FastQCFastQC Report
Thu 9 Feb 2017
SRR2727103.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727103.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256074
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT155481.2378251599826124No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104380.831001995105384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61300.4880285715650511No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG43340.3450433652794342No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA41290.32872267079805806No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC40140.3195671592597251No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG40030.31869141467779766No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT36970.29432979267145093No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG33100.26351950601636526No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG29380.2339034165184535No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26540.21129328367596178No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24390.19417645775646974No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC22680.18056261016468775No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22540.1794480261513255No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17620.14027835939602284No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA17280.13757151250642877No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC17260.13741228621880558No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17020.13550157076732738No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG16590.132078205583429No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC15960.12706257752329878No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC15750.12539070150325538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATATA803.3828767E-416.18751
ATTAGAG1951.8189894E-1216.1282063
CCGATAA1403.4733603E-815.8571439
ATTCCGA1902.7284841E-1115.5789486
AAGACGG3100.015.5161295
CGACGGT2150.015.4883727
GGTTATC1802.0190782E-1015.4166672
ACGGCTA2400.015.41666624
CTAAGAC1455.34601E-815.3103453
TTTGCGC1101.4520025E-515.13636412
CGTCGTA2450.015.1020410
GACGGTA2350.014.9574468
TATACTG2006.184564E-1114.85
CGAACGT750.00410468914.7999994
CTTATCC1252.9590901E-614.7999993
ACGACGG2400.014.6458336
TAAGGTG1152.2100567E-514.4782615
ACGGTAT2301.8189894E-1214.4782619
CGGCTAC2700.014.38888925
TAGGACT1803.3305696E-914.3888894
Produced by FastQC (version 0.11.5)