FastQCFastQC Report
Thu 9 Feb 2017
SRR2727106.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727106.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515605
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT161761.0672965581401488No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100110.6605283038786491No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT67310.444113076956067No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG52880.34890357316055304No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC48380.3192124597108085No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37360.24650222188498983No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA36520.24095988070770416No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG31160.20559446557645294No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT29870.19708301305419287No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG27260.17986216725334106No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25560.16864552439454872No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG24070.15881446683007777No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC21840.1441008706094266No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21630.1427152853151052No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18860.12443875548048468No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC18500.12206346640450513No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA18320.12087582186651535No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG17320.11427779665546102No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT16100.10622820589797474No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15440.10187350925867887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC2700.021.2407427
ATACCGT2900.021.0517236
CCGTCGT2750.020.8545449
CGTCGTA3000.019.73333410
ACCGTCG3050.019.4098368
TGTGCGA609.238668E-418.510
CGTTAAT500.007035890618.51
TCGTAAG500.007035890618.51
GGTTATC2350.017.3191492
GTCGTAG3250.017.07692311
CGAACGA1655.4569682E-1116.81818216
GTTATCT2450.016.6122443
TCAGATA4350.016.5862082
CCGATAA2050.016.2439029
TAATACT2400.016.18754
AATTAAC1951.8189894E-1216.1282062
AAGTACT6100.016.073774
AGGTTAT2550.015.9607841
AAGACGG3750.015.7866685
GTAAGAC1302.59035E-715.6538463
Produced by FastQC (version 0.11.5)