##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727102.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1166022
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.007322331825645	31.0	30.0	31.0	27.0	34.0
2	30.434954057470613	31.0	30.0	31.0	27.0	34.0
3	30.736279418398624	31.0	30.0	34.0	27.0	34.0
4	34.657741449132175	35.0	35.0	37.0	32.0	37.0
5	34.31698458519651	35.0	35.0	37.0	30.0	37.0
6	34.2796791141162	35.0	35.0	37.0	30.0	37.0
7	34.10143033321841	35.0	35.0	37.0	30.0	37.0
8	34.12092138913331	35.0	33.0	37.0	30.0	37.0
9	35.388572428307526	37.0	34.0	39.0	30.0	39.0
10	35.21298654742363	37.0	34.0	39.0	29.0	39.0
11	35.281613039891184	37.0	34.0	39.0	30.0	39.0
12	35.08043415990436	37.0	34.0	39.0	29.0	39.0
13	35.24485215544818	37.0	34.0	39.0	30.0	39.0
14	35.83984007162815	38.0	34.0	40.0	28.0	41.0
15	35.92533159751703	38.0	34.0	40.0	29.0	41.0
16	35.695366811260854	38.0	34.0	40.0	27.0	41.0
17	35.77676750524433	38.0	34.0	40.0	27.0	41.0
18	35.67617592120904	38.0	34.0	40.0	27.0	41.0
19	35.665116953196424	38.0	34.0	40.0	27.0	41.0
20	36.11249787739854	38.0	34.0	40.0	29.0	41.0
21	36.19347405108994	38.0	34.0	40.0	29.0	41.0
22	36.20319942505373	38.0	34.0	40.0	29.0	41.0
23	36.06195080367266	38.0	34.0	40.0	29.0	41.0
24	35.94562109462772	38.0	34.0	40.0	28.0	41.0
25	35.65215664884539	38.0	34.0	40.0	27.0	41.0
26	35.38806043110679	38.0	34.0	40.0	27.0	41.0
27	35.2089334506553	38.0	34.0	40.0	27.0	41.0
28	34.92596280344625	38.0	33.0	40.0	25.0	41.0
29	34.75815207603287	37.0	33.0	40.0	25.0	41.0
30	34.37160190802575	37.0	33.0	40.0	24.0	41.0
31	34.1782324861795	37.0	33.0	40.0	24.0	41.0
32	33.93851573984024	37.0	32.0	40.0	24.0	41.0
33	33.87473907010331	36.0	32.0	40.0	24.0	41.0
34	33.65779376375403	36.0	32.0	39.0	23.0	40.0
35	33.23835485093763	36.0	31.0	39.0	20.0	40.0
36	33.2484335630031	36.0	31.0	39.0	21.0	40.0
37	33.058720161369166	36.0	31.0	39.0	19.0	40.0
38	32.93172169993362	36.0	31.0	39.0	19.0	40.0
39	32.750322892707	35.0	30.0	39.0	18.0	40.0
40	32.4788254424016	35.0	30.0	39.0	16.0	40.0
41	32.20503815536928	35.0	30.0	39.0	15.0	40.0
42	31.944450447761707	35.0	30.0	39.0	15.0	40.0
43	31.693824816341372	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	4.0
12	9.0
13	14.0
14	31.0
15	68.0
16	134.0
17	266.0
18	561.0
19	1071.0
20	1979.0
21	3502.0
22	5382.0
23	8152.0
24	11850.0
25	16498.0
26	22069.0
27	29036.0
28	36631.0
29	44276.0
30	53821.0
31	63405.0
32	74999.0
33	86857.0
34	99905.0
35	114122.0
36	131527.0
37	146300.0
38	147444.0
39	66104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.87901600484382	25.84127915253743	16.25466757917089	23.02503726344786
2	18.565001346458303	29.107769836246653	28.36190054733101	23.965328269964033
3	21.948470955093473	27.35814590119226	30.799676163914576	19.893706979799695
4	17.817845632415168	19.637708379430233	29.80106721828576	32.74337876986883
5	13.491340643658525	42.02939567177978	30.87780504999048	13.601458634571218
6	32.99414590805319	36.920658443837254	14.339866657747452	15.745328990362104
7	25.179713590309618	34.55680939124648	20.800808218026763	19.462668800417145
8	28.99988164888827	35.403277125131424	19.94216232626829	15.654678899712012
9	28.24920970616335	14.445267756526034	19.765579037102217	37.5399435002084
10	18.383615403482953	31.103787064051964	30.65902701664291	19.85357051582217
11	33.93469419959486	24.335990230029967	19.174423810185402	22.554891760189776
12	20.021234590771016	32.03232872107044	28.498347372519557	19.448089315638985
13	30.558428571673605	21.0024339163412	26.491695696993713	21.947441814991482
14	22.62633123560276	23.256079216344116	27.033452199015112	27.084137349038013
15	26.509448363753002	28.8430235450103	22.563982497757333	22.083545593479368
16	21.752848574040627	27.53627290051131	27.50436955734969	23.206508968098372
17	22.43551150835919	30.754479761102278	26.445555915754593	20.364452814783938
18	22.63499316479449	25.28897396447065	29.35313398889558	22.722898881839278
19	23.88205368337819	29.07140688597642	27.51843447207686	19.528104958568534
20	22.866978496117568	26.367255506328352	29.30244883887268	21.4633171586814
