FastQCFastQC Report
Thu 9 Feb 2017
SRR2727102.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727102.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166022
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT152691.3094950181042897No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101640.8716816663836532No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT57810.495788244132615No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG56510.48463922636108064No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC51210.4391855385232869No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA50910.43661268826831745No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG47400.40651034028517474No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT42500.36448711945400686No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG38550.33061125776357564No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG32980.2828420046963093No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26330.22581049071115294No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25530.21894955669790106No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC25280.21680551481875984No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC23270.19956741811046447No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23200.19896708638430494No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20360.17461077063726071No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA20130.1726382521084508No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC19790.16972235515281875No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG19240.16500546301870805No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC17770.15239849676935768No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16960.14545180108094014No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT14490.12426866731502494No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC14390.12341105056336846No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG14290.12255343381171195No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC13720.11766501832726997No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC13450.11534945309779746No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG12950.11106136933951505No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT12730.10917461248587075No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG12700.10891732746037382No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT12600.10805971070871734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACT1251.8189894E-1120.721
CGAACGT851.2453402E-619.5882364
TAAGATC951.6761624E-719.4736844
ATTCGAA902.15227E-618.51
CGTGTTG609.236924E-418.55
CGAGCCG1552.0008883E-1117.90322515
TCGAACG953.6064775E-617.5263163
CATATAG852.7231703E-517.4117643
TATACTG1603.45608E-1117.343755
TTAGAGT1502.5102054E-1017.2666664
CAAGACG2500.017.024
AGACCGT1201.0412077E-716.9583346
AAGACGG2550.016.6862745
TATCTAG1901.8189894E-1216.5526311
TCATGCA904.446046E-516.4444452
CAATACT904.446046E-516.4444454
TAGAGTG1403.4726327E-815.8571435
ACCGTGA1302.5888585E-715.6538468
CGAGACT1455.3449185E-815.31034520
TAGAGTC2201.8189894E-1215.1363645
Produced by FastQC (version 0.11.5)