##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727108.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3351749
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.39509133888009	31.0	30.0	31.0	27.0	34.0
2	30.7775934295796	31.0	31.0	33.0	27.0	34.0
3	31.11390560570019	31.0	31.0	34.0	28.0	34.0
4	35.014987995819496	35.0	35.0	37.0	32.0	37.0
5	34.72350256537706	35.0	35.0	37.0	32.0	37.0
6	34.63961203538809	35.0	35.0	37.0	31.0	37.0
7	34.45162689688279	35.0	35.0	37.0	30.0	37.0
8	34.4839852268174	35.0	35.0	37.0	30.0	37.0
9	35.811122789922514	37.0	35.0	39.0	30.0	39.0
10	35.66420098879719	37.0	35.0	39.0	30.0	39.0
11	35.65992023865749	37.0	35.0	39.0	30.0	39.0
12	35.49608189634725	37.0	35.0	39.0	30.0	39.0
13	35.60383071644088	37.0	35.0	39.0	30.0	39.0
14	36.31406856539675	38.0	35.0	40.0	30.0	41.0
15	36.33547112268848	38.0	35.0	40.0	30.0	41.0
16	36.138858548178874	38.0	34.0	40.0	29.0	41.0
17	36.17981686576173	38.0	34.0	40.0	30.0	41.0
18	36.06178595115565	38.0	34.0	40.0	29.0	41.0
19	35.9823483202352	38.0	34.0	40.0	28.0	41.0
20	36.468857900755694	38.0	35.0	40.0	30.0	41.0
21	36.556441129690796	38.0	35.0	40.0	30.0	41.0
22	36.56377849295995	38.0	35.0	40.0	30.0	41.0
23	36.46972699924726	38.0	35.0	40.0	30.0	41.0
24	36.328505803984726	38.0	35.0	40.0	30.0	41.0
25	36.026875222458486	38.0	34.0	40.0	28.0	41.0
26	35.780531597085584	38.0	34.0	40.0	27.0	41.0
27	35.59666520374885	38.0	34.0	40.0	27.0	41.0
28	35.32501986276419	38.0	34.0	40.0	27.0	41.0
29	35.13917181746008	38.0	34.0	40.0	26.0	41.0
30	34.786589329928944	38.0	33.0	40.0	25.0	41.0
31	34.62754907959994	37.0	33.0	40.0	25.0	41.0
32	34.37171578182018	37.0	33.0	40.0	24.0	41.0
33	34.328059469846934	37.0	33.0	40.0	24.0	41.0
34	34.111142868991685	37.0	33.0	40.0	24.0	41.0
35	33.7140382528644	37.0	32.0	40.0	22.0	41.0
36	33.706469965382254	37.0	32.0	40.0	22.0	41.0
37	33.521835316427335	37.0	31.0	40.0	21.0	41.0
38	33.38001861117882	37.0	31.0	40.0	20.0	41.0
39	33.21171454067712	36.0	31.0	40.0	19.0	41.0
40	32.955875275863434	36.0	31.0	40.0	18.0	41.0
41	32.70870670805004	36.0	30.0	39.0	15.0	41.0
42	32.47971641074555	36.0	30.0	39.0	15.0	41.0
43	32.25901492026998	36.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	37.0
12	24.0
13	39.0
14	73.0
15	169.0
16	320.0
17	682.0
18	1243.0
19	2436.0
20	4630.0
21	8029.0
22	13151.0
23	20493.0
24	29680.0
25	41240.0
26	55509.0
27	71920.0
28	91267.0
29	112896.0
30	137823.0
31	166923.0
32	198875.0
33	235456.0
34	276435.0
35	317746.0
36	370750.0
37	427553.0
38	470501.0
39	295840.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.02147744356753	23.04238772056022	14.997035875896437	24.939098959975823
2	17.93908195392913	26.58588098333139	30.670435047493115	24.804602015246367
3	20.360862343809156	27.008705007445364	30.340637082311357	22.28979556643412
4	16.230690305270475	18.20935875568248	30.94554514672787	34.614405792319175
5	13.232703284165966	41.19962443488459	31.730597965420444	13.837074315528996
6	33.560746941373	36.36829607467624	14.894552068188876	15.176404915761891
7	26.888170922106635	33.24738815466194	20.814058570614925	19.0503823526165
8	29.07434297735302	35.014614757847326	19.641148546624464	16.269893718175197
9	28.804573373483517	14.288390926647551	18.97191585646777	37.93511984340116
10	18.172422815670267	29.84955019006495	30.84291216317212	21.135114831092665