21	22.66552432115346	29.020893259303854	27.847244734661952	20.46633768488073
22	23.126579086843986	28.913176595295802	25.816150981713896	22.144093336146316
23	21.19050926997947	27.9557332537465	27.786525468644673	23.06723200762936
24	21.04720151077767	30.89255605811897	27.10978008991254	20.950462341190818
25	22.204812602163596	28.965834263847505	25.846510614722533	22.98284251926636
26	21.885521885521886	29.02235120778167	27.54021793756893	21.551908969127513
27	22.900511311107337	29.008029008029006	26.35181840479854	21.739641276065118
28	21.394707818548877	29.183325872067595	28.554092461377227	20.867873848006298
29	21.95678983758454	28.38445586789958	28.24809480438619	21.41065949012969
30	21.180132107284425	30.311434947196535	28.588911701494485	19.919521244024555
31	22.42273301875951	29.43649433715659	26.358593577136624	21.78217906694728
32	19.564124862138108	30.7359552392665	27.33361806209488	22.36630183650051
33	20.901235139645735	29.379720108196928	28.52767786542621	21.191366886731124
34	20.260338141132845	29.664620393097213	28.104186713458233	21.970854752311705
35	20.524827147343704	30.795473841831456	28.45598110498773	20.22371790583711
36	21.614686515348765	30.013584649346235	26.724195598367785	21.64753323693721
37	20.355190553866052	30.41743637770128	27.753078415330073	21.4742946531026
38	21.34599518705479	29.369600230527382	28.179914272629503	21.104490309788325
39	21.28570472941334	27.938152110337537	28.06525091293303	22.71089224731609
40	21.016584592743534	29.291814391152137	28.44483208721619	21.246768928888134
41	20.003481924011727	29.42405889425757	28.395519123995943	22.176940057734758
42	20.882967902835453	30.227474267209363	28.35812703362372	20.53143079633146
43	20.8621278157702	28.15512914850663	28.77227016299864	22.21047287272453
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	532.0
1	707.5
2	883.0
3	872.0
4	861.0
5	861.0
6	1173.0
7	1485.0
8	2268.0
9	3051.0
10	4976.5
11	6902.0
12	6902.0
13	15068.5
14	23235.0
15	29407.0
16	35579.0
17	27497.5
18	19416.0
19	19416.0
20	21594.0
21	23772.0
22	21338.5
23	18905.0
24	19412.0
25	19919.0
26	19919.0
27	21719.5
28	23520.0
29	24512.5
30	25505.0
31	27087.5
32	28670.0
33	28670.0
34	32329.5
35	35989.0
36	39818.0
37	43647.0
38	47384.0
39	51121.0
40	51121.0
41	55276.0
42	59431.0
43	64160.0
44	68889.0
45	79564.0
46	90239.0
47	90239.0
48	118486.5
49	146734.0
50	138186.0
51	129638.0
52	102094.0
53	74550.0
54	74550.0
55	65477.5
56	56405.0
57	52783.5
58	49162.0
59	44653.5
60	40145.0
61	40145.0
62	34034.0
63	27923.0
64	24125.0
65	20327.0
66	17275.5
67	14224.0
68	14224.0
69	11482.5
70	8741.0
71	7603.0
72	6465.0
73	5207.0
74	3949.0
75	3949.0
76	3062.0
77	2175.0
78	1814.5
79	1454.0
80	1167.0
81	880.0
82	880.0
83	751.5
84	623.0
85	497.5
86	372.0
87	358.5
88	345.0
89	345.0
90	261.0
91	177.0
92	132.5
93	88.0
94	75.5
95	63.0
96	63.0
97	40.0
98	17.0
99	15.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1166022.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.324837695553306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.55871981661804	48.441719626848204
2	6.579401384469592	7.28008627459358
3	2.1278493676361996	3.5316876271517548
4	1.046501922063771	2.315901959450508
5	0.6061532896575919	1.6767666184465995
6	0.39006272831427835	1.2948094281043294
7	0.26648126791158566	1.0320123027279615
8	0.20239997153374242	0.8958196459751128
9	0.15069190074328842	0.7503304455591174
>10	0.8927380758339036	9.304046497726084
>50	0.09225692812724785	3.544053176981378
>100	0.07372400331602402	7.863431388281252
>500	0.007215539923431315	2.8010722630661675
>1k	0.004862646470138495	5.18281245762734
>5k	6.274382542114186E-4	1.87831607837792
>10k+	3.137191271057093E-4	2.2071342090826853
