##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727109.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1349570
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.970048978563543	31.0	30.0	31.0	27.0	34.0
2	30.417893106693242	31.0	30.0	31.0	27.0	34.0
3	30.722747986395667	31.0	30.0	34.0	27.0	34.0
4	34.649603947924156	35.0	35.0	37.0	32.0	37.0
5	34.315199656186785	35.0	35.0	37.0	30.0	37.0
6	34.26648784427633	35.0	35.0	37.0	30.0	37.0
7	34.11469356906274	35.0	35.0	37.0	30.0	37.0
8	34.11078565765392	35.0	33.0	37.0	30.0	37.0
9	35.39537185918478	37.0	34.0	39.0	30.0	39.0
10	35.216038441874076	37.0	34.0	39.0	29.0	39.0
11	35.25640463258667	37.0	34.0	39.0	30.0	39.0
12	35.05469816311862	37.0	34.0	39.0	29.0	39.0
13	35.21707729128537	37.0	34.0	39.0	30.0	39.0
14	35.83975933074979	38.0	34.0	40.0	28.0	41.0
15	35.905849270508384	38.0	34.0	40.0	29.0	41.0
16	35.701305601043295	38.0	34.0	40.0	27.0	41.0
17	35.732952718273225	38.0	34.0	40.0	27.0	41.0
18	35.61611105759612	38.0	33.0	40.0	27.0	41.0
19	35.597745207732835	38.0	34.0	40.0	27.0	41.0
20	36.01243877679557	38.0	34.0	40.0	29.0	41.0
21	36.089017983505855	38.0	34.0	40.0	29.0	41.0
22	36.07530694962099	38.0	34.0	40.0	29.0	41.0
23	35.95832450336033	38.0	34.0	40.0	28.0	41.0
24	35.82402468934549	38.0	34.0	40.0	27.0	41.0
25	35.526346169520664	38.0	34.0	40.0	27.0	41.0
26	35.27568632971984	38.0	34.0	40.0	27.0	41.0
27	35.08808212986359	38.0	33.0	40.0	26.0	41.0
28	34.824987959127725	38.0	33.0	40.0	25.0	41.0
29	34.65486340093511	37.0	33.0	40.0	25.0	41.0
30	34.29938276636262	37.0	33.0	40.0	24.0	41.0
31	34.118848225731156	37.0	32.0	40.0	24.0	41.0
32	33.920447994546414	37.0	32.0	40.0	24.0	41.0
33	33.8577813673985	37.0	32.0	40.0	24.0	41.0
34	33.64726913016739	36.0	31.0	39.0	23.0	40.0
35	33.232681520780694	36.0	31.0	39.0	20.0	40.0
36	33.232848240550695	36.0	31.0	39.0	20.0	40.0
37	33.049132686707615	36.0	31.0	39.0	19.0	40.0
38	32.94260023563061	36.0	31.0	39.0	19.0	40.0
39	32.77901109242203	36.0	30.0	39.0	18.0	40.0
40	32.53497262090888	35.0	30.0	39.0	16.0	40.0
41	32.28370666212201	35.0	30.0	39.0	15.0	40.0
42	32.04616062894107	35.0	30.0	39.0	15.0	40.0
43	31.83874863845521	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	7.0
12	8.0
13	16.0
14	40.0
15	76.0
16	157.0
17	312.0
18	646.0
19	1219.0
20	2347.0
21	4093.0
22	6548.0
23	9932.0
24	14245.0
25	19666.0
26	25911.0
27	33827.0
28	42510.0
29	51971.0
30	62179.0
31	73623.0
32	86313.0
33	100902.0
34	115399.0
35	131750.0
36	151173.0
37	168285.0
38	171713.0
39	74698.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.29670191246101	25.881577094926534	16.2630319286884	23.558689063924064
2	19.542520951117766	27.924227716976517	28.926843364923645	23.606407966982076
3	21.67594122572375	26.896122468638158	30.379083708144076	21.048852597494015
4	18.144964692457595	19.15069244277807	30.442807709122167	32.26153515564217
5	13.72703898278711	41.24684158657943	31.523966893158562	13.502152537474899
6	33.6705765540135	37.57930303726372	13.923620116036961	14.826500292685818
7	26.294671636150774	33.76512518802286	20.80499714723949	19.135206028586882
8	28.232251754262467	36.30912068288418	19.47901924316634	15.97960831968701
9	28.149706943693175	14.42459450047052	19.135057833235773	38.290640722600536
10	17.644731284779596	30.43895462999326	31.58880235926999	20.327511725957155
11	35.069614766184785	23.695621568351402	19.39410330697926	21.840660358484556
12	20.342257163392784	30.44177034166438	28.874308112954495	20.341664381988338
13	30.553880124780484	20.88939439969768	25.872537178508708	22.684188297013122
14	22.520802922412326	22.42121564646517	26.374178442022274	28.683802989100233
15	26.183376927465783	28.956704728172678	22.04731877561001	22.81259956875153
16	22.557555369488057	27.520617678223434	26.32379202264425	23.598034929644257
