##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2727100.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3007145 Sequences flagged as poor quality 0 Sequence length 43 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.45075312297877 31.0 30.0 31.0 28.0 34.0 2 30.848105096362165 31.0 31.0 34.0 28.0 34.0 3 31.174363723731314 31.0 31.0 34.0 28.0 34.0 4 35.07460099197079 35.0 35.0 37.0 32.0 37.0 5 34.78786257396966 35.0 35.0 37.0 32.0 37.0 6 34.66573909804815 35.0 35.0 37.0 31.0 37.0 7 34.522219247824765 35.0 35.0 37.0 30.0 37.0 8 34.51729896629527 35.0 35.0 37.0 30.0 37.0 9 35.86748494003449 37.0 35.0 39.0 30.0 39.0 10 35.70296510477546 37.0 35.0 39.0 30.0 39.0 11 35.693058033450335 37.0 35.0 39.0 30.0 39.0 12 35.51873687500935 37.0 35.0 39.0 30.0 39.0 13 35.630715845095594 37.0 35.0 39.0 30.0 39.0 14 36.37702737979046 38.0 35.0 40.0 30.0 41.0 15 36.36788116303005 38.0 35.0 40.0 30.0 41.0 16 36.21451709179305 38.0 34.0 40.0 30.0 41.0 17 36.189055399722996 38.0 34.0 40.0 30.0 41.0 18 36.05941416193765 38.0 34.0 40.0 29.0 41.0 19 35.985159012950824 38.0 34.0 40.0 27.0 41.0 20 36.45193896536416 38.0 35.0 40.0 30.0 41.0 21 36.54307923296017 38.0 35.0 40.0 30.0 41.0 22 36.51627473899662 38.0 35.0 40.0 30.0 41.0 23 36.42061057913735 38.0 35.0 40.0 30.0 41.0 24 36.26241767523681 38.0 35.0 40.0 29.0 41.0 25 35.946063791403475 38.0 34.0 40.0 27.0 41.0 26 35.72306722821813 38.0 34.0 40.0 27.0 41.0 27 35.55462673066979 38.0 34.0 40.0 27.0 41.0 28 35.27641400730593 38.0 34.0 40.0 26.0 41.0 29 35.09599071544605 38.0 33.0 40.0 25.0 41.0 30 34.756004116861675 38.0 33.0 40.0 25.0 41.0 31 34.5990974828284 38.0 33.0 40.0 24.0 41.0 32 34.396062045561486 37.0 33.0 40.0 24.0 41.0 33 34.32118105379022 37.0 33.0 40.0 24.0 41.0 34 34.118049512078734 37.0 33.0 40.0 24.0 41.0 35 33.691009578853034 37.0 32.0 40.0 21.0 41.0 36 33.666215297233755 37.0 32.0 40.0 21.0 41.0 37 33.51550324310933 37.0 31.0 40.0 21.0 41.0 38 33.37557350909251 37.0 31.0 40.0 20.0 41.0 39 33.231577792224854 37.0 31.0 40.0 19.0 41.0 40 32.982474074246504 36.0 31.0 40.0 18.0 41.0 41 32.74858345706642 36.0 30.0 40.0 16.0 41.0 42 32.5397056011599 36.0 30.0 39.0 15.0 41.0 43 32.36803147171154 36.0 30.0 39.0 15.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 10.0 11 21.0 12 33.0 13 35.0 14 87.0 15 162.0 16 323.0 17 568.0 18 1122.0 19 2244.0 20 4293.0 21 7551.0 22 12406.0 23 18953.0 24 27234.0 25 38083.0 26 50496.0 27 64958.0 28 82632.0 29 101808.0 30 124125.0 31 148782.0 32 175434.0 33 208570.0 34 242423.0 35 279282.0 36 327659.0 37 377350.0 38 424494.0 39 286007.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.42727570502919 23.307988141576146 15.031400215154243 25.233335938240426 2 18.438552181554265 25.85897919787706 31.3561201737861 24.346348446782578 3 19.560513377306382 26.77882177281109 30.477146928398863 23.183517921483666 4 15.772501824820553 18.087155757371196 32.12189635019263 34.01844606761563 5 13.158294661547748 40.67253158726965 32.39650898110999 13.772664770072609 6 33.34325414970013 37.394339148926974 14.688683119703242 14.573723581669656 7 27.371443678306168 33.156997750357895 20.84342457713213 18.628133994203804 8 28.250915735689496 35.501447386142004 19.47325453212266 16.774382346045837 9 28.258530932163232 14.534749737708024 18.936599332589548 38.27011999753919 10 17.600148978516167 29.409489731955063 31.445507283486496 21.54485400604228 11 35.32463516059252 23.62839171373512 19.78275074863367 21.264222377038685 12 21.06483059513259 29.2189435494464 28.4591863711261 21.257039484294904 13 30.217299132565937 20.702493561168485 25.321060341287165 23.75914696497841 14 22.6701406151017 22.488340269591255 25.28551167303206 29.556007442274986 15 26.08277951345878 28.698948670582897 21.696127057391646 23.52214475856668 16 23.369109238164437 27.09094506583487 25.48420511814362 24.055740577857073 17 23.51971720685235 28.858169459736725 25.03613892911715 22.585974404293772 18 24.009218045687852 25.324585279392913 26.923676776477357 23.742519898441877 19 24.660799529121473 27.407025600694347 26.017933953966306 21.914240916217874 