##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2727103.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1256074 Sequences flagged as poor quality 0 Sequence length 43 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.938622246778454 31.0 30.0 31.0 27.0 34.0 2 30.380845396051505 31.0 30.0 31.0 27.0 34.0 3 30.687328931257234 31.0 30.0 33.0 27.0 34.0 4 34.628175569273786 35.0 35.0 37.0 32.0 37.0 5 34.29132917328119 35.0 35.0 37.0 30.0 37.0 6 34.25030929706371 35.0 35.0 37.0 30.0 37.0 7 34.08530389133124 35.0 33.0 37.0 30.0 37.0 8 34.10418972130623 35.0 33.0 37.0 30.0 37.0 9 35.370219429746975 37.0 34.0 39.0 30.0 39.0 10 35.18622628921545 37.0 34.0 39.0 29.0 39.0 11 35.23612462323079 37.0 34.0 39.0 30.0 39.0 12 35.02742911643741 37.0 34.0 39.0 29.0 39.0 13 35.208042679014135 37.0 34.0 39.0 30.0 39.0 14 35.81151349363174 38.0 34.0 40.0 27.0 41.0 15 35.88952720938416 38.0 34.0 40.0 29.0 41.0 16 35.68029112934429 38.0 34.0 40.0 27.0 41.0 17 35.72449075452561 38.0 34.0 40.0 27.0 41.0 18 35.618517698798 38.0 33.0 40.0 27.0 41.0 19 35.61606083717997 38.0 34.0 40.0 27.0 41.0 20 36.03205941688149 38.0 34.0 40.0 29.0 41.0 21 36.09056711626863 38.0 34.0 40.0 29.0 41.0 22 36.08089809995271 38.0 34.0 40.0 29.0 41.0 23 35.96777021099076 38.0 34.0 40.0 28.0 41.0 24 35.81342420908322 38.0 34.0 40.0 27.0 41.0 25 35.52437674850367 38.0 34.0 40.0 27.0 41.0 26 35.24329537909391 38.0 34.0 40.0 27.0 41.0 27 35.064450024441236 38.0 33.0 40.0 26.0 41.0 28 34.766723138923346 37.0 33.0 40.0 25.0 41.0 29 34.609960081969696 37.0 33.0 40.0 25.0 41.0 30 34.232478341244224 37.0 33.0 40.0 24.0 41.0 31 34.04809111565083 37.0 32.0 40.0 24.0 41.0 32 33.83729302572937 37.0 32.0 40.0 24.0 41.0 33 33.74338374968354 36.0 32.0 39.0 23.0 41.0 34 33.5311343121504 36.0 31.0 39.0 22.0 40.0 35 33.10130374484306 36.0 31.0 39.0 19.0 40.0 36 33.096894768938775 36.0 31.0 39.0 20.0 40.0 37 32.918313729923554 36.0 31.0 39.0 19.0 40.0 38 32.79461401159486 36.0 30.0 39.0 18.0 40.0 39 32.63028531758479 35.0 30.0 39.0 18.0 40.0 40 32.354313519744856 35.0 30.0 39.0 15.0 40.0 41 32.08872327585795 35.0 30.0 39.0 15.0 40.0 42 31.816926391279495 35.0 30.0 39.0 14.0 40.0 43 31.59574993193076 35.0 29.0 39.0 13.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 6.0 11 12.0 12 15.0 13 18.0 14 46.0 15 96.0 16 144.0 17 334.0 18 613.0 19 1242.0 20 2374.0 21 3868.0 22 6388.0 23 9668.0 24 13615.0 25 18754.0 26 24820.0 27 31896.0 28 39839.0 29 48703.0 30 58478.0 31 69367.0 32 80880.0 33 94059.0 34 107810.0 35 123293.0 36 141304.0 37 156294.0 38 156238.0 39 65897.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.321146684032946 26.232451272775332 16.519249662042203 22.92715238114952 2 19.548370557785606 28.592980986788994 28.348409408999785 23.51023904642561 3 22.15227765243131 26.67135853460863 30.645567060539424 20.53079675242064 4 18.201953069643988 19.14998638615241 30.043930532755237 32.60413001144837 5 13.645135557299968 41.77230003964735 31.151986268324954 13.43057813472773 6 33.497230258726795 37.49938299813546 13.859215301009337 15.14417144212841 7 25.867186168967756 34.263586381057166 20.603085487001565 19.266141962973517 8 28.977910537118035 35.69049275759231 19.44829683601444 15.88329986927522 9 28.225645941242316 14.178941686556684 19.29822605992959 38.29718631227141 10 17.968925397707462 30.885123010268504 31.024764464514032 20.121187127510005 11 34.953673111616034 23.883385851470535 19.06949749775889 22.09344353915454 12 20.214334505769564 31.15509118093361 28.482876008897566 20.147698304399263 13 30.76753439685878 20.594328041182287 26.156420720435257 22.48171684152367 14 22.559021204164722 22.74109646406183 26.6704827900267 28.02939954174675 15 26.40274378738832 29.050597337418015 22.227432460189448 22.319226415004213 16 22.45273765717625 27.30746755366324 26.496130005079316 23.743664784081194 17 22.636245953661966 30.168365876532754 26.153634260401855 21.041753909403425 18 22.871900859344272 25.35073570506196 28.74544015718819 23.03192327840557 19 24.231852581933865 28.2200730211755 27.08956637905092 20.458508017839712 