FastQCFastQC Report
Thu 9 Feb 2017
SRR2727107.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727107.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2594861
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT352131.3570283726180323No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172450.6645828042426936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT152160.5863897912065424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT94900.3657228653095484No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49230.18972114498618614No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42310.16305304985507896No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG30730.1184263819911741No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA28280.10898464310805088No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26770.10316544893926882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG6850.018.905115
CAAGACG7050.017.8439714
TAGAGTG4450.017.0449455
CGCAAGA7750.016.4709682
TTAGAGT3950.016.3924054
CGAACGT2550.015.9607844
CGAACGA3250.015.9384616
TATACAG4750.015.5789475
AGGTTAT4050.015.5308641
TACGACG6450.015.4883715
CGTCGTA5500.015.47272810
ACGAACG3650.015.20548115
TCGAACG2800.015.1964293
AACGAAC3550.015.11267714
GCGCAAG7850.015.0828031
CGCGCTA5300.015.00943424
ATACCGT6200.014.9193556
CGCGCGC5350.014.86915922
TACCGTC5850.014.8632477
CTACGTA750.004106255714.833
Produced by FastQC (version 0.11.5)