##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727101.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3764641
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.41554400539122	31.0	30.0	31.0	27.0	34.0
2	30.80560058714762	31.0	31.0	34.0	27.0	34.0
3	31.14819580406206	31.0	31.0	34.0	28.0	34.0
4	35.04918583206207	35.0	35.0	37.0	32.0	37.0
5	34.760725126247095	35.0	35.0	37.0	32.0	37.0
6	34.65207465997422	35.0	35.0	37.0	31.0	37.0
7	34.501533346738775	35.0	35.0	37.0	30.0	37.0
8	34.50514644025818	35.0	35.0	37.0	30.0	37.0
9	35.86560019932843	37.0	35.0	39.0	30.0	39.0
10	35.69714323357792	37.0	35.0	39.0	30.0	39.0
11	35.69651873844013	37.0	35.0	39.0	30.0	39.0
12	35.53140976789022	37.0	35.0	39.0	30.0	39.0
13	35.64794093248201	37.0	35.0	39.0	30.0	39.0
14	36.39228893272958	38.0	35.0	40.0	30.0	41.0
15	36.387201063793334	38.0	35.0	40.0	30.0	41.0
16	36.23003282384695	38.0	34.0	40.0	30.0	41.0
17	36.215912221112184	38.0	34.0	40.0	30.0	41.0
18	36.08797810999774	38.0	34.0	40.0	29.0	41.0
19	36.018284346369285	38.0	34.0	40.0	28.0	41.0
20	36.485843138827846	38.0	35.0	40.0	30.0	41.0
21	36.576214040063846	38.0	35.0	40.0	30.0	41.0
22	36.55584768906252	38.0	35.0	40.0	30.0	41.0
23	36.46183713134931	38.0	35.0	40.0	30.0	41.0
24	36.3137515635621	38.0	35.0	40.0	30.0	41.0
25	36.009566383620644	38.0	34.0	40.0	28.0	41.0
26	35.78370022533357	38.0	34.0	40.0	27.0	41.0
27	35.62366717038889	38.0	34.0	40.0	27.0	41.0
28	35.34909278202092	38.0	34.0	40.0	27.0	41.0
29	35.18036168654594	38.0	34.0	40.0	26.0	41.0
30	34.84011436947109	38.0	33.0	40.0	25.0	41.0
31	34.68929812962245	38.0	33.0	40.0	25.0	41.0
32	34.46450883364443	37.0	33.0	40.0	24.0	41.0
33	34.403216136678104	37.0	33.0	40.0	24.0	41.0
34	34.2023704252278	37.0	33.0	40.0	24.0	41.0
35	33.787561682508375	37.0	32.0	40.0	22.0	41.0
36	33.78163389284662	37.0	32.0	40.0	22.0	41.0
37	33.626843037622976	37.0	32.0	40.0	21.0	41.0
38	33.49233140689909	37.0	31.0	40.0	21.0	41.0
39	33.35091101648205	37.0	31.0	40.0	19.0	41.0
40	33.1096638431128	37.0	31.0	40.0	18.0	41.0
41	32.87974789628015	36.0	31.0	40.0	16.0	41.0
42	32.68050154051874	36.0	30.0	40.0	15.0	41.0
43	32.516558949445646	36.0	30.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	15.0
11	22.0
12	29.0
13	31.0
14	96.0
15	174.0
16	352.0
17	729.0
18	1382.0
19	2795.0
20	5012.0
21	8997.0
22	14764.0
23	22423.0
24	32971.0
25	45252.0
26	60677.0
27	79383.0
28	101165.0
29	125563.0
30	153056.0
31	184431.0
32	220908.0
33	260138.0
34	306329.0
35	353615.0
36	414362.0
37	478050.0
38	536714.0
39	355205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.58109763985464	23.263227489686265	14.883225253085222	25.272449617373876
2	18.172489754003106	25.994908943508822	31.415611740933596	24.416989561554477
3	19.77107511712272	27.01771563344287	30.474486146222173	22.73672310321223
4	15.86127335913305	18.214937360561073	32.246341683044946	33.67744759726094
5	13.226201382814457	40.793053042773536	32.35086692197211	13.629878652439901
6	33.054997807227835	37.420487106207474	14.950721728844796	14.5737933577199
7	27.006904509619904	33.401113147309395	21.04630959499193	18.54567274807877
8	28.205372039458744	35.639122030493745	19.631885218271808	16.523620711775706
9	28.397900357563977	14.676193560023387	19.063172291859967	37.862733790552674
10	17.652015158948757	29.5667236265025	31.473386174139844	21.3078750404089
11	35.087196893408965	23.777380100785177	19.899241388488303	21.236181617317563
12	21.065195857984865	29.44501215388134	28.66063457312397	20.829157415009824
13	30.116550289921403	20.78277849069805	25.430897660626872	23.669773558753676
14	22.65060599403768	22.709177315977804	25.510374030352427	29.12984265963209
15	26.149292854219038	28.572365864367942	21.98722268604098	23.291118595372044
16	23.24208337528067	27.184849763895148	25.88092729160629	23.692139569217886
17	23.472995167401088	28.909582613587858	25.280179438092503	22.337242780918555
18	23.963931753386312	25.307406469833378	27.292721935504606	23.4359398412757
19	24.648485738746402	27.559414031776203	26.264124520771038	21.527975708706354
20	23.69984282697872	25.828359198128055	27.299282986080215	23.172514988813013
21	23.97028030029955	27.611105547647174	26.459548201276036	21.959065950777244