20 23.346793262180412 25.959935481508257 28.549989889130735 22.143281367180595 21 23.210814012550216 28.408039653714667 27.25691320734288 21.124233126392237 22 23.33556780890298 28.265372900004298 25.921880398766316 22.477178892326407 23 21.843458267586147 27.346318767843297 27.594393323960215 23.215829640610348 24 21.865113042702898 29.737340315936798 26.846666677281757 21.55087996407855 25 22.836393397204304 28.235756810506384 25.893378893281767 23.03447089900754 26 22.496445273128813 28.493544170168317 27.161058982193726 21.848951574509144 27 23.23159304308504 28.439407232376436 26.14686714317787 22.18213258136065 28 21.918931527919533 28.460425102342697 28.13026939495603 21.49037397478174 29 22.450030810286655 28.03672395097741 27.723525843222614 21.78971939551332 30 21.868934473605854 29.490300730689434 27.91459738837043 20.72616740733428 31 22.863063800381187 28.866770588357056 26.227196805283764 22.042968805977992 32 20.496563100581653 29.878335193627127 26.941008252698488 22.684093453092732 33 21.618073457455534 28.52284180709098 27.998350415660223 21.86073431979326 34 21.154804573615884 28.95004593678398 27.66883161342405 22.226317876176086 35 21.559159731034956 29.89608892469711 27.81882277636509 20.725928567902844 36 22.126642220123973 29.48815117580652 26.463568229260375 21.921638374809127 37 21.151699661007235 29.72237304490022 27.27450771212524 21.851419581967306 38 21.99679318256727 28.638599318193037 27.689610643958872 21.67499685528082 39 21.834461982335434 27.692954395998964 27.60888291613392 22.86370070553168 40 21.82578414965997 28.73676232451273 27.94572612760076 21.491727398226537 41 20.887782089271813 28.776011604411845 27.914119709507563 22.42208659680879 42 21.722048223273468 29.503277673130725 27.87096938556168 20.903704718034128 43 21.601115857823665 27.817389739776477 28.149058096895562 22.432436305504293 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 361.0 1 522.5 2 684.0 3 722.0 4 760.0 5 760.0 6 1029.0 7 1298.0 8 2028.5 9 2759.0 10 4661.5 11 6564.0 12 6564.0 13 14659.5 14 22755.0 15 28867.5 16 34980.0 17 26680.0 18 18380.0 19 18380.0 20 20769.0 21 23158.0 22 20637.5 23 18117.0 24 18688.0 25 19259.0 26 19259.0 27 21368.0 28 23477.0 29 24488.5 30 25500.0 31 27433.5 32 29367.0 33 29367.0 34 33748.5 35 38130.0 36 43215.0 37 48300.0 38 53357.5 39 58415.0 40 58415.0 41 62529.5 42 66644.0 43 71496.0 44 76348.0 45 86458.5 46 96569.0 47 96569.0 48 121074.5 49 145580.0 50 138004.5 51 130429.0 52 107270.5 53 84112.0 54 84112.0 55 75792.5 56 67473.0 57 63515.0 58 59557.0 59 54372.5 60 49188.0 61 49188.0 62 41589.5 63 33991.0 64 29259.5 65 24528.0 66 20666.5 67 16805.0 68 16805.0 69 13755.0 70 10705.0 71 9525.5 72 8346.0 73 6732.5 74 5119.0 75 5119.0 76 4011.5 77 2904.0 78 2511.0 79 2118.0 80 1651.0 81 1184.0 82 1184.0 83 989.0 84 794.0 85 646.5 86 499.0 87 487.5 88 476.0 89 476.0 90 336.5 91 197.0 92 158.0 93 119.0 94 106.0 95 93.0 96 93.0 97 56.5 98 20.0 99 16.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 1256074.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.92269269871797 #Duplication Level Percentage of deduplicated Percentage of total 1 87.47033147512627 49.790467988136115 2 6.637909760167321 7.556953948796501 3 2.135287173011579 3.646388867185571 4 1.0568564559017244 2.4063646106380014 5 0.6060416497601727 1.7248761295961184 6 0.39894227435297563 1.362532109251324 7 0.28097482527350653 1.1195690544583852 8 0.2069935592339299 0.9426104610309494 9 0.15533397222579778 0.795782517001225 >10 0.886712602978728 9.318819993180066 >50 0.08325113419071914 3.310015586725422 >100 0.06992315337276624 7.868439257650155 >500 0.006921682170692327 2.7040515247797834 >1k 0.004096505774491377 4.870734220866972 >5k 1.412588198100475E-4 0.49290302557019455 >10k+ 2.82517639620095E-4 2.0894907051332914 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 15548 1.2378251599826124 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10438 0.831001995105384 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 6130 0.4880285715650511 