FastQCFastQC Report
Thu 9 Feb 2017
SRR2727109.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727109.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349570
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT134730.9983179827648807No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93260.6910349222344895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49840.36930281497069434No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG49120.3639677823306683No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC40230.2980949487614573No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA39880.2955015301170002No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG38880.28809176256140845No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT34470.2554146876412487No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG31860.23607519432115415No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG25690.19035692850315283No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23110.17123972820972605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21920.16242210481857183No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19580.14508324873848708No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC19550.1448609557118193No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC17580.13026371362730352No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC16580.12285394607171173No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA16410.12159428558726113No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16060.11900086694280401No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15630.11581466689389955No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG15350.11373993197833383No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC14760.10936816912053468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAG2000.018.53
GTAGGAC1103.8531653E-818.53
TCGGTAA500.007035500418.522
TAGGACT1552.1827873E-1117.9032274
GTATTAG2550.017.4117641
TAGAGTG1950.017.0769215
CTAGGAC2100.016.7380943
TTAGAGT1901.8189894E-1216.5526314
CGATGCG1951.8189894E-1216.12820433
TATTAGC3400.015.7794132
TTTAGAC1201.9360104E-615.4166663
GGAGTGT3250.015.3692316
TAAGACA2055.456968E-1215.3414644
GTACCGT855.365008E-415.2352956
CTATTCA855.365008E-415.2352954
TTAGCTC3050.015.1639344
TGCGGCG2201.8189894E-1215.13636436
TACACCG1353.9733277E-715.0740735
ATTAGCT3200.015.031253
TACTTAC2350.014.95744731
Produced by FastQC (version 0.11.5)