FastQCFastQC Report
Thu 9 Feb 2017
SRR2727100.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727100.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3007145
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT223710.7439282109775218No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139930.465325084091389No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG93170.30982875784174024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92760.30846533838574464No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC88050.29280264170833137No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA64300.21382407565980355No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG57260.1904131659763663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54380.18083597565132378No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT50200.16693574802678288No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG47010.15632767957647536No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG45180.1502421732241046No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC37270.12393815396331072No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35760.118916779869278No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG34880.11599041615884835No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA32870.10930633541116241No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC31930.10618044690229438No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT31150.10358662452259536No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCCG450.003827224520.5555575
TATCTAG7600.016.5526311
CGTTAAT904.4498018E-516.4444451
AAGACGG8700.016.3735645
ATACCGT7500.016.0333336
ACTACGC1059.3530725E-615.8571423
CGAACGT3200.015.6093754
CGTCGTA7500.015.53999910
CAAGACG9300.015.118284
ACTCTAA6000.015.10833410
TACCGTC7350.015.1020417
TAGAGTC9550.014.916235
CGTGCGA1508.1146936E-814.832
CCGTCGT7400.014.7500019
AGGTTAT4900.014.7244891
CACTCTA6300.014.6825399
ACCGTCG7600.014.6052648
TATTTCG908.279497E-414.3888895
CGAGCCG6600.014.29545415
GTATTAG6650.013.9097741
Produced by FastQC (version 0.11.5)