22	23.91364807427853	27.178421528108526	25.004721565748234	23.90320883186471
23	22.388243659886825	26.89815576040318	26.269012105005494	24.444588474704492
24	22.496248646285263	28.84155487867236	25.863236361714172	22.798960113328203
25	23.52394292045377	27.2254379634074	24.917249745726085	24.333369370412743
26	23.409270631648543	27.407341098394244	26.164885310445275	23.018502959511945
27	24.174124438425867	27.357641804357975	25.064249154169016	23.403984603047142
28	22.90399004845349	27.751198587063147	26.49989733416812	22.84491403031524
29	23.18691211193843	27.27797949392784	26.73713111024398	22.797977283889754
30	22.856362665125307	28.469806284317684	26.78268658286408	21.891144467692936
31	23.96103639098655	27.36258251450802	25.3105408988533	23.365840195652122
32	21.520511517565684	28.461784271063294	26.01209517720282	24.0056090341682
33	22.405934589778944	27.481053306278074	26.752139181398704	23.360872922544274
34	22.362318213077952	27.711460402200373	26.787866359634293	23.138355025087385
35	22.79396627726256	28.303070598232345	26.849014288480628	22.05394883602447
36	23.086743198089803	27.852217515561247	26.049681762484123	23.011357523864824
37	22.553784012871347	27.98269476425508	26.543301207206742	22.920220015666832
38	22.97276154618727	27.117672043629128	27.02247040288835	22.887096007295252
39	23.04952849421764	26.259582254987922	26.83785784620632	23.853031404588112
40	22.846401555951815	27.142827164661913	27.303214303834018	22.70755697555225
41	21.590823666851634	27.55024981133659	27.46623117582792	23.392695345983853
42	22.858939272031517	27.76219565159068	27.40115192922778	21.97771314715002
43	22.31362831143793	26.29244063378155	27.51091007084075	23.88302098393977
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1197.0
1	1563.0
2	1929.0
3	1957.5
4	1986.0
5	1986.0
6	2573.5
7	3161.0
8	4729.0
9	6297.0
10	10177.0
11	14057.0
12	14057.0
13	29389.0
14	44721.0
15	55145.0
16	65569.0
17	52778.5
18	39988.0
19	39988.0
20	45835.0
21	51682.0
22	47921.5
23	44161.0
24	48460.0
25	52759.0
26	52759.0
27	58694.0
28	64629.0
29	69983.5
30	75338.0
31	82011.0
32	88684.0
33	88684.0
34	102198.0
35	115712.0
36	132546.0
37	149380.0
38	166141.0
39	182902.0
40	182902.0
41	194896.5
42	206891.0
43	218777.0
44	230663.0
45	258924.0
46	287185.0
47	287185.0
48	350292.0
49	413399.0
50	399690.0
51	385981.0
52	325051.0
53	264121.0
54	264121.0
55	243067.5
56	222014.0
57	211079.0
58	200144.0
59	184600.5
60	169057.0
61	169057.0
62	143463.5
63	117870.0
64	102625.5
65	87381.0
66	73998.5
67	60616.0
68	60616.0
69	49627.0
70	38638.0
71	34466.0
72	30294.0
73	24216.5
74	18139.0
75	18139.0
76	13881.0
77	9623.0
78	8226.5
79	6830.0
80	5390.5
81	3951.0
82	3951.0
83	3350.0
84	2749.0
85	2229.5
86	1710.0
87	1701.0
88	1692.0
89	1692.0
90	1207.0
91	722.0
92	566.5
93	411.0
94	352.5
95	294.0
96	294.0
97	177.0
98	60.0
99	57.0
100	54.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3764641.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.4071591150291
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.30136340372583	43.85100761224254
2	7.719626002991603	7.936880844886109
3	2.431653745488298	3.750132330209202
4	1.2430809631959256	2.556130434715063
5	0.7234537992402615	1.859535228495822
6	0.5001286927044086	1.5426117170308213
7	0.34359206202526743	1.2364164262235708
8	0.25071282397273603	1.0310747227315777
9	0.19235898090578413	0.889976586476564
>10	1.1160319177635678	10.607209792264632
>50	0.08779423387635577	3.1844886141840516
>100	0.07213421363219674	7.592365062669878
>500	0.010799957991244473	3.83970836425133
>1k	0.006542282237912186	6.076637699841656
>5k	4.1538299923251965E-4	1.416655184252608
>10k+	3.115372494243898E-4	2.6291693795244737
