##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727107.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2594861
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.39894391260264	31.0	30.0	31.0	27.0	34.0
2	30.799021219248353	31.0	31.0	33.0	27.0	34.0
3	31.109112588304345	31.0	31.0	34.0	28.0	34.0
4	35.03853886585833	35.0	35.0	37.0	32.0	37.0
5	34.75393364037611	35.0	35.0	37.0	32.0	37.0
6	34.62693146183938	35.0	35.0	37.0	31.0	37.0
7	34.4848857029336	35.0	35.0	37.0	30.0	37.0
8	34.48980812459704	35.0	35.0	37.0	30.0	37.0
9	35.79552623435321	37.0	35.0	39.0	30.0	39.0
10	35.62384960119251	37.0	35.0	39.0	30.0	39.0
11	35.624485473403006	37.0	35.0	39.0	30.0	39.0
12	35.43257615725852	37.0	34.0	39.0	30.0	39.0
13	35.566707812094755	37.0	35.0	39.0	30.0	39.0
14	36.23926869300514	38.0	34.0	40.0	30.0	41.0
15	36.26360410056647	38.0	34.0	40.0	30.0	41.0
16	36.12516277365146	38.0	34.0	40.0	29.0	41.0
17	36.127935947243415	38.0	34.0	40.0	29.0	41.0
18	36.01670031651021	38.0	34.0	40.0	29.0	41.0
19	35.971465138209716	38.0	34.0	40.0	27.0	41.0
20	36.42800674101618	38.0	35.0	40.0	30.0	41.0
21	36.515093872080236	38.0	35.0	40.0	30.0	41.0
22	36.47891736782818	38.0	35.0	40.0	30.0	41.0
23	36.389620869865475	38.0	35.0	40.0	30.0	41.0
24	36.22869201857055	38.0	35.0	40.0	29.0	41.0
25	35.89963200340981	38.0	34.0	40.0	27.0	41.0
26	35.66337349091146	38.0	34.0	40.0	27.0	41.0
27	35.47759436825325	38.0	34.0	40.0	27.0	41.0
28	35.20728008166911	38.0	34.0	40.0	26.0	41.0
29	35.016158090934354	38.0	33.0	40.0	25.0	41.0
30	34.66576437042293	38.0	33.0	40.0	25.0	41.0
31	34.493380184911636	37.0	33.0	40.0	24.0	41.0
32	34.30350103531557	37.0	33.0	40.0	24.0	41.0
33	34.19544206799517	37.0	33.0	40.0	24.0	41.0
34	34.0008605470582	37.0	32.0	40.0	23.0	41.0
35	33.550411370782484	37.0	31.0	40.0	21.0	41.0
36	33.51053023649436	37.0	31.0	40.0	21.0	41.0
37	33.37786956603841	36.0	31.0	40.0	20.0	41.0
38	33.22054745899684	36.0	31.0	40.0	19.0	41.0
39	33.066696443470384	36.0	31.0	40.0	18.0	41.0
40	32.788131618610784	36.0	30.0	39.0	16.0	41.0
41	32.510638912835795	36.0	30.0	39.0	15.0	40.0
42	32.286318226679576	36.0	30.0	39.0	15.0	40.0
43	32.08294471264549	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	9.0
11	10.0
12	11.0
13	33.0
14	77.0
15	149.0
16	238.0
17	475.0
18	1045.0
19	2015.0
20	3756.0
21	6706.0
22	10924.0
23	16814.0
24	24403.0
25	33353.0
26	44886.0
27	58033.0
28	73225.0
29	89567.0
30	109408.0
31	130976.0
32	155978.0
33	183803.0
34	214134.0
35	244459.0
36	285626.0
37	322526.0
38	347874.0
39	234346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.83085953351644	24.215362595530166	16.122019638046122	23.831758232907273
2	18.608665358182964	25.980081399350485	31.89280658964006	23.51844665282649
3	19.451485069913186	26.61953761685115	31.38341514246813	22.54556217076753
4	15.105664619415066	17.796791427363548	32.4910274577328	34.606516495488584
5	12.302431613870647	42.15944514947043	31.95042046568198	13.587702770976943
6	32.254983985654725	38.979197729666446	14.525672088023212	14.24014619665562
7	27.088117629422154	34.13955506672612	20.125856452426547	18.646470851425182
8	29.208655107152175	35.56529617578745	18.506270663438233	16.71977805362214
9	28.566347099131704	14.07878880602853	18.941322868546717	38.41354122629305
10	17.418929183489983	30.852789417236608	30.665881525060495	21.062399874212915
11	35.71289560404199	24.005678916905378	19.360381924118478	20.92104355493416
12	20.849517565680785	30.229326349272657	28.201549138855608	20.719606946190954
13	30.382128368340343	20.974957810842277	25.665844914236253	22.977068906581124
14	22.34377872263678	22.84561677870221	26.379139383573918	28.43146511508709
15	25.49388965343423	29.882795263407175	22.052857551907405	22.570457531251193
16	22.929474835068238	27.527717284278424	25.40698711799977	24.135820762653566
