##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727105.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2185492
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.399678424812354	31.0	30.0	31.0	27.0	34.0
2	30.791522000538095	31.0	31.0	33.0	27.0	34.0
3	31.129679724290916	31.0	31.0	34.0	28.0	34.0
4	35.03849018893686	35.0	35.0	37.0	32.0	37.0
5	34.74232072229045	35.0	35.0	37.0	32.0	37.0
6	34.639817029758056	35.0	35.0	37.0	31.0	37.0
7	34.487271058416134	35.0	35.0	37.0	30.0	37.0
8	34.488528441193104	35.0	35.0	37.0	30.0	37.0
9	35.83368138615927	37.0	35.0	39.0	30.0	39.0
10	35.675376528488776	37.0	35.0	39.0	30.0	39.0
11	35.66912393181947	37.0	35.0	39.0	30.0	39.0
12	35.49937176617439	37.0	35.0	39.0	30.0	39.0
13	35.623149844520135	37.0	35.0	39.0	30.0	39.0
14	36.35432342008115	38.0	35.0	40.0	30.0	41.0
15	36.362874812628	38.0	35.0	40.0	30.0	41.0
16	36.194280281053416	38.0	34.0	40.0	30.0	41.0
17	36.20155919124847	38.0	34.0	40.0	30.0	41.0
18	36.08588317870758	38.0	34.0	40.0	29.0	41.0
19	36.01854548083452	38.0	34.0	40.0	28.0	41.0
20	36.4898288348802	38.0	35.0	40.0	30.0	41.0
21	36.58647755288054	38.0	35.0	40.0	30.0	41.0
22	36.57682846699965	38.0	35.0	40.0	30.0	41.0
23	36.48371671001312	38.0	35.0	40.0	30.0	41.0
24	36.35985032203275	38.0	35.0	40.0	30.0	41.0
25	36.05451266808572	38.0	34.0	40.0	29.0	41.0
26	35.83273789151367	38.0	34.0	40.0	27.0	41.0
27	35.673428225772504	38.0	34.0	40.0	27.0	41.0
28	35.4172172673247	38.0	34.0	40.0	27.0	41.0
29	35.25734525681174	38.0	34.0	40.0	26.0	41.0
30	34.908615542861746	38.0	33.0	40.0	25.0	41.0
31	34.74518323562841	38.0	33.0	40.0	25.0	41.0
32	34.54066178233551	37.0	33.0	40.0	24.0	41.0
33	34.476689001835744	37.0	33.0	40.0	24.0	41.0
34	34.27870063125374	37.0	33.0	40.0	24.0	41.0
35	33.864455692356685	37.0	32.0	40.0	23.0	41.0
36	33.86593041749867	37.0	32.0	40.0	23.0	41.0
37	33.71443135001181	37.0	32.0	40.0	22.0	41.0
38	33.56994580625324	37.0	32.0	40.0	21.0	41.0
39	33.44304394616864	37.0	31.0	40.0	20.0	41.0
40	33.17786155245592	36.0	31.0	40.0	18.0	41.0
41	32.95099455866231	36.0	31.0	40.0	18.0	41.0
42	32.73483407855073	36.0	30.0	40.0	15.0	41.0
43	32.55118435574232	36.0	30.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	13.0
12	13.0
13	17.0
14	51.0
15	116.0
16	225.0
17	324.0
18	744.0
19	1414.0
20	2719.0
21	4669.0
22	7973.0
23	12693.0
24	18342.0
25	25367.0
26	34807.0
27	45297.0
28	57924.0
29	72368.0
30	89388.0
31	107863.0
32	128371.0
33	152717.0
34	178828.0
35	205968.0
36	242309.0
37	279390.0
38	310589.0
39	204989.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.476363217069654	23.369840749817435	15.059721106277212	25.094074926835695
2	18.057810323716584	26.322173679885353	31.05218413062139	24.567831865776675
3	19.950153100537545	26.88717231634799	30.54534173540786	22.617332847706603
4	15.969035805209995	18.147263865527762	31.76959696031832	34.11410336894393
5	13.137041910928982	40.95430228067639	32.17719396822317	13.731461840171457
6	33.151391082648665	37.1246840528357	14.818905765841283	14.90501909867435
7	26.971912960559912	33.23512508853842	20.91080635390109	18.882155597000583
8	28.49761060667346	35.52541029662886	19.53560113695223	16.44137795974545
9	28.284706601534115	14.51316225362527	19.159758992483155	38.04237215235746
10	17.697021997792717	29.87144313500118	31.32145988180236	21.110074985403745
11	34.78543961725781	23.85174596841352	19.830088602474866	21.532725811853805
12	20.91432958802869	29.772060478830397	28.62934295801586	20.684266975125052
13	30.296381775819814	20.661709125450926	25.623200633999115	23.41870846473014
14	22.662540059629592	22.586813404029847	25.8581591696515	28.892487366689057
15	26.16747167228249	28.51577585275993	22.088527434554784	23.2282250404028
16	22.871875074353966	27.06891628978738	26.330455567899584	23.72875306795907
17	23.219394076940112	29.122458467017953	25.656968774079246	22.00117868196269
18	23.657647797383838	25.310547922390015	27.686397387865064	23.34540689236108
19	24.439165185688168	27.680677851943635	26.687171584247395	21.192985378120806