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15269	1.3094950181042897	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10164	0.8716816663836532	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5781	0.495788244132615	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	5651	0.48463922636108064	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	5121	0.4391855385232869	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	5091	0.43661268826831745	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	4740	0.40651034028517474	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	4250	0.36448711945400686	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	3855	0.33061125776357564	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	3298	0.2828420046963093	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2633	0.22581049071115294	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2553	0.21894955669790106	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	2528	0.21680551481875984	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	2327	0.19956741811046447	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2320	0.19896708638430494	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2036	0.17461077063726071	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	2013	0.1726382521084508	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	1979	0.16972235515281875	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	1924	0.16500546301870805	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	1777	0.15239849676935768	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1696	0.14545180108094014	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	1449	0.12426866731502494	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	1439	0.12341105056336846	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	1429	0.12255343381171195	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC	1372	0.11766501832726997	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC	1345	0.11534945309779746	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	1295	0.11106136933951505	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	1273	0.10917461248587075	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	1270	0.10891732746037382	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	1260	0.10805971070871734	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	8.576167516564867E-5
10	0.0	0.0	0.0	8.576167516564867E-5	8.576167516564867E-5
11	0.0	0.0	0.0	2.57285025496946E-4	8.576167516564867E-5
12	0.0	0.0	0.0	4.288083758282434E-4	8.576167516564867E-5
13	0.0	0.0	0.0	6.003317261595408E-4	8.576167516564867E-5
14	0.0	0.0	0.0	7.718550764908381E-4	1.7152335033129734E-4
15	0.0	0.0	0.0	0.0016294718281473248	1.7152335033129734E-4
16	0.0	0.0	0.0	0.0028301352804664063	1.7152335033129734E-4
17	0.0	0.0	0.0	0.006089078936761056	1.7152335033129734E-4
18	0.0	0.0	0.0	0.007804312440074029	1.7152335033129734E-4
19	0.0	0.0	0.0	0.010119877669546544	1.7152335033129734E-4
20	0.0	0.0	0.0	0.012692727924516005	1.7152335033129734E-4
21	0.0	0.0	0.0	0.02015399366392744	1.7152335033129734E-4
22	0.0	0.0	0.0	0.03327552996427169	1.7152335033129734E-4
23	0.0	0.0	0.0	0.05454442540535256	2.57285025496946E-4
24	0.0	0.0	0.0	0.096910692937183	2.57285025496946E-4
25	0.0	0.0	0.0	0.12658423254449744	2.57285025496946E-4
26	0.0	0.0	0.0	0.16371903789122333	2.57285025496946E-4
27	0.0	0.0	0.0	0.2123459077101461	2.57285025496946E-4
28	0.0	0.0	0.0	0.2887595602827391	2.57285025496946E-4
29	0.0	0.0	0.0	0.3662881146324855	2.57285025496946E-4
30	0.0	0.0	0.0	0.48283823118260205	2.57285025496946E-4
31	0.0	0.0	0.0	0.6980142741732146	2.57285025496946E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAACT	125	1.8189894E-11	20.72	1
CGAACGT	85	1.2453402E-6	19.588236	4
TAAGATC	95	1.6761624E-7	19.473684	4
ATTCGAA	90	2.15227E-6	18.5	1
CGTGTTG	60	9.236924E-4	18.5	5
CGAGCCG	155	2.0008883E-11	17.903225	15
TCGAACG	95	3.6064775E-6	17.526316	3
CATATAG	85	2.7231703E-5	17.411764	3
TATACTG	160	3.45608E-11	17.34375	5
TTAGAGT	150	2.5102054E-10	17.266666	4
CAAGACG	250	0.0	17.02	4
AGACCGT	120	1.0412077E-7	16.958334	6
AAGACGG	255	0.0	16.686274	5
TATCTAG	190	1.8189894E-12	16.552631	1
TCATGCA	90	4.446046E-5	16.444445	2
CAATACT	90	4.446046E-5	16.444445	4
TAGAGTG	140	3.4726327E-8	15.857143	5
ACCGTGA	130	2.5888585E-7	15.653846	8
CGAGACT	145	5.3449185E-8	15.310345	20
TAGAGTC	220	1.8189894E-12	15.136364	5
>>END_MODULE