17	22.835347555147194	29.989404032395505	25.85979237831309	21.31545603414421
18	23.127440592188623	25.768800432730426	28.04270990019043	23.061049074890523
19	24.238016553420717	28.32946790459183	26.645227739205822	20.787287802781627
20	23.436798387634582	26.299339789710796	28.04848951888379	22.21537230377083
21	23.28741747371385	28.463362404321373	26.853738598220172	21.3954815237446
22	23.539942352008417	28.228324577458004	25.459516734960026	22.772216335573553
23	22.147276539934943	27.600643167823826	26.87648658461584	23.37559370762539
24	22.14186740961936	29.665374897189473	26.343872492719907	21.848885200471262
25	23.037856502441517	28.24655260564476	25.58607556480953	23.129515327104187
26	22.75628533532903	28.351623109583052	26.75289166178857	22.139199893299345
27	23.625302874248835	28.429351571241213	25.624161770045273	22.321183784464683
28	22.270352779033324	28.667279207451262	27.331816800906957	21.73055121260846
29	22.69589572975096	28.069533258741675	27.192587268537388	22.041983742969983
30	22.244714983290976	29.323784612876697	27.35789918270264	21.073601221129692
31	23.11862296879747	28.613558392673223	25.830968382521842	22.436850256007467
32	20.737790555510273	29.650703557429402	26.567647472898777	23.043858414161548
33	21.85673955408019	28.594367094704236	27.35330512681817	22.1955882243974
34	21.43504968249146	28.91780344850582	27.121083011625924	22.526063857376794
35	21.77841831101758	29.619360240669252	27.341301303378113	21.260920144935053
36	22.53888275524797	29.06955548804434	26.146402187363382	22.24515956934431
37	21.46646709692717	29.2155279088895	27.063435020043418	22.254569974139912
38	22.22952495980201	28.41631038034337	27.43592403506302	21.9182406247916
39	22.13097505131264	27.391391332053917	27.29750957712457	23.180124039508883
40	21.932467378498334	28.42572078513897	27.681631927206446	21.96017990915625
41	21.08530865386753	28.430314841023435	27.689412183139815	22.794964321969218
42	21.87815378231585	29.036656120097515	27.672740206139736	21.412449891446904
43	21.66556755114592	27.56626184636588	27.95438547092778	22.813785131560422
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	465.0
1	625.5
2	786.0
3	837.5
4	889.0
5	889.0
6	1093.5
7	1298.0
8	2078.0
9	2858.0
10	4600.0
11	6342.0
12	6342.0
13	13815.0
14	21288.0
15	26605.0
16	31922.0
17	24852.0
18	17782.0
19	17782.0
20	19892.5
21	22003.0
22	20292.5
23	18582.0
24	19225.5
25	19869.0
26	19869.0
27	22047.0
28	24225.0
29	25924.5
30	27624.0
31	29797.5
32	31971.0
33	31971.0
34	36647.0
35	41323.0
36	46765.5
37	52208.0
38	57930.0
39	63652.0
40	63652.0
41	68530.5
42	73409.0
43	78714.0
44	84019.0
45	95585.5
46	107152.0
47	107152.0
48	131284.5
49	155417.0
50	148638.5
51	141860.0
52	117969.0
53	94078.0
54	94078.0
55	84726.5
56	75375.0
57	70370.5
58	65366.0
59	59273.5
60	53181.0
61	53181.0
62	45245.0
63	37309.0
64	31821.5
65	26334.0
66	22144.0
67	17954.0
68	17954.0
69	14672.5
70	11391.0
71	9966.0
72	8541.0
73	6841.0
74	5141.0
75	5141.0
76	3996.5
77	2852.0
78	2439.5
79	2027.0
80	1558.0
81	1089.0
82	1089.0
83	904.0
84	719.0
85	601.5
86	484.0
87	443.5
88	403.0
89	403.0
90	293.5
91	184.0
92	141.5
93	99.0
94	86.0
95	73.0
96	73.0
97	44.5
98	16.0
99	13.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1349570.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.566270318496905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.18836765937719	51.93485876626954
2	6.82189657686815	8.127098711651138
3	2.260892663434249	4.040188306536927
4	1.092106820851395	2.6021092043003393
5	0.6128851458965036	1.8253641137331273
6	0.39409627163169414	1.4084907028515166
7	0.2901329944218483	1.209749826183274
8	0.2170687201527275	1.0343979249846018
9	0.15450879119022928	0.8283161180359253
>10	0.8300351764101698	8.90332771463275
