FastQCFastQC Report
Thu 9 Feb 2017
SRR2727104.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727104.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2246536
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT261041.161966690050816No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171320.7625962815641503No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG108870.4846127549258058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105600.47005701221792134No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC101490.45176217964012155No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA73940.32912893450182856No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG62470.27807255258762825No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59190.2634722969051019No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT58420.2600447978576796No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG53700.239034673826727No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG52580.23404922066683997No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41650.18539653938329945No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC40820.18170196248802603No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC39870.1774732298970504No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35160.1565076188407397No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG34520.15365878846366138No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA34230.15236791219904777No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32520.14475619353529168No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31190.1388359679079258No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC29570.13162486601594633No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC24490.10901227489788724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT1900.017.5263164
GTATTAG4550.017.4835171
TCGAACG2000.016.653
TAGAGTG2700.015.7592595
ACGAACG2150.015.48837215
CGAACGA2055.456968E-1215.34146316
ATAACGA2201.8189894E-1215.13636412
TAACGAA2109.094947E-1214.9761913
AACGAAC2109.094947E-1214.9761914
TATTAGC4850.014.8762882
AGTACTC9700.014.8762885
ATTCGAA2251.8189894E-1214.81
TACTTAC4150.014.71084331
CGTCGTA4550.014.637362510
CGATAAC2151.2732926E-1114.62790710
TATCTAG4250.014.3647061
CCGATAA2201.8189894E-1114.2954549
CCGTCGT4450.014.1348329
TATAGAG2101.364242E-1014.0952385
AGAGTGT3950.014.0506326
Produced by FastQC (version 0.11.5)