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG 4334 0.3450433652794342 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA 4129 0.32872267079805806 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 4014 0.3195671592597251 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 4003 0.31869141467779766 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 3697 0.29432979267145093 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 3310 0.26351950601636526 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG 2938 0.2339034165184535 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2654 0.21129328367596178 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 2439 0.19417645775646974 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC 2268 0.18056261016468775 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2254 0.1794480261513255 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1762 0.14027835939602284 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA 1728 0.13757151250642877 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 1726 0.13741228621880558 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1702 0.13550157076732738 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 1659 0.132078205583429 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 1596 0.12706257752329878 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC 1575 0.12539070150325538 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.961314381159071E-5 0.0 3 0.0 0.0 0.0 7.961314381159071E-5 0.0 4 0.0 0.0 0.0 7.961314381159071E-5 0.0 5 0.0 0.0 0.0 7.961314381159071E-5 0.0 6 7.961314381159071E-5 0.0 0.0 7.961314381159071E-5 0.0 7 7.961314381159071E-5 0.0 0.0 7.961314381159071E-5 0.0 8 7.961314381159071E-5 0.0 0.0 7.961314381159071E-5 0.0 9 7.961314381159071E-5 0.0 0.0 7.961314381159071E-5 0.0 10 7.961314381159071E-5 0.0 0.0 7.961314381159071E-5 0.0 11 7.961314381159071E-5 0.0 0.0 1.5922628762318143E-4 0.0 12 7.961314381159071E-5 0.0 0.0 1.5922628762318143E-4 0.0 13 7.961314381159071E-5 0.0 0.0 1.5922628762318143E-4 0.0 14 7.961314381159071E-5 0.0 0.0 2.3883943143477215E-4 0.0 15 1.5922628762318143E-4 0.0 0.0 8.757445819274979E-4 0.0 16 1.5922628762318143E-4 0.0 0.0 0.0020699417391013587 0.0 17 1.5922628762318143E-4 0.0 0.0 0.003980657190579536 0.0 18 1.5922628762318143E-4 0.0 0.0 0.004936014916318624 7.961314381159071E-5 19 1.5922628762318143E-4 0.0 0.0 0.0069263435116083925 7.961314381159071E-5 20 1.5922628762318143E-4 0.0 0.0 0.008837058963086569 7.961314381159071E-5 21 1.5922628762318143E-4 0.0 0.0 0.014011913310839966 7.961314381159071E-5 22 1.5922628762318143E-4 0.0 0.0 0.022610132842491765 1.5922628762318143E-4 23 1.5922628762318143E-4 0.0 0.0 0.03654243300952014 1.5922628762318143E-4 24 1.5922628762318143E-4 0.0 0.0 0.06743233280841734 1.5922628762318143E-4 25 1.5922628762318143E-4 0.0 0.0 0.08813175019943092 1.5922628762318143E-4 26 1.5922628762318143E-4 0.0 0.0 0.1141652482258211 1.5922628762318143E-4 27 1.5922628762318143E-4 0.0 0.0 0.14529398745615307 1.5922628762318143E-4 28 1.5922628762318143E-4 0.0 0.0 0.20691456076632428 1.5922628762318143E-4 29 1.5922628762318143E-4 0.0 0.0 0.26718171063169843 1.5922628762318143E-4 30 1.5922628762318143E-4 0.0 0.0 0.3577814682892887 1.5922628762318143E-4 31 1.5922628762318143E-4 0.0 0.0 0.5264021068822379 1.5922628762318143E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCATATA 80 3.3828767E-4 16.1875 1 ATTAGAG 195 1.8189894E-12 16.128206 3 CCGATAA 140 3.4733603E-8 15.857143 9 ATTCCGA 190 2.7284841E-11 15.578948 6 AAGACGG 310 0.0 15.516129 5 CGACGGT 215 0.0 15.488372 7 GGTTATC 180 2.0190782E-10 15.416667 2 ACGGCTA 240 0.0 15.416666 24 CTAAGAC 145 5.34601E-8 15.310345 3 TTTGCGC 110 1.4520025E-5 15.136364 12 CGTCGTA 245 0.0 15.10204 10 GACGGTA 235 0.0 14.957446 8 TATACTG 200 6.184564E-11 14.8 5 CGAACGT 75 0.004104689 14.799999 4 CTTATCC 125 2.9590901E-6 14.799999 3 ACGACGG 240 0.0 14.645833 6 TAAGGTG 115 2.2100567E-5 14.478261 5 ACGGTAT 230 1.8189894E-12 14.478261 9 CGGCTAC 270 0.0 14.388889 25 TAGGACT 180 3.3305696E-9 14.388889 4 >>END_MODULE