FastQCFastQC Report
Thu 9 Feb 2017
SRR2727099.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727099.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199668
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT229200.7163243186480597No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160420.5013645165685939No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC159350.4980204196185355No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG154720.4835501683299642No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA114130.35669325692540604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT95020.29696831046221045No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG90430.2826230721437349No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT82810.2588081013405141No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG77930.24355651898884512No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC71190.2224918335277285No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG70700.2209604246440568No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG64350.2011146156413728No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC63730.19917691460489026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55800.17439309328342814No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA53670.1677361526258349No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC45740.14295233130437285No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC45420.14195222754360765No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT44790.1399832732646012No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34030.10635478430887206No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33210.10379201842191127No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT32290.10091672010971138No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT32240.10076045389709183No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT32080.10026040201670924No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGT303.600902E-430.83333231
TATACCG1002.8789145E-718.4999985
GGTTATC4700.018.1063842
TATTCCG852.7255755E-517.4117645
AGGTTAT5350.016.5981311
ATACCGT6950.015.7050366
TATCTAG9550.015.69111
TACCGTC6700.015.4626877
CGAACGT2900.015.3103454
CTAGAGT11500.015.1217394
GTGCGAT1752.242814E-914.833
TAGAGTC10650.014.7652585
AGTACTC16300.014.414115
ACCGTCG6950.014.3741018
CAAGACG8400.014.3154754
CCGTCGT6900.014.2101459
CGTCGTA7100.014.07042210
TTATCTA6050.014.0661164
TCGAACG3300.014.0151513
TCGACTA800.006301861313.87527
Produced by FastQC (version 0.11.5)