FastQCFastQC Report
Thu 9 Feb 2017
SRR2727105.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727105.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185492
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT199480.9127464204856389No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129560.5928184591844765No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG84870.3883336109214767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82720.37849600913661546No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC81330.3721358851919842No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA60380.2762764631488013No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG51230.2344094602039266No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46580.21313278657620344No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT46010.21052467819603093No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG43340.1983077494678544No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG41310.18901922313145048No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC35130.1607418375358958No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC33720.1542902010165217No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31590.1445441118064033No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG31050.14207327228834515No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA28200.12903273038748253No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27870.12752277290422476No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25600.11713609567090613No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23960.1096320645419887No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC23490.10748151903553066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCG450.00382689520.5555553
CGTTAAT555.145134E-420.1818181
CGAACGT1551.8189894E-1219.0967734
ACGTCTG2000.016.6500027
TAATACG904.4489076E-516.4444454
GGTTATC2750.016.1454542
TCGAACG1851.8189894E-1116.03
AGGTTAT2900.015.9482761
CTAGAAC1902.7284841E-1115.5789483
ATACCGT4700.015.3510656
GTTATCT3350.014.9104483
ACGAACG2500.014.80000115
TACACCG1001.09408E-414.85
TATCTAG4650.014.7204291
GTAGTAC1152.2115366E-514.4782613
ATTCGAA2059.276846E-1114.4390251
TCTATAC2059.276846E-1114.4390253
TAGAGTC5900.014.4237295
TACCGTC4500.014.3888897
ACCGTCG4950.014.2020218
Produced by FastQC (version 0.11.5)