##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727106.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1515605
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.403077318958434	31.0	30.0	31.0	27.0	34.0
2	30.79273689384767	31.0	31.0	33.0	27.0	34.0
3	31.110756430600322	31.0	31.0	34.0	28.0	34.0
4	35.029391563105165	35.0	35.0	37.0	32.0	37.0
5	34.73650588378898	35.0	35.0	37.0	32.0	37.0
6	34.633384687962895	35.0	35.0	37.0	31.0	37.0
7	34.467948443031	35.0	35.0	37.0	30.0	37.0
8	34.478592377301474	35.0	35.0	37.0	30.0	37.0
9	35.81066702735871	37.0	35.0	39.0	30.0	39.0
10	35.65604032713009	37.0	35.0	39.0	30.0	39.0
11	35.656784584373895	37.0	35.0	39.0	30.0	39.0
12	35.48124148442371	37.0	35.0	39.0	30.0	39.0
13	35.60555751663527	37.0	35.0	39.0	30.0	39.0
14	36.31891884758892	38.0	35.0	40.0	30.0	41.0
15	36.33482470696521	38.0	35.0	40.0	30.0	41.0
16	36.17284120862626	38.0	34.0	40.0	30.0	41.0
17	36.18046984537528	38.0	34.0	40.0	30.0	41.0
18	36.058584525651476	38.0	34.0	40.0	29.0	41.0
19	36.0102665272284	38.0	34.0	40.0	28.0	41.0
20	36.46434988008089	38.0	35.0	40.0	30.0	41.0
21	36.56419317698213	38.0	35.0	40.0	30.0	41.0
22	36.55432187146387	38.0	35.0	40.0	30.0	41.0
23	36.441987853035585	38.0	35.0	40.0	30.0	41.0
24	36.31160757585255	38.0	35.0	40.0	30.0	41.0
25	35.98953157320014	38.0	34.0	40.0	28.0	41.0
26	35.776055106706565	38.0	34.0	40.0	27.0	41.0
27	35.609006304413086	38.0	34.0	40.0	27.0	41.0
28	35.34804121126547	38.0	34.0	40.0	27.0	41.0
29	35.182422860837754	38.0	34.0	40.0	26.0	41.0
30	34.83251968685772	38.0	33.0	40.0	25.0	41.0
31	34.658810837916214	37.0	33.0	40.0	25.0	41.0
32	34.44649892287239	37.0	33.0	40.0	24.0	41.0
33	34.38695240514514	37.0	33.0	40.0	24.0	41.0
34	34.185843936909684	37.0	33.0	40.0	24.0	41.0
35	33.77152820160926	37.0	32.0	40.0	22.0	41.0
36	33.75470917554376	37.0	32.0	40.0	22.0	41.0
37	33.585288383186914	37.0	32.0	40.0	21.0	41.0
38	33.443521893897156	37.0	31.0	40.0	21.0	41.0
39	33.28685970289092	36.0	31.0	40.0	19.0	41.0
40	33.0305930634961	36.0	31.0	40.0	18.0	41.0
41	32.77629857383685	36.0	31.0	39.0	16.0	41.0
42	32.54633034332824	36.0	30.0	39.0	15.0	41.0
43	32.3414708977603	36.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	11.0
12	12.0
13	12.0
14	33.0
15	65.0
16	131.0
17	241.0
18	499.0
19	994.0
20	1978.0
21	3657.0
22	5893.0
23	8802.0
24	13034.0
25	18570.0
26	24791.0
27	32457.0
28	41237.0
29	51166.0
30	62727.0
31	75224.0
32	89831.0
33	106519.0
34	124064.0
35	143210.0
36	167432.0
37	192684.0
38	212110.0
39	138215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.69471927052233	23.366774324444695	15.161470171977525	24.777036233055448
2	18.21338673335071	26.250573203440208	31.0766987440659	24.459341319143178
3	20.057666740344615	26.78461736402295	30.60038730407989	22.557328591552547
4	15.850897826280594	18.021648120717472	31.736963126936107	34.39049092606583
5	13.119843230920985	41.143899630840494	31.918738721500656	13.81751841673787
6	33.272587514556896	37.02092563695686	14.761431903431301	14.945054945054945
7	27.01205129304799	33.483592360806405	20.69206686438749	18.81228948175811
8	28.78955928490603	35.248300183755006	19.35002853645904	16.612111994879932
9	28.421059576868647	14.362251378162515	19.110520221297765	38.10616882367108
10	17.81057729421584	29.977137842643696	30.99145225833908	21.22083260480138
11	34.99625562069273	23.821642182494777	19.59085645666252	21.591245740149972
12	21.015897941746037	29.991719478360128	28.368077434423878	20.62430514546996
13	30.28750894857169	20.590787177397804	25.726492060926166	23.39521181310434
14	22.656694851231027	22.604438491559474	25.974841729870253	28.76402492733925
15	26.168295829058362	28.61471161681309	22.03417117256805	23.182821381560498
16	22.93156858152355	26.9501618165683	26.208543782845794	23.909725819062356
17	23.123241213904677	29.1761375820217	25.61927415124653	22.081347052827088
18	23.62766024128978	25.203730523454333	27.78336044022024	23.385248795035647
19	24.333450998116263	27.411891620837885	26.97563019388297	21.27902718716288
20	23.6008722589329	25.86221343951755	27.906347630154293	22.630566671395254