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28398	0.7543348754901198	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19310	0.5129307150402921	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	14375	0.3818425183171516	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	13902	0.3692782392796551	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12298	0.32667125497490995	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	10217	0.2713937398014844	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	8200	0.21781625392700127	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	7602	0.20193160516500777	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7407	0.19675182839479247	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	7080	0.18806574119550842	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG	6805	0.1807609278016151	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	5860	0.155658932684418	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	5064	0.13451481827882128	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	5056	0.13430231461645348	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	4712	0.1251646571346378	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4496	0.11942705825070704	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	4052	0.10763310498929379	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3993	0.10606589047933122	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	3990	0.1059862016059433	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	3982	0.10577369794357551	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3877	0.10298458737499805	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.312591559195153E-5
2	0.0	0.0	0.0	0.0	5.312591559195153E-5
3	0.0	0.0	0.0	0.0	5.312591559195153E-5
4	0.0	0.0	0.0	0.0	5.312591559195153E-5
5	0.0	0.0	0.0	0.0	5.312591559195153E-5
6	0.0	0.0	0.0	0.0	7.96888733879273E-5
7	0.0	0.0	0.0	0.0	7.96888733879273E-5
8	0.0	0.0	0.0	0.0	7.96888733879273E-5
9	0.0	0.0	0.0	5.312591559195153E-5	1.0625183118390306E-4
10	0.0	0.0	0.0	1.3281478897987883E-4	1.0625183118390306E-4
11	2.6562957795975765E-5	0.0	0.0	2.1250366236780612E-4	1.0625183118390306E-4
12	2.6562957795975765E-5	0.0	0.0	2.1250366236780612E-4	1.0625183118390306E-4
13	2.6562957795975765E-5	5.312591559195153E-5	0.0	2.390666201637819E-4	1.0625183118390306E-4
14	5.312591559195153E-5	5.312591559195153E-5	0.0	3.718814091436607E-4	1.3281478897987883E-4
15	7.96888733879273E-5	5.312591559195153E-5	0.0	5.046961981235395E-4	1.3281478897987883E-4
16	7.96888733879273E-5	5.312591559195153E-5	0.0	0.0015140885943706185	1.3281478897987883E-4
17	7.96888733879273E-5	5.312591559195153E-5	0.0	0.0030016142309452613	1.3281478897987883E-4
18	7.96888733879273E-5	5.312591559195153E-5	0.0	0.003851628880416486	1.8594070457183035E-4
19	7.96888733879273E-5	5.312591559195153E-5	0.0	0.005684472968338814	1.8594070457183035E-4
20	7.96888733879273E-5	5.312591559195153E-5	0.0	0.0073845022672812625	1.8594070457183035E-4
21	7.96888733879273E-5	5.312591559195153E-5	0.0	0.012590841995292512	1.8594070457183035E-4
22	7.96888733879273E-5	5.312591559195153E-5	0.0	0.020851921869840976	1.8594070457183035E-4
23	1.0625183118390306E-4	5.312591559195153E-5	0.0	0.03761314823910168	3.187554935517092E-4
24	1.0625183118390306E-4	5.312591559195153E-5	0.0	0.0656636316716521	3.187554935517092E-4
25	1.0625183118390306E-4	5.312591559195153E-5	0.0	0.08611710917455342	3.453184513476849E-4
26	1.0625183118390306E-4	5.312591559195153E-5	0.0	0.1086159344277449	3.453184513476849E-4
27	1.0625183118390306E-4	5.312591559195153E-5	0.0	0.1408368022342635	3.453184513476849E-4
28	1.0625183118390306E-4	5.312591559195153E-5	0.0	0.20323319009701057	3.453184513476849E-4
29	1.0625183118390306E-4	5.312591559195153E-5	0.0	0.26220295640407676	3.453184513476849E-4
30	1.0625183118390306E-4	5.312591559195153E-5	0.0	0.347443487971363	3.453184513476849E-4
31	1.0625183118390306E-4	5.312591559195153E-5	0.0	0.5329591852184578	3.453184513476849E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	290	0.0	18.500002	23
CGAACGT	275	0.0	18.163635	4
GGTTATC	570	0.0	17.850878	2
GTAATAC	260	0.0	17.076923	3
AAGACGG	1080	0.0	16.958332	5
CAAGACG	1090	0.0	16.463303	4
TCGAACG	310	0.0	16.112904	3
AGGTTAT	670	0.0	15.186567	1
ATTCGAA	355	0.0	15.112676	1
GTTATCT	735	0.0	15.10204	3
CGAACGA	590	0.0	15.050847	16
TATCTAG	1040	0.0	14.764423	1
TAACGCC	915	0.0	14.759563	4
TTAGAGT	680	0.0	14.691176	4
TAATACC	395	0.0	14.518987	4
ATAACGC	995	0.0	14.502513	3
TACCGTC	945	0.0	14.486773	7
ATACCGT	950	0.0	14.410526	6
TAGAGTG	655	0.0	14.40458	5
TCGTTTA	890	0.0	14.342696	30
>>END_MODULE