11	34.874508801225865	23.826276967636897	19.279874477474298	22.01933975366294
12	21.26006452153786	29.954331305834653	28.146916729146483	20.638687443481
13	30.655905319879263	20.30751706049588	25.50960707379938	23.526970545825478
14	22.95640276166264	22.671268045429414	25.6443128647163	28.728016328191643
15	26.99757648917028	27.95119801631924	21.896836547128082	23.154388947382394
16	22.88572324478951	26.800604699218226	26.593488951589155	23.7201831044031
17	23.67486348172253	29.114143093650508	25.423502774223252	21.787490650403715
18	24.18322493718951	24.673864301891342	27.793936837155762	23.34897392376338
19	24.72795546444558	27.74775199455568	26.534594326723155	20.98969821427559
20	23.754881406692448	25.520914603092297	27.787641616362084	22.93656237385317
21	23.730356897249763	27.791550023584698	26.827158000196317	21.65093507896922
22	23.931207259254794	27.322421816192083	24.713724088528107	24.032646836025013
23	22.009195795985917	26.996114565858004	26.382971994621318	24.611717643534764
24	22.207838355437715	29.46431847969523	25.88532136505448	22.442521799812575
25	23.421443550814814	27.37003874693481	24.641314131815957	24.56720357043442
26	23.3392476584613	27.485739534792135	26.40967447144759	22.765338335298974
27	24.18092762912736	27.45262249649362	24.945573191787332	23.420876682591686
28	22.628335236319906	28.12227287902525	26.71106935513369	22.538322529521153
29	23.11772152389693	27.220415371198737	27.077952436175863	22.58391066872848
30	22.451800537570087	28.996890876971992	27.128284367355672	21.423024218102253
31	24.02918595634697	27.66125983777425	25.018042818838758	23.29151138704002
32	20.79287560017173	29.01219631899644	26.15524014477218	24.03968793605965
33	22.004272992995595	27.956508676514858	26.93444527021564	23.104773060273907
34	21.75759581042614	28.17558832716889	26.82432365908068	23.242492203324293
35	22.170425052711288	29.07288105404074	27.171992890875778	21.584701002372196
36	23.037614093418092	28.287350872633958	25.96871066419353	22.70632436975442
37	22.06492789287026	28.539428220907947	26.702059133902928	22.693584752318863
38	22.68296343192763	27.771590295096683	27.167517615430032	22.377928657545656
39	22.81507356308602	26.32007945702378	26.929761148582426	23.935085831307774
40	22.459468176167128	27.690349128171594	27.460439310938856	22.389743384722426
41	21.236256056166496	27.911934933075237	27.53365481723124	23.318154193527022
42	22.574124733087114	28.38607544896709	27.540278224890947	21.499521593054848
43	22.18143423030782	26.58919268716124	27.613463896013695	23.615909186517246
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	929.0
1	1410.0
2	1891.0
3	1940.5
4	1990.0
5	1990.0
6	2594.0
7	3198.0
8	4913.0
9	6628.0
10	11126.5
11	15625.0
12	15625.0
13	32428.0
14	49231.0
15	61291.5
16	73352.0
17	56908.5
18	40465.0
19	40465.0
20	45238.0
21	50011.0
22	44429.5
23	38848.0
24	42013.0
25	45178.0
26	45178.0
27	49553.5
28	53929.0
29	56943.0
30	59957.0
31	64430.5
32	68904.0
33	68904.0
34	80054.5
35	91205.0
36	105365.5
37	119526.0
38	134688.5
39	149851.0
40	149851.0
41	160102.0
42	170353.0
43	183078.5
44	195804.0
45	225620.0
46	255436.0
47	255436.0
48	329717.5
49	403999.0
50	392753.0
51	381507.0
52	307336.5
53	233166.0
54	233166.0
55	211093.5
56	189021.0
57	179876.0
58	170731.0
59	158630.5
60	146530.0
61	146530.0
62	124418.0
63	102306.0
64	88882.0
65	75458.0