21	23.92344971150135	27.44211057630451	26.984801448926337	21.649638263267803
22	23.858921024937235	27.224771625852384	25.490546679378863	23.42576066983152
23	22.180713312505567	27.021024607335026	26.77775541780345	24.020506662355956
24	22.35714450664916	28.996605316028912	26.25110104545709	22.395149131864834
25	23.184734808871703	27.6097663969174	25.450034804582987	23.755463989627902
26	23.087809818521315	27.559951306573943	26.65740743795382	22.69483143695092
27	23.670019563144752	27.61174580448072	25.585558242418045	23.132676389956487
28	22.557460552056767	28.032105990676992	27.161892445591036	22.248541011675204
29	22.817356765120202	27.541872717495654	27.21461066702736	22.42615985035679
30	22.433351697836837	28.920002243328575	27.18683298088882	21.45981307794577
31	23.434536043362222	27.853299507457418	25.790690846229726	22.921473602950638
32	21.10272795352351	28.83112684373567	26.476093705154046	23.59005149758677
33	21.847776960355766	28.081393239003567	27.240408945602578	22.830420855038085
34	21.74775089815618	28.23374164112681	27.142560231722644	22.875947228994363
35	22.214561181838278	28.836537224408737	27.21975712669198	21.72914446706101
36	22.53469736507863	28.358312357111515	26.345518786227284	22.76147149158257
37	21.952553600707308	28.523394947892093	26.89625595059399	22.62779550080661
38	22.179591648219688	27.880285430570627	27.513171307827566	22.426951613382116
39	22.439817762543672	26.8406345980648	27.246083247284087	23.47346439210744
40	22.223336555368977	27.777422217530294	27.650542192721716	22.34869903437901
41	21.038595148472062	27.980245512518103	27.928582975115546	23.052576363894286
42	22.152671705358586	28.315425193239662	27.847691186028023	21.684211915373726
43	21.683816033861063	26.99166339514583	27.975560914618253	23.348959656374845
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	435.0
1	651.0
2	867.0
3	865.0
4	863.0
5	863.0
6	1086.0
7	1309.0
8	2106.0
9	2903.0
10	4919.5
11	6936.0
12	6936.0
13	14652.0
14	22368.0
15	28532.5
16	34697.0
17	26696.5
18	18696.0
19	18696.0
20	21043.5
21	23391.0
22	20807.0
23	18223.0
24	19849.5
25	21476.0
26	21476.0
27	23969.0
28	26462.0
29	28262.0
30	30062.0
31	32493.5
32	34925.0
33	34925.0
34	40170.5
35	45416.0
36	51761.5
37	58107.0
38	65208.5
39	72310.0
40	72310.0
41	77193.0
42	82076.0
43	87550.5
44	93025.0
45	104126.5
46	115228.0
47	115228.0
48	139156.0
49	163084.0
50	157784.5
51	152485.0
52	129063.5
53	105642.0
54	105642.0
55	97150.5
56	88659.0
57	84019.0
58	79379.0
59	73360.5
60	67342.0
61	67342.0
62	57067.0
63	46792.0
64	40387.5
65	33983.0
66	28830.0
67	23677.0
68	23677.0
69	19294.0
70	14911.0
71	13378.0
72	11845.0
73	9405.0
74	6965.0
75	6965.0
76	5332.5
77	3700.0
78	3210.5
79	2721.0
80	2164.5
81	1608.0
82	1608.0
83	1352.0
84	1096.0
85	888.5
86	681.0
87	675.0
88	669.0
89	669.0
90	473.5
91	278.0
92	205.5
93	133.0
94	134.5
95	136.0
96	136.0
97	78.0
98	20.0
99	22.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1515605.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.51257094847622
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.64485009132454	48.96523238106064
2	7.066647204553384	7.987088030303484
3	2.265512028056098	3.8408972766043945
4	1.1444360304560328	2.5870008946855614
5	0.6649806699678672	1.8789883645462173
6	0.4296618287949859	1.456877675017721
7	0.3185243277979356	1.2600440071447572
8	0.23010098973173992	1.0402878806023639
9	0.17269176734214386	0.8783330178726588
>10	0.9009100942236151	9.362382837447237
>50	0.07916511949685019	3.1199191874102845
>100	0.07037771302505058	8.120309334228413
>500	0.008369821371952373	3.296677625564881
>1k	0.0033007746255586824	3.6606878619210805
>5k	2.3576961611133446E-4	0.800702604459216
>10k+	2.3576961611133446E-4	1.7445710211310004