20 23.894757319650367 25.8273212631915 26.957828771143394 23.320092646014743 21 24.28599219525497 27.445001820663784 26.040114460726038 22.22889152335521 22 24.172562347342748 26.97299265582471 24.849649750843408 24.004795245989136 23 22.67363229907437 26.761729148411533 25.969482682078848 24.59515587043525 24 22.80066308741348 28.617742077618473 25.523145707972176 23.05844912699587 25 23.704876219803168 27.119842907475363 24.847887281790534 24.32739359093093 26 23.748239609330447 27.26945325217108 25.8415207780137 23.14078636048478 27 24.34295652520913 27.321928274160374 24.852210319090034 23.482904881540463 28 23.11943720705187 27.64203255912169 26.18463692306158 23.053893310764863 29 23.360729196630025 27.222631432804206 26.4654680768636 22.951171293702167 30 23.075508497262355 28.358160314850135 26.36916410748401 22.197167080403503 31 23.98673825173046 27.358308295742304 25.028556986776497 23.626396465750737 32 21.804003465080665 28.320317111412983 25.67172517454263 24.20395424896372 33 22.524986324237773 27.492289197893683 26.382864810310114 23.59985966755843 34 22.577461346227068 27.624806918189844 26.384594025229912 23.413137710353176 35 23.06905719544618 28.053053643904768 26.492104637455128 22.385784523193927 36 23.22305708570754 27.741462417010155 25.763805869021944 23.27167462826036 37 22.736515864715535 27.777875692725157 26.240104817027447 23.245503625531857 38 23.066164085868824 26.993776488995376 26.757605635910476 23.18245378922533 39 23.186111743863368 26.201130973065816 26.511126001572922 24.101631281497898 40 22.903917170605343 27.051671934675582 26.994707604721423 23.049703289997655 41 21.809124601573917 27.373704959355138 27.203011494291097 23.614158944779852 42 22.925864898433566 27.57439365245108 27.15635594558959 22.34338550352577 43 22.407133676626835 26.266741377618967 27.288607632821165 24.037517312933034 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 997.0 1 1237.0 2 1477.0 3 1424.0 4 1371.0 5 1371.0 6 1828.5 7 2286.0 8 3569.0 9 4852.0 10 7720.5 11 10589.0 12 10589.0 13 21930.5 14 33272.0 15 42045.5 16 50819.0 17 40611.0 18 30403.0 19 30403.0 20 34844.5 21 39286.0 22 36294.5 23 33303.0 24 36511.5 25 39720.0 26 39720.0 27 44523.5 28 49327.0 29 53270.5 30 57214.0 31 62518.5 32 67823.0 33 67823.0 34 78631.5 35 89440.0 36 102946.5 37 116453.0 38 130101.0 39 143749.0 40 143749.0 41 154291.5 42 164834.0 43 176308.5 44 187783.0 45 210474.5 46 233166.0 47 233166.0 48 278025.0 49 322884.0 50 312145.5 51 301407.0 52 258701.0 53 215995.0 54 215995.0 55 200205.0 56 184415.0 57 175429.0 58 166443.0 59 153255.5 60 140068.0 61 140068.0 62 119367.5 63 98667.0 64 85712.5 65 72758.0 66 61775.5 67 50793.0 68 50793.0 69 41315.5 70 31838.0 71 28480.0 72 25122.0 73 20199.5 74 15277.0 75 15277.0 76 11620.5 77 7964.0 78 6846.0 79 5728.0 80 4453.5 81 3179.0 82 3179.0 83 2740.5 84 2302.0 85 1863.0 86 1424.0 87 1432.5 88 1441.0 89 1441.0 90 991.5 91 542.0 92 450.5 93 359.0 94 314.5 95 270.0 96 270.0 97 161.0 98 52.0 99 52.5 100 53.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 3007145.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.827208035791884 #Duplication Level Percentage of deduplicated Percentage of total 1 85.26309495845871 45.04211255145976 2 7.588638796866539 8.017732008611002 3 2.5113940501273238 3.980098078377782 4 1.2287192273492626 2.5963922496302785 5 0.7423591715379081 1.9608381196055593 6 0.5084078359429755 1.611465990983179 7 0.35177366109245 1.3008254263230106 8 0.2636102880676188 1.11406364224985 9 0.19915009589024443 0.9468489191347659 >10 1.16987809497151 11.310465560395766 >50 0.08774999139759289 3.2037565505240506 >100 0.0698623365913164 7.611006598155863 >500 0.00910310619661345 3.302890993310915 >1k 0.005689441372827172 5.116954688485655 >5k 4.425121067754467E-4 1.6701635375694708 >10k+ 1.2643203050727048E-4 1.2143850851830806 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 22371 0.7439282109775218 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13993 0.465325084091389 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG 9317 