17	22.649729600159702	29.277406381305205	25.482521029064753	22.59034298947034
18	22.81135675475488	26.061473042293983	27.8133587887752	23.31381141417594
19	23.981939687713524	26.87407918959821	27.695394859300748	21.44858626338752
20	23.462682586851475	25.985168377034455	27.913595371775212	22.63855366433886
21	24.30796871200423	26.89639252353016	27.44112305052178	21.35451571394383
22	23.814339188110655	26.934968770966922	26.49055190239477	22.760140138527653
23	22.559088906881716	26.948842346468656	27.423819618854345	23.068249127795283
24	22.421123906058938	28.00963134441498	27.111471481516737	22.457773268009344
25	22.77393663860993	27.274717219920454	26.85072533750363	23.10062080396599
26	22.92581375264417	27.60290435595587	27.2244640464364	22.246817844963566
27	23.232805148329717	27.768539432362658	26.506467976512038	22.492187442795586
28	22.366092056568736	27.864190027905156	27.63304084496241	22.136677070563703
29	22.69493433366951	27.751929679470305	27.555734199249983	21.997401787610205
30	22.43526724552876	28.51382020077376	27.420119998720548	21.63079255497693
31	22.781297341167793	27.95089987479098	26.906335252639735	22.361467531401487
32	21.568900993155317	28.751173954982562	26.896431061239888	22.783493990622233
33	21.805406917750123	27.91028112873869	27.59535096484937	22.688960988661822
34	22.053474155263036	28.077110874147017	27.756978119444547	22.112436851145397
35	22.38767317401587	28.29700704584947	27.54856618524075	21.76675359489391
36	21.897974496514458	28.39127028384179	27.296336875077316	22.414418344566435
37	21.820205398285303	28.297585111495376	27.508641117963545	22.37356837225578
38	21.943949984218808	27.49307188323382	27.994177722814438	22.56880040973293
39	21.992815800152687	27.259841663965815	27.941535211327313	22.805807324554188
40	21.854581035361818	27.555811274669434	28.44614798249309	22.14345970747566
41	20.95854074649856	27.985545275835584	28.69741384991335	22.358500127752507
42	21.54466077373701	27.962923640225817	28.5421068797134	21.950308706323767
43	20.9995063319384	27.198258403822017	28.64920317504483	23.15303208919476
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1337.0
1	1402.0
2	1467.0
3	1484.0
4	1501.0
5	1501.0
6	2018.0
7	2535.0
8	3725.0
9	4915.0
10	8775.0
11	12635.0
12	12635.0
13	26282.5
14	39930.0
15	54102.0
16	68274.0
17	51481.5
18	34689.0
19	34689.0
20	38538.0
21	42387.0
22	37061.5
23	31736.0
24	36007.5
25	40279.0
26	40279.0
27	44908.0
28	49537.0
29	53673.5
30	57810.0
31	62977.5
32	68145.0
33	68145.0
34	77410.0
35	86675.0
36	97184.0
37	107693.0
38	119004.5
39	130316.0
40	130316.0
41	140144.5
42	149973.0
43	157948.5
44	165924.0
45	179660.5
46	193397.0
47	193397.0
48	210764.5
49	228132.0
50	222068.0
51	216004.0
52	198656.5
53	181309.0
54	181309.0
55	169509.0
56	157709.0
57	149167.0
58	140625.0
59	129418.5
60	118212.0
61	118212.0
62	100460.0
63	82708.0
64	71871.5
65	61035.0
66	51402.5
67	41770.0
68	41770.0
69	33912.0
70	26054.0
71	22632.0
72	19210.0
73	15526.0
74	11842.0
75	11842.0
76	9151.0
77	6460.0
78	5561.0
79	4662.0
80	3690.5
81	2719.0
82	2719.0
83	2290.0
84	1861.0
85	1512.5
86	1164.0
87	1118.0
88	1072.0
89	1072.0
90	808.0
91	544.0
92	427.5
93	311.0
94	263.0
95	215.0
96	215.0
97	128.5
98	42.0
99	44.0
100	46.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2594861.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.034868856810085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.92420203825016	48.14751392839402
2	7.402839709726082	8.296343046049742
3	2.354455023180466	3.957947353595255
4	1.170957992041471	2.6245791008350996
5	0.6899721768709105	1.9331250222904626
6	0.46698392399164135	1.5700429763466115
7	0.34004947251686074	1.3338239318116814
8	0.24993717238752156	1.1204157341741365
9	0.18687003350541673	0.9424114038669362