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16176	1.0672965581401488	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10011	0.6605283038786491	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6731	0.444113076956067	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	5288	0.34890357316055304	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	4838	0.3192124597108085	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3736	0.24650222188498983	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	3652	0.24095988070770416	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	3116	0.20559446557645294	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	2987	0.19708301305419287	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	2726	0.17986216725334106	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2556	0.16864552439454872	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	2407	0.15881446683007777	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	2184	0.1441008706094266	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2163	0.1427152853151052	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1886	0.12443875548048468	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	1850	0.12206346640450513	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	1832	0.12087582186651535	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	1732	0.11427779665546102	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1610	0.10622820589797474	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1544	0.10187350925867887	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.598025211054331E-5
2	6.598025211054331E-5	0.0	0.0	0.0	6.598025211054331E-5
3	6.598025211054331E-5	0.0	0.0	0.0	6.598025211054331E-5
4	6.598025211054331E-5	0.0	0.0	6.598025211054331E-5	6.598025211054331E-5
5	6.598025211054331E-5	0.0	0.0	6.598025211054331E-5	6.598025211054331E-5
6	6.598025211054331E-5	0.0	0.0	6.598025211054331E-5	6.598025211054331E-5
7	6.598025211054331E-5	0.0	0.0	6.598025211054331E-5	6.598025211054331E-5
8	6.598025211054331E-5	0.0	0.0	6.598025211054331E-5	6.598025211054331E-5
9	6.598025211054331E-5	0.0	0.0	6.598025211054331E-5	6.598025211054331E-5
10	6.598025211054331E-5	0.0	0.0	1.3196050422108663E-4	6.598025211054331E-5
11	6.598025211054331E-5	0.0	0.0	1.3196050422108663E-4	6.598025211054331E-5
12	6.598025211054331E-5	0.0	0.0	1.9794075633162994E-4	6.598025211054331E-5
13	6.598025211054331E-5	0.0	0.0	3.958815126632599E-4	6.598025211054331E-5
14	6.598025211054331E-5	0.0	0.0	5.938222689948899E-4	1.3196050422108663E-4
15	6.598025211054331E-5	0.0	0.0	9.897037816581496E-4	1.3196050422108663E-4
16	6.598025211054331E-5	0.0	0.0	0.0024412693280901026	1.3196050422108663E-4
17	6.598025211054331E-5	0.0	0.0	0.004552637395627489	1.3196050422108663E-4
18	6.598025211054331E-5	0.0	0.0	0.005674301681506725	3.2990126055271657E-4
19	6.598025211054331E-5	0.0	0.0	0.008313511765928457	3.2990126055271657E-4
20	6.598025211054331E-5	0.0	0.0	0.01055684033768693	3.2990126055271657E-4
21	6.598025211054331E-5	0.0	0.0	0.015967221010751483	3.2990126055271657E-4
22	6.598025211054331E-5	0.0	0.0	0.025402397062559177	3.2990126055271657E-4
23	6.598025211054331E-5	0.0	0.0	0.04480059118305891	3.2990126055271657E-4
24	6.598025211054331E-5	0.0	0.0	0.08386090043250055	3.2990126055271657E-4
25	6.598025211054331E-5	0.0	0.0	0.10807565295706995	3.2990126055271657E-4
26	6.598025211054331E-5	0.0	0.0	0.1361832403561614	3.2990126055271657E-4
27	6.598025211054331E-5	0.0	0.0	0.175969332378819	3.2990126055271657E-4
28	6.598025211054331E-5	0.0	0.0	0.2457104588596633	3.2990126055271657E-4
29	6.598025211054331E-5	0.0	0.0	0.31175669122231714	3.958815126632599E-4
30	6.598025211054331E-5	0.0	0.0	0.4129044177077801	3.958815126632599E-4
31	6.598025211054331E-5	0.0	0.0	0.6209401526123232	3.958815126632599E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTC	270	0.0	21.240742	7
ATACCGT	290	0.0	21.051723	6
CCGTCGT	275	0.0	20.854544	9
CGTCGTA	300	0.0	19.733334	10
ACCGTCG	305	0.0	19.409836	8
TGTGCGA	60	9.238668E-4	18.5	10
CGTTAAT	50	0.0070358906	18.5	1
TCGTAAG	50	0.0070358906	18.5	1
GGTTATC	235	0.0	17.319149	2
GTCGTAG	325	0.0	17.076923	11
CGAACGA	165	5.4569682E-11	16.818182	16
GTTATCT	245	0.0	16.612244	3
TCAGATA	435	0.0	16.586208	2
CCGATAA	205	0.0	16.243902	9
TAATACT	240	0.0	16.1875	4
AATTAAC	195	1.8189894E-12	16.128206	2
AAGTACT	610	0.0	16.07377	4
AGGTTAT	255	0.0	15.960784	1
AAGACGG	375	0.0	15.786668	5
GTAAGAC	130	2.59035E-7	15.653846	3
>>END_MODULE