0.30982875784174024 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 9276 0.30846533838574464 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 8805 0.29280264170833137 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA 6430 0.21382407565980355 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 5726 0.1904131659763663 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 5438 0.18083597565132378 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 5020 0.16693574802678288 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 4701 0.15632767957647536 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG 4518 0.1502421732241046 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC 3727 0.12393815396331072 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3576 0.118916779869278 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG 3488 0.11599041615884835 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA 3287 0.10930633541116241 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 3193 0.10618044690229438 No Hit GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT 3115 0.10358662452259536 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.325413307306432E-5 2 0.0 0.0 0.0 3.325413307306432E-5 3.325413307306432E-5 3 0.0 0.0 0.0 3.325413307306432E-5 3.325413307306432E-5 4 0.0 0.0 0.0 3.325413307306432E-5 3.325413307306432E-5 5 0.0 0.0 0.0 3.325413307306432E-5 3.325413307306432E-5 6 0.0 0.0 0.0 3.325413307306432E-5 1.3301653229225727E-4 7 0.0 0.0 0.0 3.325413307306432E-5 1.3301653229225727E-4 8 0.0 0.0 0.0 3.325413307306432E-5 1.3301653229225727E-4 9 0.0 0.0 0.0 6.650826614612864E-5 1.3301653229225727E-4 10 0.0 0.0 0.0 6.650826614612864E-5 1.3301653229225727E-4 11 0.0 0.0 0.0 1.662706653653216E-4 1.662706653653216E-4 12 0.0 0.0 0.0 2.3277893151145022E-4 1.662706653653216E-4 13 0.0 0.0 0.0 2.6603306458451454E-4 1.662706653653216E-4 14 6.650826614612864E-5 0.0 0.0 4.3230372994983613E-4 2.3277893151145022E-4 15 6.650826614612864E-5 0.0 0.0 7.980991937535436E-4 2.6603306458451454E-4 16 6.650826614612864E-5 0.0 0.0 0.0019287397182377304 3.325413307306432E-4 17 6.650826614612864E-5 0.0 0.0 0.0032256509080872387 3.325413307306432E-4 18 6.650826614612864E-5 0.0 0.0 0.003990495968767718 3.325413307306432E-4 19 6.650826614612864E-5 0.0 0.0 0.006018998086224641 3.325413307306432E-4 20 6.650826614612864E-5 3.325413307306432E-5 0.0 0.008247025002119952 3.325413307306432E-4 21 6.650826614612864E-5 3.325413307306432E-5 0.0 0.01240379163625299 3.325413307306432E-4 22 6.650826614612864E-5 3.325413307306432E-5 0.0 0.021282645166761163 3.325413307306432E-4 23 9.976239921919295E-5 3.325413307306432E-5 0.0 0.03707835837646672 3.990495968767718E-4 24 9.976239921919295E-5 3.325413307306432E-5 0.0 0.06447976402867171 3.990495968767718E-4 25 9.976239921919295E-5 3.325413307306432E-5 0.0 0.0829358078842224 3.990495968767718E-4 26 9.976239921919295E-5 3.325413307306432E-5 0.0 0.10445123198249502 3.990495968767718E-4 27 9.976239921919295E-5 3.325413307306432E-5 0.0 0.13547733813966403 3.990495968767718E-4 28 9.976239921919295E-5 3.325413307306432E-5 0.0 0.1958003355342027 3.990495968767718E-4 29 9.976239921919295E-5 3.325413307306432E-5 0.0 0.25662214492483737 3.990495968767718E-4 30 9.976239921919295E-5 3.325413307306432E-5 0.0 0.34637505008903796 3.990495968767718E-4 31 9.976239921919295E-5 3.325413307306432E-5 0.0 0.5416100653609985 4.3230372994983613E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATCCG 45 0.0038272245 20.555557 5 TATCTAG 760 0.0 16.552631 1 CGTTAAT 90 4.4498018E-5 16.444445 1 AAGACGG 870 0.0 16.373564 5 ATACCGT 750 0.0 16.033333 6 ACTACGC 105 9.3530725E-6 15.857142 3 CGAACGT 320 0.0 15.609375 4 CGTCGTA 750 0.0 15.539999 10 CAAGACG 930 0.0 15.11828 4 ACTCTAA 600 0.0 15.108334 10 TACCGTC 735 0.0 15.102041 7 TAGAGTC 955 0.0 14.91623 5 CGTGCGA 150 8.1146936E-8 14.8 32 CCGTCGT 740 0.0 14.750001 9 AGGTTAT 490 0.0 14.724489 1 CACTCTA 630 0.0 14.682539 9 ACCGTCG 760 0.0 14.605264 8 TATTTCG 90 8.279497E-4 14.388889 5 CGAGCCG 660 0.0 14.295454 15 GTATTAG 665 0.0 13.909774 1 >>END_MODULE