66	64298.5
67	53139.0
68	53139.0
69	43635.5
70	34132.0
71	30858.5
72	27585.0
73	21879.0
74	16173.0
75	16173.0
76	12369.0
77	8565.0
78	7465.0
79	6365.0
80	4956.5
81	3548.0
82	3548.0
83	3017.5
84	2487.0
85	2073.5
86	1660.0
87	1640.0
88	1620.0
89	1620.0
90	1143.0
91	666.0
92	522.0
93	378.0
94	331.5
95	285.0
96	285.0
97	170.0
98	55.0
99	58.5
100	62.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3351749.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.8016628517028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.18788467392933	40.24320879381208
2	8.099584292585979	7.743471951862854
3	2.78978591841482	4.000692177014798
4	1.3408915241878852	2.5638737823974074
5	0.7889287662092964	1.8856053448173327
6	0.5281648139668876	1.5148293820426497
7	0.3591130041752638	1.2016339125873672
8	0.26824427959021596	1.0258018091895524
9	0.21471268164617144	0.9237260896231755
>10	1.224343355102173	10.596460122082792
>50	0.09597751606326994	3.1803097938217118
>100	0.08160467772821126	8.23640441257042
>500	0.011542801624512834	3.8102679552990737
>1k	0.008155240278111845	7.135642331477713
>5k	6.273261752393728E-4	2.0688492834430687
>10k+	4.391283226675609E-4	3.8692228579579324
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33152	0.989095543848898	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21906	0.6535692260965842	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	19283	0.5753115761353251	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	17698	0.5280228322586208	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13714	0.4091595164196364	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	12876	0.3841576442627416	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	10400	0.3102857642383126	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	9488	0.28307608952818364	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	9266	0.2764526818684812	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	8376	0.24989938089039482	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7787	0.23232646597343654	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	6567	0.19592755901471143	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	6147	0.18339678776662574	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	5500	0.16409343301064608	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5402	0.16116958638609274	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	5365	0.16006568510947566	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	5093	0.15195051896785827	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	4875	0.14544645198670902	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4598	0.13718210999690011	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4432	0.13222947183694245	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3784	0.1128962819113245	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT	3691	0.11012161113496267	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	3644	0.10871935816196261	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	3548	0.10585518187668588	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	3462	0.10328935728779214	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	3449	0.10290150008249424	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	3392	0.10120089541311118	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	3372	0.10060419202034519	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.983516963829929E-5
