FastQCFastQC Report
Thu 9 Feb 2017
SRR2727101.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727101.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3764641
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT283980.7543348754901198No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193100.5129307150402921No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG143750.3818425183171516No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC139020.3692782392796551No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT122980.32667125497490995No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA102170.2713937398014844No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG82000.21781625392700127No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT76020.20193160516500777No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74070.19675182839479247No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG70800.18806574119550842No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG68050.1807609278016151No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC58600.155658932684418No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC50640.13451481827882128No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG50560.13430231461645348No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA47120.1251646571346378No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44960.11942705825070704No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40520.10763310498929379No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39930.10606589047933122No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT39900.1059862016059433No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC39820.10577369794357551No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38770.10298458737499805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC2900.018.50000223
CGAACGT2750.018.1636354
GGTTATC5700.017.8508782
GTAATAC2600.017.0769233
AAGACGG10800.016.9583325
CAAGACG10900.016.4633034
TCGAACG3100.016.1129043
AGGTTAT6700.015.1865671
ATTCGAA3550.015.1126761
GTTATCT7350.015.102043
CGAACGA5900.015.05084716
TATCTAG10400.014.7644231
TAACGCC9150.014.7595634
TTAGAGT6800.014.6911764
TAATACC3950.014.5189874
ATAACGC9950.014.5025133
TACCGTC9450.014.4867737
ATACCGT9500.014.4105266
TAGAGTG6550.014.404585
TCGTTTA8900.014.34269630
Produced by FastQC (version 0.11.5)