20	23.444515010807635	26.04342637721849	27.823803518841522	22.688255093132348
21	23.614225080668337	27.726800189614053	26.908448989975714	21.750525739741896
22	23.71182324163163	27.50657517849528	25.277466126620457	23.50413545325263
23	22.108156881837132	27.224213129126074	26.52276924372178	24.144860745315015
24	22.166770685959957	29.25286388602658	26.11192353941355	22.468441888599912
25	23.209098912281537	27.666173108846888	25.150995748325776	23.9737322305458
26	23.126874863875045	27.692025411211755	26.41977184084865	22.76132788406455
27	23.764671753545656	27.784270086552592	25.36408277861461	23.086975381287143
28	22.514015150821876	28.136364717875885	26.879714041506443	22.4699060897958
29	22.83943386660761	27.621514972372353	27.00856374674444	22.530487414275598
30	22.40918749645389	28.87981287508716	27.23487434408362	21.476125284375325
31	23.537354517884303	27.840230026007873	25.600230977738654	23.022184478369173
32	21.0433165621288	28.91280315828198	26.395475252254414	23.648405027334807
33	21.938812862275405	28.095916159839522	27.075367926306754	22.889903051578315
34	21.716666087087027	28.230942963872668	27.033363654499766	23.019027294540543
35	22.170156651225444	28.97086788695635	27.099161195739907	21.7598142660783
36	22.611979362084146	28.43583961872201	26.243244084169604	22.70893693502424
37	22.01856607116384	28.471392254009626	26.769624414090742	22.7404172607358
38	22.39733661802468	27.838948849961476	27.28296420211101	22.480750329902833
39	22.50060855862204	26.75722446021308	27.107305814892023	23.63486116627286
40	22.30811185765036	27.758051733888756	27.54469016587569	22.38914624258519
41	21.179899079932575	28.02115038627458	27.645674292104477	23.15327624168837
42	22.315753157641392	28.362309264916092	27.598362290962402	21.723575286480116
43	21.926046858098772	26.88822471095753	27.803579239823346	23.38214919112035
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	560.0
1	859.5
2	1159.0
3	1174.0
4	1189.0
5	1189.0
6	1508.0
7	1827.0
8	2901.0
9	3975.0
10	6452.0
11	8929.0
12	8929.0
13	19127.5
14	29326.0
15	37214.5
16	45103.0
17	35497.5
18	25892.0
19	25892.0
20	29432.5
21	32973.0
22	30084.0
23	27195.0
24	29383.0
25	31571.0
26	31571.0
27	35311.5
28	39052.0
29	41873.5
30	44695.0
31	48175.0
32	51655.0
33	51655.0
34	59165.5
35	66676.0
36	75369.0
37	84062.0
38	94228.5
39	104395.0
40	104395.0
41	111404.5
42	118414.0
43	126392.0
44	134370.0
45	151067.0
46	167764.0
47	167764.0
48	206292.0
49	244820.0
50	236322.5
51	227825.0
52	190308.0
53	152791.0
54	152791.0
55	139487.5
56	126184.0
57	119002.0
58	111820.0
59	102898.5
60	93977.0
61	93977.0
62	80036.5
63	66096.0
64	56656.0
65	47216.0
66	39954.5
67	32693.0
68	32693.0
69	26759.5
70	20826.0
71	18366.5
72	15907.0
73	12748.5
74	9590.0
75	9590.0
76	7349.5
77	5109.0
78	4442.0
79	3775.0
80	2929.5
81	2084.0
82	2084.0
83	1761.0
84	1438.0
85	1164.0
86	890.0
87	898.5
88	907.0
89	907.0
90	619.0
91	331.0
92	270.5
93	210.0
94	187.0
95	164.0
96	164.0
97	97.0
98	30.0
99	28.5
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2185492.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.74881932907104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.49511872395493	47.35505627864359
2	6.999018379185939	7.663759854457973
3	2.2904133303192498	3.7619227683163357
4	1.1324968748218334	2.480114671614326
5	0.6766950021761801	1.852412620751451
6	0.464154866830813	1.5247158566897525
7	0.3221584559581549	1.234645657040986
8	0.24093667597856	1.055279883431769
9	0.18193369678461355	0.8964589585617719
>10	1.0289400129326272	10.310530309087724
>50	0.08485071415999411	3.2070249552372516
>100	0.07016239420205281	7.779922125194272
>500	0.007906166880400686	3.0469649093632087
>1k	0.0046259487066174195	4.6558437947663585
>5k	4.205407915106745E-4	1.6601766065267987
>10k+	1.682163166042698E-4	1.515170750316417