2	0.0	0.0	0.0	0.0	2.983516963829929E-5
3	0.0	0.0	0.0	0.0	2.983516963829929E-5
4	0.0	0.0	0.0	2.983516963829929E-5	2.983516963829929E-5
5	2.983516963829929E-5	0.0	0.0	2.983516963829929E-5	2.983516963829929E-5
6	2.983516963829929E-5	0.0	0.0	2.983516963829929E-5	2.983516963829929E-5
7	2.983516963829929E-5	0.0	0.0	2.983516963829929E-5	2.983516963829929E-5
8	5.967033927659858E-5	0.0	0.0	2.983516963829929E-5	2.983516963829929E-5
9	5.967033927659858E-5	0.0	0.0	1.4917584819149643E-4	8.950550891489786E-5
10	5.967033927659858E-5	2.983516963829929E-5	0.0	2.0884618746809503E-4	8.950550891489786E-5
11	5.967033927659858E-5	2.983516963829929E-5	0.0	2.685165267446936E-4	8.950550891489786E-5
12	5.967033927659858E-5	2.983516963829929E-5	0.0	3.2818686602129217E-4	8.950550891489786E-5
13	5.967033927659858E-5	5.967033927659858E-5	0.0	3.8785720529789074E-4	8.950550891489786E-5
14	1.1934067855319716E-4	5.967033927659858E-5	0.0	5.370330534893872E-4	1.1934067855319716E-4
15	1.1934067855319716E-4	5.967033927659858E-5	0.0	0.0010442309373404751	1.1934067855319716E-4
16	1.1934067855319716E-4	5.967033927659858E-5	0.0	0.0022376377228724466	1.4917584819149643E-4
17	1.1934067855319716E-4	5.967033927659858E-5	0.0	0.003968077561893805	1.4917584819149643E-4
18	1.1934067855319716E-4	5.967033927659858E-5	0.0	0.004952638159957682	1.7901101782979572E-4
19	1.1934067855319716E-4	5.967033927659858E-5	0.0	0.0075781330881280196	1.7901101782979572E-4
20	1.1934067855319716E-4	5.967033927659858E-5	0.0	0.010322968694851553	1.7901101782979572E-4
21	1.1934067855319716E-4	5.967033927659858E-5	0.0	0.017453574238405083	1.7901101782979572E-4
22	1.1934067855319716E-4	5.967033927659858E-5	0.0	0.027388685727958745	2.0884618746809503E-4
23	1.1934067855319716E-4	5.967033927659858E-5	0.0	0.04564780954659791	3.5802203565959143E-4
24	1.1934067855319716E-4	5.967033927659858E-5	0.0	0.08097265039834427	3.5802203565959143E-4
25	1.1934067855319716E-4	5.967033927659858E-5	0.0	0.10531814882319648	3.5802203565959143E-4
26	1.1934067855319716E-4	5.967033927659858E-5	0.0	0.13330353794392122	3.8785720529789074E-4
27	1.1934067855319716E-4	5.967033927659858E-5	0.0	0.17289480805394436	3.8785720529789074E-4
28	1.1934067855319716E-4	5.967033927659858E-5	0.0	0.24190355542733064	3.8785720529789074E-4
29	1.1934067855319716E-4	5.967033927659858E-5	0.0	0.3139256549341851	3.8785720529789074E-4
30	1.1934067855319716E-4	5.967033927659858E-5	0.0	0.4128292422851472	3.8785720529789074E-4
31	1.1934067855319716E-4	5.967033927659858E-5	0.0	0.6336393327782003	3.8785720529789074E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1000	0.0	19.055	5
GTAATAC	170	0.0	18.5	3
CAAGACG	1025	0.0	18.409758	4
GGTTATC	445	0.0	18.292133	2
CGAACGT	310	0.0	17.903225	4
ATTAGAG	620	0.0	17.604837	3
TTAGAGT	565	0.0	17.353983	4
TATACCG	120	1.04293576E-7	16.958334	5
ATTCGAA	340	0.0	16.867647	1
TAGAGTG	565	0.0	16.371681	5
TCGAACG	355	0.0	16.15493	3
GGTCGTA	70	0.0025938842	15.857142	29
TATCTAG	950	0.0	15.7736845	1
AGGTTAT	540	0.0	15.759259	1
TCGTTAC	250	0.0	15.54	23
GTTCGAC	120	1.9380968E-6	15.416667	11
ACGGACC	1125	0.0	15.293332	8
CACTCTA	690	0.0	15.282609	9
ACGAACG	475	0.0	15.189473	15
GACGGAC	1145	0.0	15.187774	7
>>END_MODULE