>50	0.07231705776571622	2.9850224874081026
>100	0.05641501314216428	6.569337348954135
>500	0.005516118780465621	2.295915173923242
>1k	0.00351025740575085	4.533284324221315
>5k	1.2536633591967323E-4	0.696428847357559
>10k+	1.2536633591967323E-4	1.006110428956508
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13473	0.9983179827648807	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9326	0.6910349222344895	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4984	0.36930281497069434	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	4912	0.3639677823306683	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	4023	0.2980949487614573	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	3988	0.2955015301170002	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	3888	0.28809176256140845	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	3447	0.2554146876412487	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	3186	0.23607519432115415	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	2569	0.19035692850315283	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2311	0.17123972820972605	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2192	0.16242210481857183	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1958	0.14508324873848708	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	1955	0.1448609557118193	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	1758	0.13026371362730352	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	1658	0.12285394607171173	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	1641	0.12159428558726113	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1606	0.11900086694280401	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1563	0.11581466689389955	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	1535	0.11373993197833383	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	1476	0.10936816912053468	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	7.409767555591781E-5	0.0
13	0.0	0.0	0.0	1.4819535111183562E-4	0.0
14	0.0	0.0	0.0	4.4458605333550687E-4	7.409767555591781E-5
15	0.0	0.0	0.0	8.891721066710137E-4	7.409767555591781E-5
16	0.0	0.0	0.0	0.002297027942233452	7.409767555591781E-5
17	0.0	0.0	0.0	0.004519958208910987	7.409767555591781E-5
18	0.0	0.0	0.0	0.005631423342249754	7.409767555591781E-5
19	0.0	0.0	0.0	0.008002548960039123	7.409767555591781E-5
20	0.0	0.0	0.0	0.01015138155116074	7.409767555591781E-5
21	0.0	0.0	0.0	0.016079195595634165	7.409767555591781E-5
22	0.0	0.0	0.0	0.026971553902354083	7.409767555591781E-5
23	0.0	0.0	0.0	0.04431040998243885	1.4819535111183562E-4
24	0.0	0.0	0.0	0.07728387560482228	1.4819535111183562E-4
25	0.0	0.0	0.0	0.10040235037826864	1.4819535111183562E-4
26	0.0	0.0	0.0	0.12789258800951414	1.4819535111183562E-4
27	0.0	0.0	0.0	0.16694206302748282	1.4819535111183562E-4
28	0.0	0.0	0.0	0.23229621286780233	1.4819535111183562E-4
29	0.0	0.0	0.0	0.29987329297479937	1.4819535111183562E-4
30	0.0	0.0	0.0	0.39612617352193663	1.4819535111183562E-4
31	0.0	0.0	0.0	0.5919663300162273	1.4819535111183562E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGAG	200	0.0	18.5	3
GTAGGAC	110	3.8531653E-8	18.5	3
TCGGTAA	50	0.0070355004	18.5	22
TAGGACT	155	2.1827873E-11	17.903227	4
GTATTAG	255	0.0	17.411764	1
TAGAGTG	195	0.0	17.076921	5
CTAGGAC	210	0.0	16.738094	3
TTAGAGT	190	1.8189894E-12	16.552631	4
CGATGCG	195	1.8189894E-12	16.128204	33
TATTAGC	340	0.0	15.779413	2
TTTAGAC	120	1.9360104E-6	15.416666	3
GGAGTGT	325	0.0	15.369231	6
TAAGACA	205	5.456968E-12	15.341464	4
GTACCGT	85	5.365008E-4	15.235295	6
CTATTCA	85	5.365008E-4	15.235295	4
TTAGCTC	305	0.0	15.163934	4
TGCGGCG	220	1.8189894E-12	15.136364	36
TACACCG	135	3.9733277E-7	15.074073	5
ATTAGCT	320	0.0	15.03125	3
TACTTAC	235	0.0	14.957447	31
>>END_MODULE