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19948	0.9127464204856389	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12956	0.5928184591844765	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	8487	0.3883336109214767	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8272	0.37849600913661546	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	8133	0.3721358851919842	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	6038	0.2762764631488013	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	5123	0.2344094602039266	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4658	0.21313278657620344	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	4601	0.21052467819603093	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	4334	0.1983077494678544	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	4131	0.18901922313145048	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	3513	0.1607418375358958	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	3372	0.1542902010165217	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3159	0.1445441118064033	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	3105	0.14207327228834515	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	2820	0.12903273038748253	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2787	0.12752277290422476	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2560	0.11713609567090613	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2396	0.1096320645419887	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	2349	0.10748151903553066	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	4.575628737144771E-5	0.0	0.0	0.0	0.0
5	4.575628737144771E-5	0.0	0.0	0.0	0.0
6	4.575628737144771E-5	0.0	0.0	0.0	0.0
7	4.575628737144771E-5	0.0	0.0	0.0	0.0
8	4.575628737144771E-5	0.0	0.0	0.0	0.0
9	4.575628737144771E-5	0.0	0.0	0.0	0.0
10	4.575628737144771E-5	0.0	0.0	1.3726886211434314E-4	0.0
11	4.575628737144771E-5	0.0	0.0	1.3726886211434314E-4	0.0
12	4.575628737144771E-5	0.0	0.0	2.2878143685723857E-4	0.0
13	4.575628737144771E-5	0.0	0.0	2.2878143685723857E-4	0.0
14	4.575628737144771E-5	0.0	0.0	3.660502989715817E-4	0.0
15	4.575628737144771E-5	0.0	0.0	5.490754484573726E-4	0.0
16	4.575628737144771E-5	0.0	0.0	0.001281176046400536	0.0
17	4.575628737144771E-5	0.0	0.0	0.002562352092801072	0.0
18	4.575628737144771E-5	0.0	0.0	0.0034317215528585784	0.0
19	4.575628737144771E-5	0.0	0.0	0.005490754484573725	4.575628737144771E-5
20	4.575628737144771E-5	0.0	0.0	0.007092224542574395	4.575628737144771E-5
21	4.575628737144771E-5	0.0	0.0	0.01258297902714812	9.151257474289542E-5
22	4.575628737144771E-5	4.575628737144771E-5	0.0	0.019858228719208306	9.151257474289542E-5
23	4.575628737144771E-5	4.575628737144771E-5	0.0	0.03459175325281447	1.8302514948579084E-4
24	4.575628737144771E-5	4.575628737144771E-5	0.0	0.06474514663059851	1.8302514948579084E-4
25	4.575628737144771E-5	4.575628737144771E-5	0.0	0.0860218202583217	1.8302514948579084E-4
26	4.575628737144771E-5	4.575628737144771E-5	0.0	0.11018113999044608	1.8302514948579084E-4
27	4.575628737144771E-5	4.575628737144771E-5	0.0	0.14545923755383228	1.8302514948579084E-4
28	4.575628737144771E-5	4.575628737144771E-5	0.0	0.20947228358648762	1.8302514948579084E-4
29	4.575628737144771E-5	4.575628737144771E-5	0.0	0.27147205297479926	1.8302514948579084E-4
30	4.575628737144771E-5	4.575628737144771E-5	0.0	0.3597816876016933	1.8302514948579084E-4
31	4.575628737144771E-5	4.575628737144771E-5	0.0	0.5500820867795444	1.8302514948579084E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAGCG	45	0.003826895	20.555555	3
CGTTAAT	55	5.145134E-4	20.181818	1
CGAACGT	155	1.8189894E-12	19.096773	4
ACGTCTG	200	0.0	16.650002	7
TAATACG	90	4.4489076E-5	16.444445	4
GGTTATC	275	0.0	16.145454	2
TCGAACG	185	1.8189894E-11	16.0	3
AGGTTAT	290	0.0	15.948276	1
CTAGAAC	190	2.7284841E-11	15.578948	3
ATACCGT	470	0.0	15.351065	6
GTTATCT	335	0.0	14.910448	3
ACGAACG	250	0.0	14.800001	15
TACACCG	100	1.09408E-4	14.8	5
TATCTAG	465	0.0	14.720429	1
GTAGTAC	115	2.2115366E-5	14.478261	3
ATTCGAA	205	9.276846E-11	14.439025	1
TCTATAC	205	9.276846E-11	14.439025	3
TAGAGTC	590	0.0	14.423729	5
TACCGTC	450	0.0	14.388889	7
ACCGTCG	495	0.0	14.202021	8
>>END_MODULE