FastQCFastQC Report
Thu 9 Feb 2017
SRR2727108.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727108.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3351749
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT331520.989095543848898No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219060.6535692260965842No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG192830.5753115761353251No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC176980.5280228322586208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT137140.4091595164196364No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA128760.3841576442627416No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG104000.3102857642383126No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT94880.28307608952818364No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG92660.2764526818684812No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG83760.24989938089039482No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77870.23232646597343654No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC65670.19592755901471143No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC61470.18339678776662574No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA55000.16409343301064608No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54020.16116958638609274No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG53650.16006568510947566No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT50930.15195051896785827No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC48750.14544645198670902No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45980.13718210999690011No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44320.13222947183694245No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT37840.1128962819113245No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT36910.11012161113496267No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC36440.10871935816196261No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT35480.10585518187668588No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT34620.10328935728779214No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA34490.10290150008249424No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC33920.10120089541311118No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC33720.10060419202034519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG10000.019.0555
GTAATAC1700.018.53
CAAGACG10250.018.4097584
GGTTATC4450.018.2921332
CGAACGT3100.017.9032254
ATTAGAG6200.017.6048373
TTAGAGT5650.017.3539834
TATACCG1201.04293576E-716.9583345
ATTCGAA3400.016.8676471
TAGAGTG5650.016.3716815
TCGAACG3550.016.154933
GGTCGTA700.002593884215.85714229
TATCTAG9500.015.77368451
AGGTTAT5400.015.7592591
TCGTTAC2500.015.5423
GTTCGAC1201.9380968E-615.41666711
ACGGACC11250.015.2933328
CACTCTA6900.015.2826099
ACGAACG4750.015.18947315
GACGGAC11450.015.1877747
Produced by FastQC (version 0.11.5)