855.0
87	1810.5
88	1766.0
89	1766.0
90	1218.0
91	670.0
92	552.0
93	434.0
94	370.5
95	307.0
96	307.0
97	183.0
98	59.0
99	69.5
100	80.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4214146.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.98592919948608
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.46354233535045	43.909157317833234
2	7.982279190689627	8.299324017154442
3	2.692849663321577	4.199708758268863
4	1.3065151074289605	2.716816074914435
5	0.7762122962114795	2.017605873731025
6	0.5102602289472135	1.591581128121804
7	0.35441537633771536	1.289722886305118
8	0.2737947391765695	1.138677914081992
9	0.21112824379932832	0.9878128140747346
>10	1.2587517761721063	12.033350184420497
>50	0.09213222765950374	3.3066358875712965
>100	0.06310862984325484	6.61563963786996
>500	0.008656180404252124	3.0913774328257317
>1k	0.005801482514067889	5.3641624761476026
>5k	3.223045841148823E-4	1.1728712303326256
>10k+	2.3021756008205884E-4	2.2655563663465923
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34041	0.8077793223110922	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21602	0.5126068247279519	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14669	0.34808950615379725	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	12451	0.29545725278621104	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	11887	0.2820737582418834	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	8421	0.19982696375493397	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8345	0.19802351413548558	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	7574	0.17972799233818668	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	7131	0.16921577942482297	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	6195	0.14700487358530057	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	5780	0.13715708947910205	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5554	0.13179419982126866	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	4894	0.11613266365237465	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4537	0.10766119636101835	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	4440	0.10535942513619605	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	4367	0.10362716431751534	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	4221	0.10016264268015394	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4217	0.10006772427913033	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.3729600255900008E-5	0.0
5	0.0	0.0	0.0	2.3729600255900008E-5	0.0
6	2.3729600255900008E-5	0.0	0.0	2.3729600255900008E-5	4.7459200511800015E-5
7	2.3729600255900008E-5	0.0	0.0	2.3729600255900008E-5	4.7459200511800015E-5
8	2.3729600255900008E-5	0.0	0.0	4.7459200511800015E-5	4.7459200511800015E-5
9	2.3729600255900008E-5	0.0	0.0	9.491840102360003E-5	7.118880076770003E-5
10	4.7459200511800015E-5	0.0	0.0	1.1864800127950004E-4	7.118880076770003E-5
11	4.7459200511800015E-5	0.0	0.0	1.6610720179130006E-4	7.118880076770003E-5
12	4.7459200511800015E-5	0.0	0.0	2.135664023031001E-4	7.118880076770003E-5
13	4.7459200511800015E-5	0.0	0.0	2.135664023031001E-4	7.118880076770003E-5
14	4.7459200511800015E-5	0.0	0.0	5.220512056298002E-4	9.491840102360003E-5
15	4.7459200511800015E-5	0.0	0.0	8.542656092124004E-4	9.491840102360003E-5
16	4.7459200511800015E-5	0.0	0.0	0.0019695568212397007	1.1864800127950004E-4
17	4.7459200511800015E-5	0.0	0.0	0.003488251237617301	1.1864800127950004E-4
18	4.7459200511800015E-5	0.0	0.0	0.0042238688455502015	1.1864800127950004E-4
19	4.7459200511800015E-5	0.0	0.0	0.006051048065254502	1.1864800127950004E-4
20	4.7459200511800015E-5	0.0	0.0	0.007664660882655703	1.1864800127950004E-4
21	4.7459200511800015E-5	0.0	0.0	0.011437667323343805	1.4237760153540006E-4
22	4.7459200511800015E-5	0.0	0.0	0.018200603396275307	1.4237760153540006E-4
23	4.7459200511800015E-5	0.0	0.0	0.031346801938043914	2.135664023031001E-4
24	4.7459200511800015E-5	0.0	0.0	0.05619169340597122	2.135664023031001E-4
25	4.7459200511800015E-5	0.0	0.0	0.07218544397844782	2.135664023031001E-4
26	4.7459200511800015E-5	0.0	0.0	0.09059961377702623	2.135664023031001E-4
27	4.7459200511800015E-5	0.0	0.0	0.11914632288487395	2.135664023031001E-4
28	4.7459200511800015E-5	0.0	0.0	0.17346337787062907	2.135664023031001E-4
29	4.7459200511800015E-5	0.0	0.0	0.23131614329451328	2.135664023031001E-4
30	4.7459200511800015E-5	0.0	0.0	0.3158409794060291	2.135664023031001E-4
31	4.7459200511800015E-5	0.0	0.0	0.5122983399246253	2.135664023031001E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	360	0.0	18.5	4
TCGAACG	365	0.0	17.739725	3
AGTCGCG	75	2.0689146E-4	17.266666	34
ACGAACG	440	0.0	16.397728	15
AAGACGG	1150	0.0	16.247826	5
CGAACGA	440	0.0	15.977273	16
CGATAAC	435	0.0	15.310344	10
TATCTAG	1085	0.0	15.175116	1
GTATTAG	1030	0.0	14.907767	1
AGGTTAT	615	0.0	14.739838	1
CAAGACG	1275	0.0	14.654902	4
CCGATAA	455	0.0	14.6373625	9
GGTTATC	635	0.0	14.566929	2
TAGAGTG	615	0.0	14.439024	5
ATTCGAA	475	0.0	14.410526	1
CGCTTCG	760	0.0	14.361842	32
CACTCTA	840	0.0	14.315475	9
TAGAGTC	1245	0.0	14.26506	5
CTAGAGT	1350	0.0	14.251852	4
AACGAAC	500	0.0	14.059999	14
>>END_MODULE