>10	1.0596552091326799	10.780554492617354
>50	0.08066165591556072	3.13143420324608
>100	0.06208826081911896	7.165038651186968
>500	0.00759760050516494	2.768603158764399
>1k	0.003453454775072775	3.241705905847899
>5k	6.90690955014555E-5	0.367289354534994
>10k+	2.0720728650436648E-4	2.619171736438481
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35213	1.3570283726180323	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17245	0.6645828042426936	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15216	0.5863897912065424	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9490	0.3657228653095484	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4923	0.18972114498618614	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4231	0.16305304985507896	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	3073	0.1184263819911741	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	2828	0.10898464310805088	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2677	0.10316544893926882	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.8537709727033546E-5
2	0.0	0.0	0.0	0.0	3.8537709727033546E-5
3	0.0	0.0	0.0	0.0	3.8537709727033546E-5
4	0.0	0.0	0.0	0.0	3.8537709727033546E-5
5	0.0	0.0	0.0	0.0	3.8537709727033546E-5
6	3.8537709727033546E-5	0.0	0.0	0.0	3.8537709727033546E-5
7	3.8537709727033546E-5	0.0	0.0	0.0	3.8537709727033546E-5
8	3.8537709727033546E-5	0.0	0.0	0.0	3.8537709727033546E-5
9	3.8537709727033546E-5	0.0	0.0	0.0	7.707541945406709E-5
10	3.8537709727033546E-5	0.0	0.0	3.8537709727033546E-5	7.707541945406709E-5
11	3.8537709727033546E-5	0.0	0.0	1.1561312918110065E-4	7.707541945406709E-5
12	3.8537709727033546E-5	0.0	0.0	1.5415083890813418E-4	7.707541945406709E-5
13	3.8537709727033546E-5	0.0	0.0	1.9268854863516773E-4	7.707541945406709E-5
14	3.8537709727033546E-5	0.0	0.0	4.2391480699736906E-4	7.707541945406709E-5
15	3.8537709727033546E-5	0.0	0.0	6.166033556325367E-4	7.707541945406709E-5
16	3.8537709727033546E-5	0.0	0.0	0.002312262583622013	7.707541945406709E-5
17	3.8537709727033546E-5	0.0	0.0	0.003969384101884455	7.707541945406709E-5
18	3.8537709727033546E-5	0.0	0.0	0.004431836618608858	1.1561312918110065E-4
19	3.8537709727033546E-5	0.0	0.0	0.006011882717417234	1.1561312918110065E-4
20	3.8537709727033546E-5	0.0	0.0	0.007399240267590442	1.1561312918110065E-4
21	3.8537709727033546E-5	0.0	0.0	0.01152277520838303	1.1561312918110065E-4
22	3.8537709727033546E-5	0.0	0.0	0.018074185861978734	1.1561312918110065E-4
23	3.8537709727033546E-5	0.0	0.0	0.029905262748178032	1.1561312918110065E-4
24	3.8537709727033546E-5	0.0	0.0	0.04851897654633524	1.1561312918110065E-4
25	3.8537709727033546E-5	0.0	0.0	0.05599529223337975	1.1561312918110065E-4
26	3.8537709727033546E-5	0.0	0.0	0.06528288027759484	1.1561312918110065E-4
27	3.8537709727033546E-5	0.0	0.0	0.07965744600577834	1.1561312918110065E-4
28	3.8537709727033546E-5	0.0	0.0	0.11148959424030805	1.1561312918110065E-4
29	3.8537709727033546E-5	0.0	0.0	0.15765777049329424	1.1561312918110065E-4
30	3.8537709727033546E-5	0.0	0.0	0.23681422627262116	1.1561312918110065E-4
31	3.8537709727033546E-5	0.0	0.0	0.4530493155510064	1.1561312918110065E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	685	0.0	18.90511	5
CAAGACG	705	0.0	17.843971	4
TAGAGTG	445	0.0	17.044945	5
CGCAAGA	775	0.0	16.470968	2
TTAGAGT	395	0.0	16.392405	4
CGAACGT	255	0.0	15.960784	4
CGAACGA	325	0.0	15.93846	16
TATACAG	475	0.0	15.578947	5
AGGTTAT	405	0.0	15.530864	1
TACGACG	645	0.0	15.488371	5
CGTCGTA	550	0.0	15.472728	10
ACGAACG	365	0.0	15.205481	15
TCGAACG	280	0.0	15.196429	3
AACGAAC	355	0.0	15.112677	14
GCGCAAG	785	0.0	15.082803	1
CGCGCTA	530	0.0	15.009434	24
ATACCGT	620	0.0	14.919355	6
CGCGCGC	535	0.0	14.869159	22
TACCGTC	585	0.0	14.863247	7
CTACGTA	75	0.0041062557	14.8	33
>>END_MODULE